Cargando…

The coupling between sister kinetochore directional instability and oscillations in centromere stretch in metaphase PtK1 cells

Kinetochores bound to kinetochore microtubules (kMTs) exhibit directional instability in mammalian and other mitotic vertebrate cells, oscillating between poleward (P) and away-from-the-pole (AP) movements. These oscillations are coupled to changes in length of kMTs in a way that maintains a net str...

Descripción completa

Detalles Bibliográficos
Autores principales: Wan, Xiaohu, Cimini, Daniela, Cameron, Lisa A., Salmon, E. D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3302731/
https://www.ncbi.nlm.nih.gov/pubmed/22298429
http://dx.doi.org/10.1091/mbc.E11-09-0767
_version_ 1782226664258797568
author Wan, Xiaohu
Cimini, Daniela
Cameron, Lisa A.
Salmon, E. D.
author_facet Wan, Xiaohu
Cimini, Daniela
Cameron, Lisa A.
Salmon, E. D.
author_sort Wan, Xiaohu
collection PubMed
description Kinetochores bound to kinetochore microtubules (kMTs) exhibit directional instability in mammalian and other mitotic vertebrate cells, oscillating between poleward (P) and away-from-the-pole (AP) movements. These oscillations are coupled to changes in length of kMTs in a way that maintains a net stretch of the centromere. To understand how sister kinetochore directional instability and kMT plus-end dynamic instability are coupled to oscillations in centromere stretch, we tracked at high resolution the positions of fluorescent kinetochores and their poles for oscillating chromosomes within spindles of metaphase PtK1 cells. We found that the kinetics of P and AP movement are nonlinear and different. By subtracting contributions from the poleward flux of kMTs, we found that maximum centromere stretch occurred when the leading kinetochore switched from depolymerization to polymerization, whereas minimum centromere stretch occurred on average 7 s after the initially trailing kinetochore switched from polymerization to depolymerization. These differences produce oscillations in centromere stretch at about twice the frequency of kinetochore directional instability and at about twice the frequency of centromere oscillations back and forth across the spindle equator.
format Online
Article
Text
id pubmed-3302731
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher The American Society for Cell Biology
record_format MEDLINE/PubMed
spelling pubmed-33027312012-05-30 The coupling between sister kinetochore directional instability and oscillations in centromere stretch in metaphase PtK1 cells Wan, Xiaohu Cimini, Daniela Cameron, Lisa A. Salmon, E. D. Mol Biol Cell Articles Kinetochores bound to kinetochore microtubules (kMTs) exhibit directional instability in mammalian and other mitotic vertebrate cells, oscillating between poleward (P) and away-from-the-pole (AP) movements. These oscillations are coupled to changes in length of kMTs in a way that maintains a net stretch of the centromere. To understand how sister kinetochore directional instability and kMT plus-end dynamic instability are coupled to oscillations in centromere stretch, we tracked at high resolution the positions of fluorescent kinetochores and their poles for oscillating chromosomes within spindles of metaphase PtK1 cells. We found that the kinetics of P and AP movement are nonlinear and different. By subtracting contributions from the poleward flux of kMTs, we found that maximum centromere stretch occurred when the leading kinetochore switched from depolymerization to polymerization, whereas minimum centromere stretch occurred on average 7 s after the initially trailing kinetochore switched from polymerization to depolymerization. These differences produce oscillations in centromere stretch at about twice the frequency of kinetochore directional instability and at about twice the frequency of centromere oscillations back and forth across the spindle equator. The American Society for Cell Biology 2012-03-15 /pmc/articles/PMC3302731/ /pubmed/22298429 http://dx.doi.org/10.1091/mbc.E11-09-0767 Text en © 2012 Wan et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Wan, Xiaohu
Cimini, Daniela
Cameron, Lisa A.
Salmon, E. D.
The coupling between sister kinetochore directional instability and oscillations in centromere stretch in metaphase PtK1 cells
title The coupling between sister kinetochore directional instability and oscillations in centromere stretch in metaphase PtK1 cells
title_full The coupling between sister kinetochore directional instability and oscillations in centromere stretch in metaphase PtK1 cells
title_fullStr The coupling between sister kinetochore directional instability and oscillations in centromere stretch in metaphase PtK1 cells
title_full_unstemmed The coupling between sister kinetochore directional instability and oscillations in centromere stretch in metaphase PtK1 cells
title_short The coupling between sister kinetochore directional instability and oscillations in centromere stretch in metaphase PtK1 cells
title_sort coupling between sister kinetochore directional instability and oscillations in centromere stretch in metaphase ptk1 cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3302731/
https://www.ncbi.nlm.nih.gov/pubmed/22298429
http://dx.doi.org/10.1091/mbc.E11-09-0767
work_keys_str_mv AT wanxiaohu thecouplingbetweensisterkinetochoredirectionalinstabilityandoscillationsincentromerestretchinmetaphaseptk1cells
AT ciminidaniela thecouplingbetweensisterkinetochoredirectionalinstabilityandoscillationsincentromerestretchinmetaphaseptk1cells
AT cameronlisaa thecouplingbetweensisterkinetochoredirectionalinstabilityandoscillationsincentromerestretchinmetaphaseptk1cells
AT salmoned thecouplingbetweensisterkinetochoredirectionalinstabilityandoscillationsincentromerestretchinmetaphaseptk1cells
AT wanxiaohu couplingbetweensisterkinetochoredirectionalinstabilityandoscillationsincentromerestretchinmetaphaseptk1cells
AT ciminidaniela couplingbetweensisterkinetochoredirectionalinstabilityandoscillationsincentromerestretchinmetaphaseptk1cells
AT cameronlisaa couplingbetweensisterkinetochoredirectionalinstabilityandoscillationsincentromerestretchinmetaphaseptk1cells
AT salmoned couplingbetweensisterkinetochoredirectionalinstabilityandoscillationsincentromerestretchinmetaphaseptk1cells