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Single-pot enzymatic synthesis of Dicer-substrate siRNAs

We describe an inexpensive and efficient method for generating functional pools of Dicer-substrate small interfering RNAs (siRNAs) in a single reaction tube. The method exploits a highly active form of the enzyme Dicer from Giardia lamblia, which is capable of accurately processing double-stranded R...

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Detalles Bibliográficos
Autores principales:	Guiley, Keelan Z., Pratt, Ashley J., MacRae, Ian J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2012
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3299999/ https://www.ncbi.nlm.nih.gov/pubmed/22189103 http://dx.doi.org/10.1093/nar/gkr1174

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