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Involvement of the Ubiquitin-Proteasome System in the Formation of Experimental Postsurgical Peritoneal Adhesions

We investigated the Ubiquitin-Proteasome System (UPS), major nonlysosomal intracellular protein degradation system, in the genesis of experimental postsurgical peritoneal adhesions. We assayed the levels of UPS within the adhered tissue along with the development of peritoneal adhesions and used the...

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Autores principales: Di Filippo, Clara, Petronella, Pasquale, Freda, Fulvio, Scorzelli, Marco, Ferretti, Marco, Canonico, Sivestro, Rossi, Francesco, D'Amico, Michele
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3306991/
https://www.ncbi.nlm.nih.gov/pubmed/22496598
http://dx.doi.org/10.1155/2012/194723
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author Di Filippo, Clara
Petronella, Pasquale
Freda, Fulvio
Scorzelli, Marco
Ferretti, Marco
Canonico, Sivestro
Rossi, Francesco
D'Amico, Michele
author_facet Di Filippo, Clara
Petronella, Pasquale
Freda, Fulvio
Scorzelli, Marco
Ferretti, Marco
Canonico, Sivestro
Rossi, Francesco
D'Amico, Michele
author_sort Di Filippo, Clara
collection PubMed
description We investigated the Ubiquitin-Proteasome System (UPS), major nonlysosomal intracellular protein degradation system, in the genesis of experimental postsurgical peritoneal adhesions. We assayed the levels of UPS within the adhered tissue along with the development of peritoneal adhesions and used the specific UPS inhibitor bortezomib in order to assess the effect of the UPS blockade on the peritoneal adhesions. We found a number of severe postsurgical peritoneal adhesions at day 5 after surgery increasing until day 10. In the adhered tissue an increased values of ubiquitin and the 20S proteasome subunit, NFkB, IL-6, TNF-α and decreased values of IkB-beta were found. In contrast, bortezomib-treated rats showed a decreased number of peritoneal adhesions, decreased values of ubiquitin and the 20S proteasome, NFkB, IL-6, TNF-α, and increased levels of IkB-beta in the adhered peritoneal tissue. The UPS system, therefore, is primarily involved in the formation of post-surgical peritoneal adhesions in rats.
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spelling pubmed-33069912012-04-11 Involvement of the Ubiquitin-Proteasome System in the Formation of Experimental Postsurgical Peritoneal Adhesions Di Filippo, Clara Petronella, Pasquale Freda, Fulvio Scorzelli, Marco Ferretti, Marco Canonico, Sivestro Rossi, Francesco D'Amico, Michele Mediators Inflamm Research Article We investigated the Ubiquitin-Proteasome System (UPS), major nonlysosomal intracellular protein degradation system, in the genesis of experimental postsurgical peritoneal adhesions. We assayed the levels of UPS within the adhered tissue along with the development of peritoneal adhesions and used the specific UPS inhibitor bortezomib in order to assess the effect of the UPS blockade on the peritoneal adhesions. We found a number of severe postsurgical peritoneal adhesions at day 5 after surgery increasing until day 10. In the adhered tissue an increased values of ubiquitin and the 20S proteasome subunit, NFkB, IL-6, TNF-α and decreased values of IkB-beta were found. In contrast, bortezomib-treated rats showed a decreased number of peritoneal adhesions, decreased values of ubiquitin and the 20S proteasome, NFkB, IL-6, TNF-α, and increased levels of IkB-beta in the adhered peritoneal tissue. The UPS system, therefore, is primarily involved in the formation of post-surgical peritoneal adhesions in rats. Hindawi Publishing Corporation 2012 2012-02-14 /pmc/articles/PMC3306991/ /pubmed/22496598 http://dx.doi.org/10.1155/2012/194723 Text en Copyright © 2012 Clara Di Filippo et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Di Filippo, Clara
Petronella, Pasquale
Freda, Fulvio
Scorzelli, Marco
Ferretti, Marco
Canonico, Sivestro
Rossi, Francesco
D'Amico, Michele
Involvement of the Ubiquitin-Proteasome System in the Formation of Experimental Postsurgical Peritoneal Adhesions
title Involvement of the Ubiquitin-Proteasome System in the Formation of Experimental Postsurgical Peritoneal Adhesions
title_full Involvement of the Ubiquitin-Proteasome System in the Formation of Experimental Postsurgical Peritoneal Adhesions
title_fullStr Involvement of the Ubiquitin-Proteasome System in the Formation of Experimental Postsurgical Peritoneal Adhesions
title_full_unstemmed Involvement of the Ubiquitin-Proteasome System in the Formation of Experimental Postsurgical Peritoneal Adhesions
title_short Involvement of the Ubiquitin-Proteasome System in the Formation of Experimental Postsurgical Peritoneal Adhesions
title_sort involvement of the ubiquitin-proteasome system in the formation of experimental postsurgical peritoneal adhesions
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3306991/
https://www.ncbi.nlm.nih.gov/pubmed/22496598
http://dx.doi.org/10.1155/2012/194723
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