Cargando…

Detection of Hepatitis B virus in serum and liver of chickens

Hepatitis B virus (HBV) is one of the most important human pathogens. Its existence in food animals could present a significant threat to public health. The objective of this study was to determine if HBV is present in serum and liver of chickens. A total of 129 serum samples from broiler chickens w...

Descripción completa

Detalles Bibliográficos
Autores principales: Tian, Jijing, Xia, Kangkang, She, Ruiping, Li, Wengui, Ding, Ye, Wang, Jiande, Chen, Mingyong, Yin, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3306199/
https://www.ncbi.nlm.nih.gov/pubmed/22217003
http://dx.doi.org/10.1186/1743-422X-9-2
_version_ 1782227192224153600
author Tian, Jijing
Xia, Kangkang
She, Ruiping
Li, Wengui
Ding, Ye
Wang, Jiande
Chen, Mingyong
Yin, Jun
author_facet Tian, Jijing
Xia, Kangkang
She, Ruiping
Li, Wengui
Ding, Ye
Wang, Jiande
Chen, Mingyong
Yin, Jun
author_sort Tian, Jijing
collection PubMed
description Hepatitis B virus (HBV) is one of the most important human pathogens. Its existence in food animals could present a significant threat to public health. The objective of this study was to determine if HBV is present in serum and liver of chickens. A total of 129 serum samples from broiler chickens were collected for the detection of HBV antigens and antibodies, and 193 liver samples were tested for HBV DNA sequence by PCR and for the existence of HBV antigens by immunohistochemistry. The overall prevalence of HBsAg, anti-HBs, anti-HBc was 28.68%, 53.49%, 17.05%, respectively, whereas HBeAg, anti-HBe were barely detectable. Three serum samples were found to be positive for both HBsAg and HBeAg. Further analysis of these samples with transmission electron microscopy (TEM) revealed two morphologic particles with 20 nm and 40 nm in diameter, which were similar to small spherical and Danes particles of HBV. The viral DNA sequence identified in two of the chicken livers shared 92.2% of one known HBV strain and 97.9% nucleotide sequence of another HBV strain. Our results showed the existence of HBV in chickens. This would present a significant risk to people who work with live chickens or chicken products if HBV found in chicken could be confirmed to be the same as human HBV.
format Online
Article
Text
id pubmed-3306199
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-33061992012-03-17 Detection of Hepatitis B virus in serum and liver of chickens Tian, Jijing Xia, Kangkang She, Ruiping Li, Wengui Ding, Ye Wang, Jiande Chen, Mingyong Yin, Jun Virol J Research Hepatitis B virus (HBV) is one of the most important human pathogens. Its existence in food animals could present a significant threat to public health. The objective of this study was to determine if HBV is present in serum and liver of chickens. A total of 129 serum samples from broiler chickens were collected for the detection of HBV antigens and antibodies, and 193 liver samples were tested for HBV DNA sequence by PCR and for the existence of HBV antigens by immunohistochemistry. The overall prevalence of HBsAg, anti-HBs, anti-HBc was 28.68%, 53.49%, 17.05%, respectively, whereas HBeAg, anti-HBe were barely detectable. Three serum samples were found to be positive for both HBsAg and HBeAg. Further analysis of these samples with transmission electron microscopy (TEM) revealed two morphologic particles with 20 nm and 40 nm in diameter, which were similar to small spherical and Danes particles of HBV. The viral DNA sequence identified in two of the chicken livers shared 92.2% of one known HBV strain and 97.9% nucleotide sequence of another HBV strain. Our results showed the existence of HBV in chickens. This would present a significant risk to people who work with live chickens or chicken products if HBV found in chicken could be confirmed to be the same as human HBV. BioMed Central 2012-01-04 /pmc/articles/PMC3306199/ /pubmed/22217003 http://dx.doi.org/10.1186/1743-422X-9-2 Text en Copyright ©2011 Tian et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Tian, Jijing
Xia, Kangkang
She, Ruiping
Li, Wengui
Ding, Ye
Wang, Jiande
Chen, Mingyong
Yin, Jun
Detection of Hepatitis B virus in serum and liver of chickens
title Detection of Hepatitis B virus in serum and liver of chickens
title_full Detection of Hepatitis B virus in serum and liver of chickens
title_fullStr Detection of Hepatitis B virus in serum and liver of chickens
title_full_unstemmed Detection of Hepatitis B virus in serum and liver of chickens
title_short Detection of Hepatitis B virus in serum and liver of chickens
title_sort detection of hepatitis b virus in serum and liver of chickens
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3306199/
https://www.ncbi.nlm.nih.gov/pubmed/22217003
http://dx.doi.org/10.1186/1743-422X-9-2
work_keys_str_mv AT tianjijing detectionofhepatitisbvirusinserumandliverofchickens
AT xiakangkang detectionofhepatitisbvirusinserumandliverofchickens
AT sheruiping detectionofhepatitisbvirusinserumandliverofchickens
AT liwengui detectionofhepatitisbvirusinserumandliverofchickens
AT dingye detectionofhepatitisbvirusinserumandliverofchickens
AT wangjiande detectionofhepatitisbvirusinserumandliverofchickens
AT chenmingyong detectionofhepatitisbvirusinserumandliverofchickens
AT yinjun detectionofhepatitisbvirusinserumandliverofchickens