Biocatalytic hydroxylation of n-butane with in situ cofactor regeneration at low temperature and under normal pressure

The hydroxylation of n-alkanes, which proceeds in the presence of a P450-monooxygenase advantageously at temperatures significantly below room temperature, is described. In addition, an enzymatic hydroxylation of the “liquid gas” n-butane with in situ cofactor regeneration, which does not require hi...

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Autores principales: Staudt, Svenja, Müller, Christina A, Marienhagen, Jan, Böing, Christian, Buchholz, Stefan, Schwaneberg, Ulrich, Gröger, Harald
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Beilstein-Institut 2012
Materias:
Letter
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3302080/
https://www.ncbi.nlm.nih.gov/pubmed/22423286
http://dx.doi.org/10.3762/bjoc.8.20
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author Staudt, Svenja
Müller, Christina A
Marienhagen, Jan
Böing, Christian
Buchholz, Stefan
Schwaneberg, Ulrich
Gröger, Harald
author_facet Staudt, Svenja
Müller, Christina A
Marienhagen, Jan
Böing, Christian
Buchholz, Stefan
Schwaneberg, Ulrich
Gröger, Harald
author_sort Staudt, Svenja
collection PubMed
description The hydroxylation of n-alkanes, which proceeds in the presence of a P450-monooxygenase advantageously at temperatures significantly below room temperature, is described. In addition, an enzymatic hydroxylation of the “liquid gas” n-butane with in situ cofactor regeneration, which does not require high-pressure conditions, was developed. The resulting 2-butanol was obtained as the only regioisomer, at a product concentration of 0.16 g/L.
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spelling pubmed-33020802012-03-15 Biocatalytic hydroxylation of n-butane with in situ cofactor regeneration at low temperature and under normal pressure Staudt, Svenja Müller, Christina A Marienhagen, Jan Böing, Christian Buchholz, Stefan Schwaneberg, Ulrich Gröger, Harald Beilstein J Org Chem Letter The hydroxylation of n-alkanes, which proceeds in the presence of a P450-monooxygenase advantageously at temperatures significantly below room temperature, is described. In addition, an enzymatic hydroxylation of the “liquid gas” n-butane with in situ cofactor regeneration, which does not require high-pressure conditions, was developed. The resulting 2-butanol was obtained as the only regioisomer, at a product concentration of 0.16 g/L. Beilstein-Institut 2012-02-02 /pmc/articles/PMC3302080/ /pubmed/22423286 http://dx.doi.org/10.3762/bjoc.8.20 Text en Copyright © 2012, Staudt et al. https://creativecommons.org/licenses/by/2.0https://www.beilstein-journals.org/bjoc/termsThis is an Open Access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The license is subject to the Beilstein Journal of Organic Chemistry terms and conditions: (https://www.beilstein-journals.org/bjoc/terms)
spellingShingle Letter
Staudt, Svenja
Müller, Christina A
Marienhagen, Jan
Böing, Christian
Buchholz, Stefan
Schwaneberg, Ulrich
Gröger, Harald
Biocatalytic hydroxylation of n-butane with in situ cofactor regeneration at low temperature and under normal pressure
title Biocatalytic hydroxylation of n-butane with in situ cofactor regeneration at low temperature and under normal pressure
title_full Biocatalytic hydroxylation of n-butane with in situ cofactor regeneration at low temperature and under normal pressure
title_fullStr Biocatalytic hydroxylation of n-butane with in situ cofactor regeneration at low temperature and under normal pressure
title_full_unstemmed Biocatalytic hydroxylation of n-butane with in situ cofactor regeneration at low temperature and under normal pressure
title_short Biocatalytic hydroxylation of n-butane with in situ cofactor regeneration at low temperature and under normal pressure
title_sort biocatalytic hydroxylation of n-butane with in situ cofactor regeneration at low temperature and under normal pressure
topic Letter
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3302080/
https://www.ncbi.nlm.nih.gov/pubmed/22423286
http://dx.doi.org/10.3762/bjoc.8.20
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