Cargando…

Dynamic Mechanism of Proton Transfer in Mannitol 2-Dehydrogenase from Pseudomonas fluorescens: MOBILE GLU(292) CONTROLS PROTON RELAY THROUGH A WATER CHANNEL THAT CONNECTS THE ACTIVE SITE WITH BULK SOLVENT

The active site of mannitol 2-dehydrogenase from Pseudomonas fluorescens (PfM2DH) is connected with bulk solvent through a narrow protein channel that shows structural resemblance to proton channels utilized by redox-driven proton pumps. A key element of the PfM2DH channel is the “mobile” Glu(292),...

Descripción completa

Detalles Bibliográficos
Autores principales:	Klimacek, Mario, Brunsteiner, Michael, Nidetzky, Bernd
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society for Biochemistry and Molecular Biology 2012
Materias:	Enzymology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3307286/ https://www.ncbi.nlm.nih.gov/pubmed/22194597 http://dx.doi.org/10.1074/jbc.M111.289223

Ejemplares similares

Structural and Kinetic Evidence That Catalytic Reaction of Human UDP-glucose 6-Dehydrogenase Involves Covalent Thiohemiacetal and Thioester Enzyme Intermediates
por: Egger, Sigrid, et al.
Publicado: (2012)

Structure and Mechanism of Human UDP-glucose 6-Dehydrogenase
por: Egger, Sigrid, et al.
Publicado: (2011)

Structure and Mechanism of Human UDP-xylose Synthase: EVIDENCE FOR A PROMOTING ROLE OF SUGAR RING DISTORTION IN A THREE-STEP CATALYTIC CONVERSION OF UDP-GLUCURONIC ACID
por: Eixelsberger, Thomas, et al.
Publicado: (2012)

The Relay/Converter Interface Influences Hydrolysis of ATP by Skeletal Muscle Myosin II
por: Bloemink, Marieke J., et al.
Publicado: (2016)

Cellulases Dig Deep: IN SITU OBSERVATION OF THE MESOSCOPIC STRUCTURAL DYNAMICS OF ENZYMATIC CELLULOSE DEGRADATION
por: Bubner, Patricia, et al.
Publicado: (2012)

Investigating the reaction and substrate preference of indole-3-acetaldehyde dehydrogenase from the plant pathogen Pseudomonas syringae PtoDC3000
por: Zhang, Kaleena, et al.
Publicado: (2020)

Dissecting and Reconstructing Synergism: IN SITU VISUALIZATION OF COOPERATIVITY AMONG CELLULASES
por: Ganner, Thomas, et al.
Publicado: (2012)

Formation of Nitric Oxide by Aldehyde Dehydrogenase-2 Is Necessary and Sufficient for Vascular Bioactivation of Nitroglycerin
por: Opelt, Marissa, et al.
Publicado: (2016)

Vascular Bioactivation of Nitroglycerin by Aldehyde Dehydrogenase-2: REACTION INTERMEDIATES REVEALED BY CRYSTALLOGRAPHY AND MASS SPECTROMETRY
por: Lang, Barbara S., et al.
Publicado: (2012)

Cellulose Surface Degradation by a Lytic Polysaccharide Monooxygenase and Its Effect on Cellulase Hydrolytic Efficiency
por: Eibinger, Manuel, et al.
Publicado: (2014)

Structural basis for substrate specificity of methylsuccinyl-CoA dehydrogenase, an unusual member of the acyl-CoA dehydrogenase family
por: Schwander, Thomas, et al.
Publicado: (2018)

A ferredoxin-dependent dihydropyrimidine dehydrogenase in Clostridium chromiireducens
por: Wang, Feifei, et al.
Publicado: (2020)

The Catalytic Aspartate Is Protonated in the Michaelis Complex Formed between Trypsin and an in Vitro Evolved Substrate-like Inhibitor: A REFINED MECHANISM OF SERINE PROTEASE ACTION
por: Wahlgren, Weixiao Yuan, et al.
Publicado: (2011)

Oxidation of Monolignols by Members of the Berberine Bridge Enzyme Family Suggests a Role in Plant Cell Wall Metabolism
por: Daniel, Bastian, et al.
Publicado: (2015)

Origin of the Proton-transfer Step in the Cofactor-free (1H)-3-Hydroxy-4-oxoquinaldine 2,4-Dioxygenase: EFFECT OF THE BASICITY OF AN ACTIVE SITE HIS RESIDUE
por: Hernandez-Ortega, Aitor, et al.
Publicado: (2014)

Heme ligation and redox chemistry in two bacterial thiosulfate dehydrogenase (TsdA) enzymes
por: Jenner, Leon P., et al.
Publicado: (2019)

