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Dual-objective STORM reveals three-dimensional filament organization in the actin cytoskeleton
By combining astigmatism imaging with a dual-objective scheme, we improved the image resolution of stochastic optical reconstruction microscopy (STORM) and obtained <10 nm lateral resolution and <20 nm axial resolution when imaging biological specimens. Using this approach, we resolved individ...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3304438/ https://www.ncbi.nlm.nih.gov/pubmed/22231642 http://dx.doi.org/10.1038/nmeth.1841 |