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Cloning and expression of new microRNAs from zebrafish

MicroRNAs (miRNAs) play an important role in development and regulate the expression of many animal genes by post-transcriptional gene silencing. Here we describe the cloning and expression of new miRNAs from zebrafish. By high-throughput sequencing of small-RNA cDNA libraries from 5-day-old zebrafi...

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Autores principales: Kloosterman, Wigard P., Steiner, Florian A., Berezikov, Eugene, de Bruijn, Ewart, van de Belt, Jose, Verheul, Mark, Cuppen, Edwin, Plasterk, Ronald H.A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3303176/
https://www.ncbi.nlm.nih.gov/pubmed/16698962
http://dx.doi.org/10.1093/nar/gkl278
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author Kloosterman, Wigard P.
Steiner, Florian A.
Berezikov, Eugene
de Bruijn, Ewart
van de Belt, Jose
Verheul, Mark
Cuppen, Edwin
Plasterk, Ronald H.A.
author_facet Kloosterman, Wigard P.
Steiner, Florian A.
Berezikov, Eugene
de Bruijn, Ewart
van de Belt, Jose
Verheul, Mark
Cuppen, Edwin
Plasterk, Ronald H.A.
author_sort Kloosterman, Wigard P.
collection PubMed
description MicroRNAs (miRNAs) play an important role in development and regulate the expression of many animal genes by post-transcriptional gene silencing. Here we describe the cloning and expression of new miRNAs from zebrafish. By high-throughput sequencing of small-RNA cDNA libraries from 5-day-old zebrafish larvae and adult zebrafish brain we found 139 known miRNAs and 66 new miRNAs. For 65 known miRNAs and for 11 new miRNAs we also cloned the miRNA star sequence. We analyzed the temporal and spatial expression patterns for 35 new miRNAs and for 32 known miRNAs in the zebrafish by whole mount in situ hybridization and northern blotting. Overall, 23 of the 35 new miRNAs and 30 of the 32 known miRNAs could be detected. We found that most miRNAs were expressed during later stages of development. Some were expressed ubiquitously, but many of the miRNAs were expressed in a tissue-specific manner. Most newly discovered miRNAs have low expression levels and are less conserved in other vertebrate species. Our cloning and expression analysis indicates that most abundant and conserved miRNAs in zebrafish are now known.
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spelling pubmed-33031762012-03-14 Cloning and expression of new microRNAs from zebrafish Kloosterman, Wigard P. Steiner, Florian A. Berezikov, Eugene de Bruijn, Ewart van de Belt, Jose Verheul, Mark Cuppen, Edwin Plasterk, Ronald H.A. Nucleic Acids Res Article MicroRNAs (miRNAs) play an important role in development and regulate the expression of many animal genes by post-transcriptional gene silencing. Here we describe the cloning and expression of new miRNAs from zebrafish. By high-throughput sequencing of small-RNA cDNA libraries from 5-day-old zebrafish larvae and adult zebrafish brain we found 139 known miRNAs and 66 new miRNAs. For 65 known miRNAs and for 11 new miRNAs we also cloned the miRNA star sequence. We analyzed the temporal and spatial expression patterns for 35 new miRNAs and for 32 known miRNAs in the zebrafish by whole mount in situ hybridization and northern blotting. Overall, 23 of the 35 new miRNAs and 30 of the 32 known miRNAs could be detected. We found that most miRNAs were expressed during later stages of development. Some were expressed ubiquitously, but many of the miRNAs were expressed in a tissue-specific manner. Most newly discovered miRNAs have low expression levels and are less conserved in other vertebrate species. Our cloning and expression analysis indicates that most abundant and conserved miRNAs in zebrafish are now known. Oxford University Press 2006 /pmc/articles/PMC3303176/ /pubmed/16698962 http://dx.doi.org/10.1093/nar/gkl278 Text en © The Author 2006. Published by Oxford University Press. All rights reserved The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org
spellingShingle Article
Kloosterman, Wigard P.
Steiner, Florian A.
Berezikov, Eugene
de Bruijn, Ewart
van de Belt, Jose
Verheul, Mark
Cuppen, Edwin
Plasterk, Ronald H.A.
Cloning and expression of new microRNAs from zebrafish
title Cloning and expression of new microRNAs from zebrafish
title_full Cloning and expression of new microRNAs from zebrafish
title_fullStr Cloning and expression of new microRNAs from zebrafish
title_full_unstemmed Cloning and expression of new microRNAs from zebrafish
title_short Cloning and expression of new microRNAs from zebrafish
title_sort cloning and expression of new micrornas from zebrafish
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3303176/
https://www.ncbi.nlm.nih.gov/pubmed/16698962
http://dx.doi.org/10.1093/nar/gkl278
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