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Chromosomal abnormalities & oxidative stress in women with premature ovarian failure (POF)

BACKGROUND & OBJECTIVES: Premature ovarian failure (POF) is defined as the cessation of ovarian function under the age of 40 yr and is characterized by amenorrhoea, hypoestrogenism and elevated serum gonadotrophin levels. The cause of POF remains undetermined in majority of the cases. This study...

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Autores principales: Kumar, Manoj, Pathak, Dhananjay, Venkatesh, Sundararajan, Kriplani, Alka, Ammini, A.C., Dada, Rima
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3307192/
https://www.ncbi.nlm.nih.gov/pubmed/22382189
http://dx.doi.org/10.4103/0971-5916.93430
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author Kumar, Manoj
Pathak, Dhananjay
Venkatesh, Sundararajan
Kriplani, Alka
Ammini, A.C.
Dada, Rima
author_facet Kumar, Manoj
Pathak, Dhananjay
Venkatesh, Sundararajan
Kriplani, Alka
Ammini, A.C.
Dada, Rima
author_sort Kumar, Manoj
collection PubMed
description BACKGROUND & OBJECTIVES: Premature ovarian failure (POF) is defined as the cessation of ovarian function under the age of 40 yr and is characterized by amenorrhoea, hypoestrogenism and elevated serum gonadotrophin levels. The cause of POF remains undetermined in majority of the cases. This study was aimed to investigate the type and frequency of cytogenetic abnormalities in patients with idiopathic POF and also to study the role of oxidative stress in such cases. METHODS: Seventy five women with idiopathic POF were included in this study. Chromosome analysis was done in peripheral blood lymphocytes by conventional GTG banding to identify numerical or structural abnormalities. Cytogenetically normal cases were investigated for reactive oxygen species (ROS) levels in their blood by luminol-chemiluminescence assay. RESULTS: Eighteen chromosomal anomalies were identified in POF patients (24%). Majority of the cases were found to have X-chromosome abnormalities (28%). Overall median ROS range was found to be significantly higher (P<0.01) in POF patients [50480 (120,132966) RLU/min] compared to controls [340 (120,5094) RLU/min]. Among these, 50 per cent of the POF patients had higher ROS levels, 20 per cent had medium elevation and 30 per cent were found to have normal values comparable to controls. INTERPRETATION & CONCLUSIONS: X-chromosome anomalies were found to be the major contributor of POF. Oxidative stress may be the underlying aetiology in idiopathic premature ovarian failure. Thus the results of this study highlight the role of cytogenetic abnormalities and supraphysiological levels of ROS in causation of idiopathic POF. But the role of oxidative stress needs to be confirmed by other studies on patients from different geographical areas and from different ethnicities.
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spelling pubmed-33071922012-03-21 Chromosomal abnormalities & oxidative stress in women with premature ovarian failure (POF) Kumar, Manoj Pathak, Dhananjay Venkatesh, Sundararajan Kriplani, Alka Ammini, A.C. Dada, Rima Indian J Med Res Original Article BACKGROUND & OBJECTIVES: Premature ovarian failure (POF) is defined as the cessation of ovarian function under the age of 40 yr and is characterized by amenorrhoea, hypoestrogenism and elevated serum gonadotrophin levels. The cause of POF remains undetermined in majority of the cases. This study was aimed to investigate the type and frequency of cytogenetic abnormalities in patients with idiopathic POF and also to study the role of oxidative stress in such cases. METHODS: Seventy five women with idiopathic POF were included in this study. Chromosome analysis was done in peripheral blood lymphocytes by conventional GTG banding to identify numerical or structural abnormalities. Cytogenetically normal cases were investigated for reactive oxygen species (ROS) levels in their blood by luminol-chemiluminescence assay. RESULTS: Eighteen chromosomal anomalies were identified in POF patients (24%). Majority of the cases were found to have X-chromosome abnormalities (28%). Overall median ROS range was found to be significantly higher (P<0.01) in POF patients [50480 (120,132966) RLU/min] compared to controls [340 (120,5094) RLU/min]. Among these, 50 per cent of the POF patients had higher ROS levels, 20 per cent had medium elevation and 30 per cent were found to have normal values comparable to controls. INTERPRETATION & CONCLUSIONS: X-chromosome anomalies were found to be the major contributor of POF. Oxidative stress may be the underlying aetiology in idiopathic premature ovarian failure. Thus the results of this study highlight the role of cytogenetic abnormalities and supraphysiological levels of ROS in causation of idiopathic POF. But the role of oxidative stress needs to be confirmed by other studies on patients from different geographical areas and from different ethnicities. Medknow Publications & Media Pvt Ltd 2012-01 /pmc/articles/PMC3307192/ /pubmed/22382189 http://dx.doi.org/10.4103/0971-5916.93430 Text en Copyright: © The Indian Journal of Medical Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kumar, Manoj
Pathak, Dhananjay
Venkatesh, Sundararajan
Kriplani, Alka
Ammini, A.C.
Dada, Rima
Chromosomal abnormalities & oxidative stress in women with premature ovarian failure (POF)
title Chromosomal abnormalities & oxidative stress in women with premature ovarian failure (POF)
title_full Chromosomal abnormalities & oxidative stress in women with premature ovarian failure (POF)
title_fullStr Chromosomal abnormalities & oxidative stress in women with premature ovarian failure (POF)
title_full_unstemmed Chromosomal abnormalities & oxidative stress in women with premature ovarian failure (POF)
title_short Chromosomal abnormalities & oxidative stress in women with premature ovarian failure (POF)
title_sort chromosomal abnormalities & oxidative stress in women with premature ovarian failure (pof)
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3307192/
https://www.ncbi.nlm.nih.gov/pubmed/22382189
http://dx.doi.org/10.4103/0971-5916.93430
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