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Immune Responses in Pigs Vaccinated with Adjuvanted and Non-Adjuvanted A(H1N1)pdm/09 Influenza Vaccines Used in Human Immunization Programmes
Following the emergence and global spread of a novel H1N1 influenza virus in 2009, two A(H1N1)pdm/09 influenza vaccines produced from the A/California/07/09 H1N1 strain were selected and used for the national immunisation programme in the United Kingdom: an adjuvanted split virion vaccine and a non-...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3302873/ https://www.ncbi.nlm.nih.gov/pubmed/22427834 http://dx.doi.org/10.1371/journal.pone.0032400 |
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author | Lefevre, Eric A. Carr, B. Veronica Inman, Charlotte F. Prentice, Helen Brown, Ian H. Brookes, Sharon M. Garcon, Fanny Hill, Michelle L. Iqbal, Munir Elderfield, Ruth A. Barclay, Wendy S. Gubbins, Simon Bailey, Mick Charleston, Bryan |
author_facet | Lefevre, Eric A. Carr, B. Veronica Inman, Charlotte F. Prentice, Helen Brown, Ian H. Brookes, Sharon M. Garcon, Fanny Hill, Michelle L. Iqbal, Munir Elderfield, Ruth A. Barclay, Wendy S. Gubbins, Simon Bailey, Mick Charleston, Bryan |
author_sort | Lefevre, Eric A. |
collection | PubMed |
description | Following the emergence and global spread of a novel H1N1 influenza virus in 2009, two A(H1N1)pdm/09 influenza vaccines produced from the A/California/07/09 H1N1 strain were selected and used for the national immunisation programme in the United Kingdom: an adjuvanted split virion vaccine and a non-adjuvanted whole virion vaccine. In this study, we assessed the immune responses generated in inbred large white pigs (Babraham line) following vaccination with these vaccines and after challenge with A(H1N1)pdm/09 virus three months post-vaccination. Both vaccines elicited strong antibody responses, which included high levels of influenza-specific IgG1 and haemagglutination inhibition titres to H1 virus. Immunisation with the adjuvanted split vaccine induced significantly higher interferon gamma production, increased frequency of interferon gamma-producing cells and proliferation of CD4(−)CD8(+) (cytotoxic) and CD4(+)CD8(+) (helper) T cells, after in vitro re-stimulation. Despite significant differences in the magnitude and breadth of immune responses in the two vaccinated and mock treated groups, similar quantities of viral RNA were detected from the nasal cavity in all pigs after live virus challenge. The present study provides support for the use of the pig as a valid experimental model for influenza infections in humans, including the assessment of protective efficacy of therapeutic interventions. |
format | Online Article Text |
id | pubmed-3302873 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-33028732012-03-16 Immune Responses in Pigs Vaccinated with Adjuvanted and Non-Adjuvanted A(H1N1)pdm/09 Influenza Vaccines Used in Human Immunization Programmes Lefevre, Eric A. Carr, B. Veronica Inman, Charlotte F. Prentice, Helen Brown, Ian H. Brookes, Sharon M. Garcon, Fanny Hill, Michelle L. Iqbal, Munir Elderfield, Ruth A. Barclay, Wendy S. Gubbins, Simon Bailey, Mick Charleston, Bryan PLoS One Research Article Following the emergence and global spread of a novel H1N1 influenza virus in 2009, two A(H1N1)pdm/09 influenza vaccines produced from the A/California/07/09 H1N1 strain were selected and used for the national immunisation programme in the United Kingdom: an adjuvanted split virion vaccine and a non-adjuvanted whole virion vaccine. In this study, we assessed the immune responses generated in inbred large white pigs (Babraham line) following vaccination with these vaccines and after challenge with A(H1N1)pdm/09 virus three months post-vaccination. Both vaccines elicited strong antibody responses, which included high levels of influenza-specific IgG1 and haemagglutination inhibition titres to H1 virus. Immunisation with the adjuvanted split vaccine induced significantly higher interferon gamma production, increased frequency of interferon gamma-producing cells and proliferation of CD4(−)CD8(+) (cytotoxic) and CD4(+)CD8(+) (helper) T cells, after in vitro re-stimulation. Despite significant differences in the magnitude and breadth of immune responses in the two vaccinated and mock treated groups, similar quantities of viral RNA were detected from the nasal cavity in all pigs after live virus challenge. The present study provides support for the use of the pig as a valid experimental model for influenza infections in humans, including the assessment of protective efficacy of therapeutic interventions. Public Library of Science 2012-03-09 /pmc/articles/PMC3302873/ /pubmed/22427834 http://dx.doi.org/10.1371/journal.pone.0032400 Text en Lefevre et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Lefevre, Eric A. Carr, B. Veronica Inman, Charlotte F. Prentice, Helen Brown, Ian H. Brookes, Sharon M. Garcon, Fanny Hill, Michelle L. Iqbal, Munir Elderfield, Ruth A. Barclay, Wendy S. Gubbins, Simon Bailey, Mick Charleston, Bryan Immune Responses in Pigs Vaccinated with Adjuvanted and Non-Adjuvanted A(H1N1)pdm/09 Influenza Vaccines Used in Human Immunization Programmes |
title | Immune Responses in Pigs Vaccinated with Adjuvanted and Non-Adjuvanted A(H1N1)pdm/09 Influenza Vaccines Used in Human Immunization Programmes |
title_full | Immune Responses in Pigs Vaccinated with Adjuvanted and Non-Adjuvanted A(H1N1)pdm/09 Influenza Vaccines Used in Human Immunization Programmes |
title_fullStr | Immune Responses in Pigs Vaccinated with Adjuvanted and Non-Adjuvanted A(H1N1)pdm/09 Influenza Vaccines Used in Human Immunization Programmes |
title_full_unstemmed | Immune Responses in Pigs Vaccinated with Adjuvanted and Non-Adjuvanted A(H1N1)pdm/09 Influenza Vaccines Used in Human Immunization Programmes |
title_short | Immune Responses in Pigs Vaccinated with Adjuvanted and Non-Adjuvanted A(H1N1)pdm/09 Influenza Vaccines Used in Human Immunization Programmes |
title_sort | immune responses in pigs vaccinated with adjuvanted and non-adjuvanted a(h1n1)pdm/09 influenza vaccines used in human immunization programmes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3302873/ https://www.ncbi.nlm.nih.gov/pubmed/22427834 http://dx.doi.org/10.1371/journal.pone.0032400 |
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