Dual-objective STORM reveals three-dimensional filament organization in the actin cytoskeleton

By combining astigmatism imaging with a dual-objective scheme, we improved the image resolution of stochastic optical reconstruction microscopy (STORM) and obtained <10 nm lateral resolution and <20 nm axial resolution when imaging biological specimens. Using this approach, we resolved individ...

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Detalles Bibliográficos
Autores principales: Xu, Ke, Babcock, Hazen P., Zhuang, Xiaowei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3304438/
https://www.ncbi.nlm.nih.gov/pubmed/22231642
http://dx.doi.org/10.1038/nmeth.1841
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author Xu, Ke
Babcock, Hazen P.
Zhuang, Xiaowei
author_facet Xu, Ke
Babcock, Hazen P.
Zhuang, Xiaowei
author_sort Xu, Ke
collection PubMed
description By combining astigmatism imaging with a dual-objective scheme, we improved the image resolution of stochastic optical reconstruction microscopy (STORM) and obtained <10 nm lateral resolution and <20 nm axial resolution when imaging biological specimens. Using this approach, we resolved individual actin filaments in cells and revealed three-dimensional ultrastructure of the actin cytoskeleton. We observed two vertically separated layers of actin networks with distinct structural organizations in sheet-like cell protrusions.
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spelling pubmed-33044382012-08-01 Dual-objective STORM reveals three-dimensional filament organization in the actin cytoskeleton Xu, Ke Babcock, Hazen P. Zhuang, Xiaowei Nat Methods Article By combining astigmatism imaging with a dual-objective scheme, we improved the image resolution of stochastic optical reconstruction microscopy (STORM) and obtained <10 nm lateral resolution and <20 nm axial resolution when imaging biological specimens. Using this approach, we resolved individual actin filaments in cells and revealed three-dimensional ultrastructure of the actin cytoskeleton. We observed two vertically separated layers of actin networks with distinct structural organizations in sheet-like cell protrusions. 2012-01-08 /pmc/articles/PMC3304438/ /pubmed/22231642 http://dx.doi.org/10.1038/nmeth.1841 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Xu, Ke
Babcock, Hazen P.
Zhuang, Xiaowei
Dual-objective STORM reveals three-dimensional filament organization in the actin cytoskeleton
title Dual-objective STORM reveals three-dimensional filament organization in the actin cytoskeleton
title_full Dual-objective STORM reveals three-dimensional filament organization in the actin cytoskeleton
title_fullStr Dual-objective STORM reveals three-dimensional filament organization in the actin cytoskeleton
title_full_unstemmed Dual-objective STORM reveals three-dimensional filament organization in the actin cytoskeleton
title_short Dual-objective STORM reveals three-dimensional filament organization in the actin cytoskeleton
title_sort dual-objective storm reveals three-dimensional filament organization in the actin cytoskeleton
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3304438/
https://www.ncbi.nlm.nih.gov/pubmed/22231642
http://dx.doi.org/10.1038/nmeth.1841
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AT babcockhazenp dualobjectivestormrevealsthreedimensionalfilamentorganizationintheactincytoskeleton
AT zhuangxiaowei dualobjectivestormrevealsthreedimensionalfilamentorganizationintheactincytoskeleton

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