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A supramolecular assembly formed by influenza A virus genomic RNA segments

The influenza A virus genome consists of eight viral RNAs (vRNAs) that form viral ribonucleoproteins (vRNPs). Even though evidence supporting segment-specific packaging of vRNAs is accumulating, the mechanism ensuring selective packaging of one copy of each vRNA into the viral particles remains larg...

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Autores principales: Fournier, Emilie, Moules, Vincent, Essere, Boris, Paillart, Jean-Christophe, Sirbat, Jean-Daniel, Isel, Catherine, Cavalier, Annie, Rolland, Jean-Paul, Thomas, Daniel, Lina, Bruno, Marquet, Roland
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3300030/
https://www.ncbi.nlm.nih.gov/pubmed/22075989
http://dx.doi.org/10.1093/nar/gkr985
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author Fournier, Emilie
Moules, Vincent
Essere, Boris
Paillart, Jean-Christophe
Sirbat, Jean-Daniel
Isel, Catherine
Cavalier, Annie
Rolland, Jean-Paul
Thomas, Daniel
Lina, Bruno
Marquet, Roland
author_facet Fournier, Emilie
Moules, Vincent
Essere, Boris
Paillart, Jean-Christophe
Sirbat, Jean-Daniel
Isel, Catherine
Cavalier, Annie
Rolland, Jean-Paul
Thomas, Daniel
Lina, Bruno
Marquet, Roland
author_sort Fournier, Emilie
collection PubMed
description The influenza A virus genome consists of eight viral RNAs (vRNAs) that form viral ribonucleoproteins (vRNPs). Even though evidence supporting segment-specific packaging of vRNAs is accumulating, the mechanism ensuring selective packaging of one copy of each vRNA into the viral particles remains largely unknown. We used electron tomography to show that the eight vRNPs emerge from a common ‘transition zone’ located underneath the matrix layer at the budding tip of the virions, where they appear to be interconnected and often form a star-like structure. This zone appears as a platform in 3D surface rendering and is thick enough to contain all known packaging signals. In vitro, all vRNA segments are involved in a single network of intermolecular interactions. The regions involved in the strongest interactions were identified and correspond to known packaging signals. A limited set of nucleotides in the 5′ region of vRNA 7 was shown to interact with vRNA 6 and to be crucial for packaging of the former vRNA. Collectively, our findings support a model in which the eight genomic RNA segments are selected and packaged as an organized supramolecular complex held together by direct base pairing of the packaging signals.
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spelling pubmed-33000302012-03-13 A supramolecular assembly formed by influenza A virus genomic RNA segments Fournier, Emilie Moules, Vincent Essere, Boris Paillart, Jean-Christophe Sirbat, Jean-Daniel Isel, Catherine Cavalier, Annie Rolland, Jean-Paul Thomas, Daniel Lina, Bruno Marquet, Roland Nucleic Acids Res RNA The influenza A virus genome consists of eight viral RNAs (vRNAs) that form viral ribonucleoproteins (vRNPs). Even though evidence supporting segment-specific packaging of vRNAs is accumulating, the mechanism ensuring selective packaging of one copy of each vRNA into the viral particles remains largely unknown. We used electron tomography to show that the eight vRNPs emerge from a common ‘transition zone’ located underneath the matrix layer at the budding tip of the virions, where they appear to be interconnected and often form a star-like structure. This zone appears as a platform in 3D surface rendering and is thick enough to contain all known packaging signals. In vitro, all vRNA segments are involved in a single network of intermolecular interactions. The regions involved in the strongest interactions were identified and correspond to known packaging signals. A limited set of nucleotides in the 5′ region of vRNA 7 was shown to interact with vRNA 6 and to be crucial for packaging of the former vRNA. Collectively, our findings support a model in which the eight genomic RNA segments are selected and packaged as an organized supramolecular complex held together by direct base pairing of the packaging signals. Oxford University Press 2012-03 2011-11-09 /pmc/articles/PMC3300030/ /pubmed/22075989 http://dx.doi.org/10.1093/nar/gkr985 Text en © The Author(s) 2011. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle RNA
Fournier, Emilie
Moules, Vincent
Essere, Boris
Paillart, Jean-Christophe
Sirbat, Jean-Daniel
Isel, Catherine
Cavalier, Annie
Rolland, Jean-Paul
Thomas, Daniel
Lina, Bruno
Marquet, Roland
A supramolecular assembly formed by influenza A virus genomic RNA segments
title A supramolecular assembly formed by influenza A virus genomic RNA segments
title_full A supramolecular assembly formed by influenza A virus genomic RNA segments
title_fullStr A supramolecular assembly formed by influenza A virus genomic RNA segments
title_full_unstemmed A supramolecular assembly formed by influenza A virus genomic RNA segments
title_short A supramolecular assembly formed by influenza A virus genomic RNA segments
title_sort supramolecular assembly formed by influenza a virus genomic rna segments
topic RNA
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3300030/
https://www.ncbi.nlm.nih.gov/pubmed/22075989
http://dx.doi.org/10.1093/nar/gkr985
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