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The volatiles of pathogenic and nonpathogenic mycobacteria and related bacteria

Volatiles released by pathogenic and nonpathogenic mycobacteria, as well as by mycobacteria-related Nocardia spp., were analyzed. Bacteria were cultivated on solid and in liquid media, and headspace samples were collected at various times during the bacterial lifecycle to elucidate the conditions gi...

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Autores principales: Nawrath, Thorben, Mgode, Georgies F, Weetjens, Bart, Kaufmann, Stefan H E, Schulz, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Beilstein-Institut 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3302092/
https://www.ncbi.nlm.nih.gov/pubmed/22423297
http://dx.doi.org/10.3762/bjoc.8.31
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author Nawrath, Thorben
Mgode, Georgies F
Weetjens, Bart
Kaufmann, Stefan H E
Schulz, Stefan
author_facet Nawrath, Thorben
Mgode, Georgies F
Weetjens, Bart
Kaufmann, Stefan H E
Schulz, Stefan
author_sort Nawrath, Thorben
collection PubMed
description Volatiles released by pathogenic and nonpathogenic mycobacteria, as well as by mycobacteria-related Nocardia spp., were analyzed. Bacteria were cultivated on solid and in liquid media, and headspace samples were collected at various times during the bacterial lifecycle to elucidate the conditions giving optimal volatile emission. Emitted volatiles were collected by using closed-loop stripping analysis (CLSA) and were analyzed by gas-chromatography–mass-spectrometry. A wide range of compounds was produced, although the absolute amount was small. Nevertheless, characteristic bouquets of compounds could be identified. Predominantly aromatic compounds and fatty-acid derivatives were released by pathogenic/nonpathogenic mycobacteria, while the two Nocardia spp. (N. asteroides and N. africana) emitted the sesquiterpene aciphyllene. Pathogenic Mycobacterium tuberculosis strains grown on agar plates produced a distinct bouquet with different volatiles, while liquid cultures produce less compounds but sometimes an earlier onset of volatile production because of their steeper growth curves under this conditions. This behavior differentiates M. tuberculosis from other mycobacteria, which generally produced fewer compounds in seemingly lower amounts. Knowledge of the production of volatiles by M. tuberculosis can facilitate the rational design of alternative and faster diagnostic measures for tuberculosis.
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spelling pubmed-33020922012-03-15 The volatiles of pathogenic and nonpathogenic mycobacteria and related bacteria Nawrath, Thorben Mgode, Georgies F Weetjens, Bart Kaufmann, Stefan H E Schulz, Stefan Beilstein J Org Chem Full Research Paper Volatiles released by pathogenic and nonpathogenic mycobacteria, as well as by mycobacteria-related Nocardia spp., were analyzed. Bacteria were cultivated on solid and in liquid media, and headspace samples were collected at various times during the bacterial lifecycle to elucidate the conditions giving optimal volatile emission. Emitted volatiles were collected by using closed-loop stripping analysis (CLSA) and were analyzed by gas-chromatography–mass-spectrometry. A wide range of compounds was produced, although the absolute amount was small. Nevertheless, characteristic bouquets of compounds could be identified. Predominantly aromatic compounds and fatty-acid derivatives were released by pathogenic/nonpathogenic mycobacteria, while the two Nocardia spp. (N. asteroides and N. africana) emitted the sesquiterpene aciphyllene. Pathogenic Mycobacterium tuberculosis strains grown on agar plates produced a distinct bouquet with different volatiles, while liquid cultures produce less compounds but sometimes an earlier onset of volatile production because of their steeper growth curves under this conditions. This behavior differentiates M. tuberculosis from other mycobacteria, which generally produced fewer compounds in seemingly lower amounts. Knowledge of the production of volatiles by M. tuberculosis can facilitate the rational design of alternative and faster diagnostic measures for tuberculosis. Beilstein-Institut 2012-02-22 /pmc/articles/PMC3302092/ /pubmed/22423297 http://dx.doi.org/10.3762/bjoc.8.31 Text en Copyright © 2012, Nawrath et al. https://creativecommons.org/licenses/by/2.0https://www.beilstein-journals.org/bjoc/termsThis is an Open Access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The license is subject to the Beilstein Journal of Organic Chemistry terms and conditions: (https://www.beilstein-journals.org/bjoc/terms)
spellingShingle Full Research Paper
Nawrath, Thorben
Mgode, Georgies F
Weetjens, Bart
Kaufmann, Stefan H E
Schulz, Stefan
The volatiles of pathogenic and nonpathogenic mycobacteria and related bacteria
title The volatiles of pathogenic and nonpathogenic mycobacteria and related bacteria
title_full The volatiles of pathogenic and nonpathogenic mycobacteria and related bacteria
title_fullStr The volatiles of pathogenic and nonpathogenic mycobacteria and related bacteria
title_full_unstemmed The volatiles of pathogenic and nonpathogenic mycobacteria and related bacteria
title_short The volatiles of pathogenic and nonpathogenic mycobacteria and related bacteria
title_sort volatiles of pathogenic and nonpathogenic mycobacteria and related bacteria
topic Full Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3302092/
https://www.ncbi.nlm.nih.gov/pubmed/22423297
http://dx.doi.org/10.3762/bjoc.8.31
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