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Quantitative Proteome Profiling of C. burnetii under Tetracycline Stress Conditions

The recommended antibiotic regimen against Coxiella burnetii, the etiological agent of Q fever, is based on a semi-synthetic, second-generation tetracycline, doxycycline. Here, we report on the comparison of the proteomes of a C. burnetii reference strain either cultured under control conditions or...

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Autores principales: Vranakis, Iosif, De Bock, Pieter-Jan, Papadioti, Anastasia, Tselentis, Yannis, Gevaert, Kris, Tsiotis, Georgios, Psaroulaki, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3306420/
https://www.ncbi.nlm.nih.gov/pubmed/22438959
http://dx.doi.org/10.1371/journal.pone.0033599
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author Vranakis, Iosif
De Bock, Pieter-Jan
Papadioti, Anastasia
Tselentis, Yannis
Gevaert, Kris
Tsiotis, Georgios
Psaroulaki, Anna
author_facet Vranakis, Iosif
De Bock, Pieter-Jan
Papadioti, Anastasia
Tselentis, Yannis
Gevaert, Kris
Tsiotis, Georgios
Psaroulaki, Anna
author_sort Vranakis, Iosif
collection PubMed
description The recommended antibiotic regimen against Coxiella burnetii, the etiological agent of Q fever, is based on a semi-synthetic, second-generation tetracycline, doxycycline. Here, we report on the comparison of the proteomes of a C. burnetii reference strain either cultured under control conditions or under tetracycline stress conditions. Using the MS-driven combined fractional diagonal chromatography proteomics technique, out of the 531 proteins identified, 5 and 19 proteins were found significantly up- and down-regulated respectively, under tetracycline stress. Although the predicted cellular functions of these regulated proteins did not point to known tetracycline resistance mechanisms, our data clearly reveal the plasticity of the proteome of C. burnetii to battle tetracycline stress. Finally, we raise several plausible hypotheses that could further lead to more focused experiments on studying tetracycline resistance in C. burnetii and thus reduced treatment failures of Q fever.
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spelling pubmed-33064202012-03-21 Quantitative Proteome Profiling of C. burnetii under Tetracycline Stress Conditions Vranakis, Iosif De Bock, Pieter-Jan Papadioti, Anastasia Tselentis, Yannis Gevaert, Kris Tsiotis, Georgios Psaroulaki, Anna PLoS One Research Article The recommended antibiotic regimen against Coxiella burnetii, the etiological agent of Q fever, is based on a semi-synthetic, second-generation tetracycline, doxycycline. Here, we report on the comparison of the proteomes of a C. burnetii reference strain either cultured under control conditions or under tetracycline stress conditions. Using the MS-driven combined fractional diagonal chromatography proteomics technique, out of the 531 proteins identified, 5 and 19 proteins were found significantly up- and down-regulated respectively, under tetracycline stress. Although the predicted cellular functions of these regulated proteins did not point to known tetracycline resistance mechanisms, our data clearly reveal the plasticity of the proteome of C. burnetii to battle tetracycline stress. Finally, we raise several plausible hypotheses that could further lead to more focused experiments on studying tetracycline resistance in C. burnetii and thus reduced treatment failures of Q fever. Public Library of Science 2012-03-16 /pmc/articles/PMC3306420/ /pubmed/22438959 http://dx.doi.org/10.1371/journal.pone.0033599 Text en Vranakis et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Vranakis, Iosif
De Bock, Pieter-Jan
Papadioti, Anastasia
Tselentis, Yannis
Gevaert, Kris
Tsiotis, Georgios
Psaroulaki, Anna
Quantitative Proteome Profiling of C. burnetii under Tetracycline Stress Conditions
title Quantitative Proteome Profiling of C. burnetii under Tetracycline Stress Conditions
title_full Quantitative Proteome Profiling of C. burnetii under Tetracycline Stress Conditions
title_fullStr Quantitative Proteome Profiling of C. burnetii under Tetracycline Stress Conditions
title_full_unstemmed Quantitative Proteome Profiling of C. burnetii under Tetracycline Stress Conditions
title_short Quantitative Proteome Profiling of C. burnetii under Tetracycline Stress Conditions
title_sort quantitative proteome profiling of c. burnetii under tetracycline stress conditions
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3306420/
https://www.ncbi.nlm.nih.gov/pubmed/22438959
http://dx.doi.org/10.1371/journal.pone.0033599
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