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Toxoplasma gondii Chromodomain Protein 1 Binds to Heterochromatin and Colocalises with Centromeres and Telomeres at the Nuclear Periphery

BACKGROUND: Apicomplexan parasites are responsible for some of the most deadly parasitic diseases afflicting humans, including malaria and toxoplasmosis. These obligate intracellular parasites exhibit a complex life cycle and a coordinated cell cycle-dependant expression program. Their cell division...

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Autores principales: Gissot, Mathieu, Walker, Robert, Delhaye, Stephane, Huot, Ludovic, Hot, David, Tomavo, Stanislas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3302879/
https://www.ncbi.nlm.nih.gov/pubmed/22427862
http://dx.doi.org/10.1371/journal.pone.0032671
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author Gissot, Mathieu
Walker, Robert
Delhaye, Stephane
Huot, Ludovic
Hot, David
Tomavo, Stanislas
author_facet Gissot, Mathieu
Walker, Robert
Delhaye, Stephane
Huot, Ludovic
Hot, David
Tomavo, Stanislas
author_sort Gissot, Mathieu
collection PubMed
description BACKGROUND: Apicomplexan parasites are responsible for some of the most deadly parasitic diseases afflicting humans, including malaria and toxoplasmosis. These obligate intracellular parasites exhibit a complex life cycle and a coordinated cell cycle-dependant expression program. Their cell division is a coordinated multistep process. How this complex mechanism is organised remains poorly understood. METHODS AND FINDINGS: In this study, we provide evidence for a link between heterochromatin, cell division and the compartmentalisation of the nucleus in Toxoplasma gondii. We characterised a T. gondii chromodomain containing protein (named TgChromo1) that specifically binds to heterochromatin. Using ChIP-on-chip on a genome-wide scale, we report TgChromo1 enrichment at the peri-centromeric chromatin. In addition, we demonstrate that TgChromo1 is cell-cycle regulated and co-localised with markers of the centrocone. Through the loci-specific FISH technique for T. gondii, we confirmed that TgChromo1 occupies the same nuclear localisation as the peri-centromeric sequences. CONCLUSION: We propose that TgChromo1 may play a role in the sequestration of chromosomes at the nuclear periphery and in the process of T. gondii cell division.
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spelling pubmed-33028792012-03-16 Toxoplasma gondii Chromodomain Protein 1 Binds to Heterochromatin and Colocalises with Centromeres and Telomeres at the Nuclear Periphery Gissot, Mathieu Walker, Robert Delhaye, Stephane Huot, Ludovic Hot, David Tomavo, Stanislas PLoS One Research Article BACKGROUND: Apicomplexan parasites are responsible for some of the most deadly parasitic diseases afflicting humans, including malaria and toxoplasmosis. These obligate intracellular parasites exhibit a complex life cycle and a coordinated cell cycle-dependant expression program. Their cell division is a coordinated multistep process. How this complex mechanism is organised remains poorly understood. METHODS AND FINDINGS: In this study, we provide evidence for a link between heterochromatin, cell division and the compartmentalisation of the nucleus in Toxoplasma gondii. We characterised a T. gondii chromodomain containing protein (named TgChromo1) that specifically binds to heterochromatin. Using ChIP-on-chip on a genome-wide scale, we report TgChromo1 enrichment at the peri-centromeric chromatin. In addition, we demonstrate that TgChromo1 is cell-cycle regulated and co-localised with markers of the centrocone. Through the loci-specific FISH technique for T. gondii, we confirmed that TgChromo1 occupies the same nuclear localisation as the peri-centromeric sequences. CONCLUSION: We propose that TgChromo1 may play a role in the sequestration of chromosomes at the nuclear periphery and in the process of T. gondii cell division. Public Library of Science 2012-03-09 /pmc/articles/PMC3302879/ /pubmed/22427862 http://dx.doi.org/10.1371/journal.pone.0032671 Text en Gissot et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Gissot, Mathieu
Walker, Robert
Delhaye, Stephane
Huot, Ludovic
Hot, David
Tomavo, Stanislas
Toxoplasma gondii Chromodomain Protein 1 Binds to Heterochromatin and Colocalises with Centromeres and Telomeres at the Nuclear Periphery
title Toxoplasma gondii Chromodomain Protein 1 Binds to Heterochromatin and Colocalises with Centromeres and Telomeres at the Nuclear Periphery
title_full Toxoplasma gondii Chromodomain Protein 1 Binds to Heterochromatin and Colocalises with Centromeres and Telomeres at the Nuclear Periphery
title_fullStr Toxoplasma gondii Chromodomain Protein 1 Binds to Heterochromatin and Colocalises with Centromeres and Telomeres at the Nuclear Periphery
title_full_unstemmed Toxoplasma gondii Chromodomain Protein 1 Binds to Heterochromatin and Colocalises with Centromeres and Telomeres at the Nuclear Periphery
title_short Toxoplasma gondii Chromodomain Protein 1 Binds to Heterochromatin and Colocalises with Centromeres and Telomeres at the Nuclear Periphery
title_sort toxoplasma gondii chromodomain protein 1 binds to heterochromatin and colocalises with centromeres and telomeres at the nuclear periphery
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3302879/
https://www.ncbi.nlm.nih.gov/pubmed/22427862
http://dx.doi.org/10.1371/journal.pone.0032671
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