Radial Sizing of Lipid Nanotubes Using Membrane Displacement Analysis

[Image: see text] We report a novel method for the measurement of lipid nanotube radii. Membrane translocation is monitored between two nanotube-connected vesicles, during the expansion of a receiving vesicle, by observing a photobleached region of the nanotube. We elucidate nanotube radii, extracte...

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Detalles Bibliográficos
Autores principales: Stepanyants, Natalia, Jeffries, Gavin D. M., Orwar, Owe, Jesorka, Aldo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2012
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3303199/
https://www.ncbi.nlm.nih.gov/pubmed/22313341
http://dx.doi.org/10.1021/nl203983e
Descripción
Sumario:[Image: see text] We report a novel method for the measurement of lipid nanotube radii. Membrane translocation is monitored between two nanotube-connected vesicles, during the expansion of a receiving vesicle, by observing a photobleached region of the nanotube. We elucidate nanotube radii, extracted from SPE vesicles, enabling quantification of membrane composition and lamellarity. Variances of nanotube radii were measured, showing a growth of 40–56 nm, upon increasing cholesterol content from 0 to 20%.

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