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Advances in BAC-Based Physical Mapping and Map Integration Strategies in Plants

In the advent of next-generation sequencing (NGS) platforms, map-based sequencing strategy has been recently suppressed being too expensive and laborious. The detailed studies on NGS drafts alone indicated these assemblies remain far from gold standard reference quality, especially when applied on c...

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Autores principales: Ariyadasa, Ruvini, Stein, Nils
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3303678/
https://www.ncbi.nlm.nih.gov/pubmed/22500080
http://dx.doi.org/10.1155/2012/184854
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author Ariyadasa, Ruvini
Stein, Nils
author_facet Ariyadasa, Ruvini
Stein, Nils
author_sort Ariyadasa, Ruvini
collection PubMed
description In the advent of next-generation sequencing (NGS) platforms, map-based sequencing strategy has been recently suppressed being too expensive and laborious. The detailed studies on NGS drafts alone indicated these assemblies remain far from gold standard reference quality, especially when applied on complex genomes. In this context the conventional BAC-based physical mapping has been identified as an important intermediate layer in current hybrid sequencing strategy. BAC-based physical map construction and its integration with high-density genetic maps have benefited from NGS and high-throughput array platforms. This paper addresses the current advancements of BAC-based physical mapping and high-throughput map integration strategies to obtain densely anchored well-ordered physical maps. The resulted maps are of immediate utility while providing a template to harness the maximum benefits of the current NGS platforms.
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spelling pubmed-33036782012-04-12 Advances in BAC-Based Physical Mapping and Map Integration Strategies in Plants Ariyadasa, Ruvini Stein, Nils J Biomed Biotechnol Review Article In the advent of next-generation sequencing (NGS) platforms, map-based sequencing strategy has been recently suppressed being too expensive and laborious. The detailed studies on NGS drafts alone indicated these assemblies remain far from gold standard reference quality, especially when applied on complex genomes. In this context the conventional BAC-based physical mapping has been identified as an important intermediate layer in current hybrid sequencing strategy. BAC-based physical map construction and its integration with high-density genetic maps have benefited from NGS and high-throughput array platforms. This paper addresses the current advancements of BAC-based physical mapping and high-throughput map integration strategies to obtain densely anchored well-ordered physical maps. The resulted maps are of immediate utility while providing a template to harness the maximum benefits of the current NGS platforms. Hindawi Publishing Corporation 2012 2012-02-27 /pmc/articles/PMC3303678/ /pubmed/22500080 http://dx.doi.org/10.1155/2012/184854 Text en Copyright © 2012 R. Ariyadasa and N. Stein. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Ariyadasa, Ruvini
Stein, Nils
Advances in BAC-Based Physical Mapping and Map Integration Strategies in Plants
title Advances in BAC-Based Physical Mapping and Map Integration Strategies in Plants
title_full Advances in BAC-Based Physical Mapping and Map Integration Strategies in Plants
title_fullStr Advances in BAC-Based Physical Mapping and Map Integration Strategies in Plants
title_full_unstemmed Advances in BAC-Based Physical Mapping and Map Integration Strategies in Plants
title_short Advances in BAC-Based Physical Mapping and Map Integration Strategies in Plants
title_sort advances in bac-based physical mapping and map integration strategies in plants
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3303678/
https://www.ncbi.nlm.nih.gov/pubmed/22500080
http://dx.doi.org/10.1155/2012/184854
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