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The coupling between sister kinetochore directional instability and oscillations in centromere stretch in metaphase PtK1 cells
Kinetochores bound to kinetochore microtubules (kMTs) exhibit directional instability in mammalian and other mitotic vertebrate cells, oscillating between poleward (P) and away-from-the-pole (AP) movements. These oscillations are coupled to changes in length of kMTs in a way that maintains a net str...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3302731/ https://www.ncbi.nlm.nih.gov/pubmed/22298429 http://dx.doi.org/10.1091/mbc.E11-09-0767 |
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author | Wan, Xiaohu Cimini, Daniela Cameron, Lisa A. Salmon, E. D. |
author_facet | Wan, Xiaohu Cimini, Daniela Cameron, Lisa A. Salmon, E. D. |
author_sort | Wan, Xiaohu |
collection | PubMed |
description | Kinetochores bound to kinetochore microtubules (kMTs) exhibit directional instability in mammalian and other mitotic vertebrate cells, oscillating between poleward (P) and away-from-the-pole (AP) movements. These oscillations are coupled to changes in length of kMTs in a way that maintains a net stretch of the centromere. To understand how sister kinetochore directional instability and kMT plus-end dynamic instability are coupled to oscillations in centromere stretch, we tracked at high resolution the positions of fluorescent kinetochores and their poles for oscillating chromosomes within spindles of metaphase PtK1 cells. We found that the kinetics of P and AP movement are nonlinear and different. By subtracting contributions from the poleward flux of kMTs, we found that maximum centromere stretch occurred when the leading kinetochore switched from depolymerization to polymerization, whereas minimum centromere stretch occurred on average 7 s after the initially trailing kinetochore switched from polymerization to depolymerization. These differences produce oscillations in centromere stretch at about twice the frequency of kinetochore directional instability and at about twice the frequency of centromere oscillations back and forth across the spindle equator. |
format | Online Article Text |
id | pubmed-3302731 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-33027312012-05-30 The coupling between sister kinetochore directional instability and oscillations in centromere stretch in metaphase PtK1 cells Wan, Xiaohu Cimini, Daniela Cameron, Lisa A. Salmon, E. D. Mol Biol Cell Articles Kinetochores bound to kinetochore microtubules (kMTs) exhibit directional instability in mammalian and other mitotic vertebrate cells, oscillating between poleward (P) and away-from-the-pole (AP) movements. These oscillations are coupled to changes in length of kMTs in a way that maintains a net stretch of the centromere. To understand how sister kinetochore directional instability and kMT plus-end dynamic instability are coupled to oscillations in centromere stretch, we tracked at high resolution the positions of fluorescent kinetochores and their poles for oscillating chromosomes within spindles of metaphase PtK1 cells. We found that the kinetics of P and AP movement are nonlinear and different. By subtracting contributions from the poleward flux of kMTs, we found that maximum centromere stretch occurred when the leading kinetochore switched from depolymerization to polymerization, whereas minimum centromere stretch occurred on average 7 s after the initially trailing kinetochore switched from polymerization to depolymerization. These differences produce oscillations in centromere stretch at about twice the frequency of kinetochore directional instability and at about twice the frequency of centromere oscillations back and forth across the spindle equator. The American Society for Cell Biology 2012-03-15 /pmc/articles/PMC3302731/ /pubmed/22298429 http://dx.doi.org/10.1091/mbc.E11-09-0767 Text en © 2012 Wan et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology. |
spellingShingle | Articles Wan, Xiaohu Cimini, Daniela Cameron, Lisa A. Salmon, E. D. The coupling between sister kinetochore directional instability and oscillations in centromere stretch in metaphase PtK1 cells |
title | The coupling between sister kinetochore directional instability and oscillations in centromere stretch in metaphase PtK1 cells |
title_full | The coupling between sister kinetochore directional instability and oscillations in centromere stretch in metaphase PtK1 cells |
title_fullStr | The coupling between sister kinetochore directional instability and oscillations in centromere stretch in metaphase PtK1 cells |
title_full_unstemmed | The coupling between sister kinetochore directional instability and oscillations in centromere stretch in metaphase PtK1 cells |
title_short | The coupling between sister kinetochore directional instability and oscillations in centromere stretch in metaphase PtK1 cells |
title_sort | coupling between sister kinetochore directional instability and oscillations in centromere stretch in metaphase ptk1 cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3302731/ https://www.ncbi.nlm.nih.gov/pubmed/22298429 http://dx.doi.org/10.1091/mbc.E11-09-0767 |
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