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Interplay between HIV-1 infection and host microRNAs
Using microRNA array analyses of in vitro HIV-1-infected CD4(+) cells, we find that several host microRNAs are significantly up- or downregulated around the time HIV-1 infection peaks in vitro. While microRNA-223 levels were significantly enriched in HIV-1-infected CD4(+)CD8(−) PBMCs, microRNA-29a/b...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3300021/ https://www.ncbi.nlm.nih.gov/pubmed/22080513 http://dx.doi.org/10.1093/nar/gkr961 |
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author | Sun, Guihua Li, Haitang Wu, Xiwei Covarrubias, Maricela Scherer, Lisa Meinking, Keith Luk, Brian Chomchan, Pritsana Alluin, Jessica Gombart, Adrian F. Rossi, John J. |
author_facet | Sun, Guihua Li, Haitang Wu, Xiwei Covarrubias, Maricela Scherer, Lisa Meinking, Keith Luk, Brian Chomchan, Pritsana Alluin, Jessica Gombart, Adrian F. Rossi, John J. |
author_sort | Sun, Guihua |
collection | PubMed |
description | Using microRNA array analyses of in vitro HIV-1-infected CD4(+) cells, we find that several host microRNAs are significantly up- or downregulated around the time HIV-1 infection peaks in vitro. While microRNA-223 levels were significantly enriched in HIV-1-infected CD4(+)CD8(−) PBMCs, microRNA-29a/b, microRNA-155 and microRNA-21 levels were significantly reduced. Based on the potential for microRNA binding sites in a conserved sequence of the Nef-3′-LTR, several host microRNAs potentially could affect HIV-1 gene expression. Among those microRNAs, the microRNA-29 family has seed complementarity in the HIV-1 3′-UTR, but the potential suppressive effect of microRNA-29 on HIV-1 is severely blocked by the secondary structure of the target region. Our data support a possible regulatory circuit at the peak of HIV-1 replication which involves downregulation of microRNA-29, expression of Nef, the apoptosis of host CD4 cells and upregulation of microRNA-223. |
format | Online Article Text |
id | pubmed-3300021 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-33000212012-03-13 Interplay between HIV-1 infection and host microRNAs Sun, Guihua Li, Haitang Wu, Xiwei Covarrubias, Maricela Scherer, Lisa Meinking, Keith Luk, Brian Chomchan, Pritsana Alluin, Jessica Gombart, Adrian F. Rossi, John J. Nucleic Acids Res RNA Using microRNA array analyses of in vitro HIV-1-infected CD4(+) cells, we find that several host microRNAs are significantly up- or downregulated around the time HIV-1 infection peaks in vitro. While microRNA-223 levels were significantly enriched in HIV-1-infected CD4(+)CD8(−) PBMCs, microRNA-29a/b, microRNA-155 and microRNA-21 levels were significantly reduced. Based on the potential for microRNA binding sites in a conserved sequence of the Nef-3′-LTR, several host microRNAs potentially could affect HIV-1 gene expression. Among those microRNAs, the microRNA-29 family has seed complementarity in the HIV-1 3′-UTR, but the potential suppressive effect of microRNA-29 on HIV-1 is severely blocked by the secondary structure of the target region. Our data support a possible regulatory circuit at the peak of HIV-1 replication which involves downregulation of microRNA-29, expression of Nef, the apoptosis of host CD4 cells and upregulation of microRNA-223. Oxford University Press 2012-03 2011-11-10 /pmc/articles/PMC3300021/ /pubmed/22080513 http://dx.doi.org/10.1093/nar/gkr961 Text en © The Author(s) 2011. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | RNA Sun, Guihua Li, Haitang Wu, Xiwei Covarrubias, Maricela Scherer, Lisa Meinking, Keith Luk, Brian Chomchan, Pritsana Alluin, Jessica Gombart, Adrian F. Rossi, John J. Interplay between HIV-1 infection and host microRNAs |
title | Interplay between HIV-1 infection and host microRNAs |
title_full | Interplay between HIV-1 infection and host microRNAs |
title_fullStr | Interplay between HIV-1 infection and host microRNAs |
title_full_unstemmed | Interplay between HIV-1 infection and host microRNAs |
title_short | Interplay between HIV-1 infection and host microRNAs |
title_sort | interplay between hiv-1 infection and host micrornas |
topic | RNA |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3300021/ https://www.ncbi.nlm.nih.gov/pubmed/22080513 http://dx.doi.org/10.1093/nar/gkr961 |
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