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Activations of Both Extrinsic and Intrinsic Pathways in HCT 116 Human Colorectal Cancer Cells Contribute to Apoptosis through p53-Mediated ATM/Fas Signaling by Emilia sonchifolia Extract, a Folklore Medicinal Plant
Emilia sonchifolia (L.) DC (Compositae), an herbaceous plant found in Taiwan and India, is used as folk medicine. The clinical applications include inflammation, rheumatism, cough, cuts fever, dysentery, analgesic, and antibacteria. The activities of Emilia sonchifolia extract (ESE) on colorectal ca...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3303801/ https://www.ncbi.nlm.nih.gov/pubmed/22474491 http://dx.doi.org/10.1155/2012/178178 |
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author | Lan, Yu-Hsuan Chiang, Jo-Hua Huang, Wen-Wen Lu, Chi-Cheng Chung, Jing-Gung Wu, Tian-Shung Jhan, Jia-Hua Lin, Kuei-Li Pai, Shu-Jen Chiu, Yu-Jen Tsuzuki, Minoru Yang, Jai-Sing |
author_facet | Lan, Yu-Hsuan Chiang, Jo-Hua Huang, Wen-Wen Lu, Chi-Cheng Chung, Jing-Gung Wu, Tian-Shung Jhan, Jia-Hua Lin, Kuei-Li Pai, Shu-Jen Chiu, Yu-Jen Tsuzuki, Minoru Yang, Jai-Sing |
author_sort | Lan, Yu-Hsuan |
collection | PubMed |
description | Emilia sonchifolia (L.) DC (Compositae), an herbaceous plant found in Taiwan and India, is used as folk medicine. The clinical applications include inflammation, rheumatism, cough, cuts fever, dysentery, analgesic, and antibacteria. The activities of Emilia sonchifolia extract (ESE) on colorectal cancer cell death have not been fully investigated. The purpose of this study explored the induction of apoptosis and its molecular mechanisms in ESE-treated HCT 116 human colorectal cancer cells in vitro. The methanolic ESE was characterized, and γ-humulene was formed as the major constituent (63.86%). ESE induced cell growth inhibition in a concentration- and time-dependent response by MTT assay. Apoptotic cells (DNA fragmentation, an apoptotic catachrestic) were found after ESE treatment by TUNEL assay and DNA gel electrophoresis. Alternatively, ESE stimulated the activities of caspase-3, -8, and -9 and their specific caspase inhibitors protected against ESE-induced cytotoxicity. ESE promoted the mitochondria-dependent and death-receptor-associated protein levels. Also, ESE increased ROS production and upregulated the levels of ATM, p53, and Fas in HCT 116 cells. Strikingly, p53 siRNA reversed ESE-reduced viability involved in p53-mediated ATM/Fas signaling in HCT 116 cells. In summary, our result is the first report suggesting that ESE may be potentially efficacious in the treatment of colorectal cancer. |
format | Online Article Text |
id | pubmed-3303801 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-33038012012-04-03 Activations of Both Extrinsic and Intrinsic Pathways in HCT 116 Human Colorectal Cancer Cells Contribute to Apoptosis through p53-Mediated ATM/Fas Signaling by Emilia sonchifolia Extract, a Folklore Medicinal Plant Lan, Yu-Hsuan Chiang, Jo-Hua Huang, Wen-Wen Lu, Chi-Cheng Chung, Jing-Gung Wu, Tian-Shung Jhan, Jia-Hua Lin, Kuei-Li Pai, Shu-Jen Chiu, Yu-Jen Tsuzuki, Minoru Yang, Jai-Sing Evid Based Complement Alternat Med Research Article Emilia sonchifolia (L.) DC (Compositae), an herbaceous plant found in Taiwan and India, is used as folk medicine. The clinical applications include inflammation, rheumatism, cough, cuts fever, dysentery, analgesic, and antibacteria. The activities of Emilia sonchifolia extract (ESE) on colorectal cancer cell death have not been fully investigated. The purpose of this study explored the induction of apoptosis and its molecular mechanisms in ESE-treated HCT 116 human colorectal cancer cells in vitro. The methanolic ESE was characterized, and γ-humulene was formed as the major constituent (63.86%). ESE induced cell growth inhibition in a concentration- and time-dependent response by MTT assay. Apoptotic cells (DNA fragmentation, an apoptotic catachrestic) were found after ESE treatment by TUNEL assay and DNA gel electrophoresis. Alternatively, ESE stimulated the activities of caspase-3, -8, and -9 and their specific caspase inhibitors protected against ESE-induced cytotoxicity. ESE promoted the mitochondria-dependent and death-receptor-associated protein levels. Also, ESE increased ROS production and upregulated the levels of ATM, p53, and Fas in HCT 116 cells. Strikingly, p53 siRNA reversed ESE-reduced viability involved in p53-mediated ATM/Fas signaling in HCT 116 cells. In summary, our result is the first report suggesting that ESE may be potentially efficacious in the treatment of colorectal cancer. Hindawi Publishing Corporation 2012 2012-02-28 /pmc/articles/PMC3303801/ /pubmed/22474491 http://dx.doi.org/10.1155/2012/178178 Text en Copyright © 2012 Yu-Hsuan Lan et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Lan, Yu-Hsuan Chiang, Jo-Hua Huang, Wen-Wen Lu, Chi-Cheng Chung, Jing-Gung Wu, Tian-Shung Jhan, Jia-Hua Lin, Kuei-Li Pai, Shu-Jen Chiu, Yu-Jen Tsuzuki, Minoru Yang, Jai-Sing Activations of Both Extrinsic and Intrinsic Pathways in HCT 116 Human Colorectal Cancer Cells Contribute to Apoptosis through p53-Mediated ATM/Fas Signaling by Emilia sonchifolia Extract, a Folklore Medicinal Plant |
title | Activations of Both Extrinsic and Intrinsic Pathways in HCT 116 Human Colorectal Cancer Cells Contribute to Apoptosis through p53-Mediated ATM/Fas Signaling by Emilia sonchifolia Extract, a Folklore Medicinal Plant |
title_full | Activations of Both Extrinsic and Intrinsic Pathways in HCT 116 Human Colorectal Cancer Cells Contribute to Apoptosis through p53-Mediated ATM/Fas Signaling by Emilia sonchifolia Extract, a Folklore Medicinal Plant |
title_fullStr | Activations of Both Extrinsic and Intrinsic Pathways in HCT 116 Human Colorectal Cancer Cells Contribute to Apoptosis through p53-Mediated ATM/Fas Signaling by Emilia sonchifolia Extract, a Folklore Medicinal Plant |
title_full_unstemmed | Activations of Both Extrinsic and Intrinsic Pathways in HCT 116 Human Colorectal Cancer Cells Contribute to Apoptosis through p53-Mediated ATM/Fas Signaling by Emilia sonchifolia Extract, a Folklore Medicinal Plant |
title_short | Activations of Both Extrinsic and Intrinsic Pathways in HCT 116 Human Colorectal Cancer Cells Contribute to Apoptosis through p53-Mediated ATM/Fas Signaling by Emilia sonchifolia Extract, a Folklore Medicinal Plant |
title_sort | activations of both extrinsic and intrinsic pathways in hct 116 human colorectal cancer cells contribute to apoptosis through p53-mediated atm/fas signaling by emilia sonchifolia extract, a folklore medicinal plant |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3303801/ https://www.ncbi.nlm.nih.gov/pubmed/22474491 http://dx.doi.org/10.1155/2012/178178 |
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