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Potential Stemness of Frozen-Thawed Testicular Biopsies without Sperm in Infertile Men Included into the In Vitro Fertilization Programme

We describe the potential stemness of a small amount of frozen-thawed testicular tissue without sperm obtained by biopsy from six patients undergoing assisted reproductive treatment. The patients were diagnosed with Sertoli Cell-Only Syndrome alone or combined with maturation arrest. Trying to provi...

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Autores principales: Stimpfel, Martin, Skutella, Thomas, Kubista, Mikael, Malicev, Elvira, Conrad, Sabine, Virant-Klun, Irma
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3303891/
https://www.ncbi.nlm.nih.gov/pubmed/22431916
http://dx.doi.org/10.1155/2012/291038
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author Stimpfel, Martin
Skutella, Thomas
Kubista, Mikael
Malicev, Elvira
Conrad, Sabine
Virant-Klun, Irma
author_facet Stimpfel, Martin
Skutella, Thomas
Kubista, Mikael
Malicev, Elvira
Conrad, Sabine
Virant-Klun, Irma
author_sort Stimpfel, Martin
collection PubMed
description We describe the potential stemness of a small amount of frozen-thawed testicular tissue without sperm obtained by biopsy from six patients undergoing assisted reproductive treatment. The patients were diagnosed with Sertoli Cell-Only Syndrome alone or combined with maturation arrest. Trying to provide the natural stem cell niche for cultured stem cells, all isolated cells from enzymatically degraded biopsies where cultured together in different culture media and the presence of putative mesenchymal and putative pluripotent ES-like stem cells was indicated using different methods. High throughput real-time quantitative PCR followed by multivariate analysis revealed the formation of distinct cell clusters reflecting high degree of similarity and some of these cell clusters expressed the genes characteristic for pluripotent stem cells. In the presence of the follicular fluid, prepared as serum, putative testicular stem cells showed a certain degree of plasticity, and spontaneously differentiated into adipose-like and neuronal-like cells. Additionally, using differentiation protocols putative testicular stem cells were differentiated into neuronal- and pancreatic-like cells. This study shows that in assisted reproduction programmes, testicular tissue with no sperm might be an important source of stem cells, although it is discarded in daily medical practice; this requires further research.
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spelling pubmed-33038912012-03-19 Potential Stemness of Frozen-Thawed Testicular Biopsies without Sperm in Infertile Men Included into the In Vitro Fertilization Programme Stimpfel, Martin Skutella, Thomas Kubista, Mikael Malicev, Elvira Conrad, Sabine Virant-Klun, Irma J Biomed Biotechnol Research Article We describe the potential stemness of a small amount of frozen-thawed testicular tissue without sperm obtained by biopsy from six patients undergoing assisted reproductive treatment. The patients were diagnosed with Sertoli Cell-Only Syndrome alone or combined with maturation arrest. Trying to provide the natural stem cell niche for cultured stem cells, all isolated cells from enzymatically degraded biopsies where cultured together in different culture media and the presence of putative mesenchymal and putative pluripotent ES-like stem cells was indicated using different methods. High throughput real-time quantitative PCR followed by multivariate analysis revealed the formation of distinct cell clusters reflecting high degree of similarity and some of these cell clusters expressed the genes characteristic for pluripotent stem cells. In the presence of the follicular fluid, prepared as serum, putative testicular stem cells showed a certain degree of plasticity, and spontaneously differentiated into adipose-like and neuronal-like cells. Additionally, using differentiation protocols putative testicular stem cells were differentiated into neuronal- and pancreatic-like cells. This study shows that in assisted reproduction programmes, testicular tissue with no sperm might be an important source of stem cells, although it is discarded in daily medical practice; this requires further research. Hindawi Publishing Corporation 2012 2012-02-08 /pmc/articles/PMC3303891/ /pubmed/22431916 http://dx.doi.org/10.1155/2012/291038 Text en Copyright © 2012 Martin Stimpfel et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Stimpfel, Martin
Skutella, Thomas
Kubista, Mikael
Malicev, Elvira
Conrad, Sabine
Virant-Klun, Irma
Potential Stemness of Frozen-Thawed Testicular Biopsies without Sperm in Infertile Men Included into the In Vitro Fertilization Programme
title Potential Stemness of Frozen-Thawed Testicular Biopsies without Sperm in Infertile Men Included into the In Vitro Fertilization Programme
title_full Potential Stemness of Frozen-Thawed Testicular Biopsies without Sperm in Infertile Men Included into the In Vitro Fertilization Programme
title_fullStr Potential Stemness of Frozen-Thawed Testicular Biopsies without Sperm in Infertile Men Included into the In Vitro Fertilization Programme
title_full_unstemmed Potential Stemness of Frozen-Thawed Testicular Biopsies without Sperm in Infertile Men Included into the In Vitro Fertilization Programme
title_short Potential Stemness of Frozen-Thawed Testicular Biopsies without Sperm in Infertile Men Included into the In Vitro Fertilization Programme
title_sort potential stemness of frozen-thawed testicular biopsies without sperm in infertile men included into the in vitro fertilization programme
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3303891/
https://www.ncbi.nlm.nih.gov/pubmed/22431916
http://dx.doi.org/10.1155/2012/291038
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