Radial Sizing of Lipid Nanotubes Using Membrane Displacement Analysis

[Image: see text] We report a novel method for the measurement of lipid nanotube radii. Membrane translocation is monitored between two nanotube-connected vesicles, during the expansion of a receiving vesicle, by observing a photobleached region of the nanotube. We elucidate nanotube radii, extracte...

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Detalles Bibliográficos
Autores principales: Stepanyants, Natalia, Jeffries, Gavin D. M., Orwar, Owe, Jesorka, Aldo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2012
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3303199/
https://www.ncbi.nlm.nih.gov/pubmed/22313341
http://dx.doi.org/10.1021/nl203983e
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author Stepanyants, Natalia
Jeffries, Gavin D. M.
Orwar, Owe
Jesorka, Aldo
author_facet Stepanyants, Natalia
Jeffries, Gavin D. M.
Orwar, Owe
Jesorka, Aldo
author_sort Stepanyants, Natalia
collection PubMed
description [Image: see text] We report a novel method for the measurement of lipid nanotube radii. Membrane translocation is monitored between two nanotube-connected vesicles, during the expansion of a receiving vesicle, by observing a photobleached region of the nanotube. We elucidate nanotube radii, extracted from SPE vesicles, enabling quantification of membrane composition and lamellarity. Variances of nanotube radii were measured, showing a growth of 40–56 nm, upon increasing cholesterol content from 0 to 20%.
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spelling pubmed-33031992012-03-14 Radial Sizing of Lipid Nanotubes Using Membrane Displacement Analysis Stepanyants, Natalia Jeffries, Gavin D. M. Orwar, Owe Jesorka, Aldo Nano Lett [Image: see text] We report a novel method for the measurement of lipid nanotube radii. Membrane translocation is monitored between two nanotube-connected vesicles, during the expansion of a receiving vesicle, by observing a photobleached region of the nanotube. We elucidate nanotube radii, extracted from SPE vesicles, enabling quantification of membrane composition and lamellarity. Variances of nanotube radii were measured, showing a growth of 40–56 nm, upon increasing cholesterol content from 0 to 20%. American Chemical Society 2012-02-07 2012-03-14 /pmc/articles/PMC3303199/ /pubmed/22313341 http://dx.doi.org/10.1021/nl203983e Text en Copyright © 2012 American Chemical Society http://pubs.acs.org This is an open-access article distributed under the ACS AuthorChoice Terms & Conditions. Any use of this article, must conform to the terms of that license which are available at http://pubs.acs.org.
spellingShingle Stepanyants, Natalia
Jeffries, Gavin D. M.
Orwar, Owe
Jesorka, Aldo
Radial Sizing of Lipid Nanotubes Using Membrane Displacement Analysis
title Radial Sizing of Lipid Nanotubes Using Membrane Displacement Analysis
title_full Radial Sizing of Lipid Nanotubes Using Membrane Displacement Analysis
title_fullStr Radial Sizing of Lipid Nanotubes Using Membrane Displacement Analysis
title_full_unstemmed Radial Sizing of Lipid Nanotubes Using Membrane Displacement Analysis
title_short Radial Sizing of Lipid Nanotubes Using Membrane Displacement Analysis
title_sort radial sizing of lipid nanotubes using membrane displacement analysis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3303199/
https://www.ncbi.nlm.nih.gov/pubmed/22313341
http://dx.doi.org/10.1021/nl203983e
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