Diversified amino acid-mediated allosteric regulation of phosphoglycerate dehydrogenase for serine biosynthesis in land plants
por: Okamura, Eiji, et al.
Publicado: (2021)

A pipeline to evaluate inhibitors of the Pseudomonas aeruginosa exotoxin U
por: Foulkes, Daniel M., et al.
Publicado: (2021)

The putative Escherichia coli dehydrogenase YjhC metabolises two dehydrated forms of N-acetylneuraminate produced by some sialidases
por: Kentache, Takfarinas, et al.
Publicado: (2020)

Double agent indole-3-acetic acid: mechanistic analysis of indole-3-acetaldehyde dehydrogenase AldA that synthesizes IAA, an auxin that aids bacterial virulence
por: Shah, Ateek, et al.
Publicado: (2021)

Structure and kinetics of indole-3-glycerol phosphate synthase from Pseudomonas aeruginosa: Decarboxylation is not essential for indole formation
por: Söderholm, Annika, et al.
Publicado: (2020)

PqsBC, a Condensing Enzyme in the Biosynthesis of the Pseudomonas aeruginosa Quinolone Signal: CRYSTAL STRUCTURE, INHIBITION, AND REACTION MECHANISM
por: Drees, Steffen Lorenz, et al.
Publicado: (2016)

Selective Irreversible Inhibition of Neuronal and Inducible Nitric-oxide Synthase in the Combined Presence of Hydrogen Sulfide and Nitric Oxide
por: Heine, Christian L., et al.
Publicado: (2015)

Analysis and prediction of the physiological effects of altered coenzyme specificity in xylose reductase and xylitol dehydrogenase during xylose fermentation by Saccharomyces cerevisiae
por: Krahulec, Stefan, et al.
Publicado: (2012)

Functional Characterization of the Mannitol Promoter of Pseudomonas fluorescens DSM 50106 and Its Application for a Mannitol-Inducible Expression System for Pseudomonas putida KT2440
por: Hoffmann, Jana, et al.
Publicado: (2015)

Catalytic mechanism of human UDP-glucose 6-dehydrogenase: in situ proton NMR studies reveal that the C-5 hydrogen of UDP-glucose is not exchanged with bulk water during the enzymatic reaction
por: Eixelsberger, Thomas, et al.
Publicado: (2012)

Structural Characterization and Ligand/Inhibitor Identification Provide Functional Insights into the Mycobacterium tuberculosis Cytochrome P450 CYP126A1
por: Chenge, Jude T., et al.
Publicado: (2017)

Expanding the Enzyme Repertoire for Sugar Nucleotide Epimerization: the CDP-Tyvelose 2-Epimerase from Thermodesulfatator atlanticus for Glucose/Mannose Interconversion
por: Rapp, Christian, et al.
Publicado: (2021)

Comparative Kinetic and Functional Characterization of the Motor Domains of Human Nonmuscle Myosin-2C Isoforms
por: Heissler, Sarah M., et al.
Publicado: (2011)

Regulation of Response Regulator Autophosphorylation through Interdomain Contacts
por: Barbieri, Christopher M., et al.
Publicado: (2010)

Characterization of the Elongation Complex of Dengue Virus RNA Polymerase: ASSEMBLY, KINETICS OF NUCLEOTIDE INCORPORATION, AND FIDELITY
por: Jin, Zhinan, et al.
Publicado: (2011)

Molecular Characterization of a Novel Geranylgeranyl Pyrophosphate Synthase from Plasmodium Parasites
por: Artz, Jennifer D., et al.
Publicado: (2011)

Quantitative Mass Spectrometry Reveals Dynamics of Factor-inhibiting Hypoxia-inducible Factor-catalyzed Hydroxylation
por: Singleton, Rachelle S., et al.
Publicado: (2011)

Structure and Activity of Paenibacillus polymyxa Xyloglucanase from Glycoside Hydrolase Family 44
por: Ariza, Antonio, et al.
Publicado: (2011)

Characterization of the Deoxynucleotide Triphosphate Triphosphohydrolase (dNTPase) Activity of the EF1143 Protein from Enterococcus faecalis and Crystal Structure of the Activator-Substrate Complex
por: Vorontsov, Ivan I., et al.
Publicado: (2011)

NrdH-Redoxin Protein Mediates High Enzyme Activity in Manganese-reconstituted Ribonucleotide Reductase from Bacillus anthracis
por: Crona, Mikael, et al.
Publicado: (2011)

Purification and Identification of Activating Enzymes of CS-0777, a Selective Sphingosine 1-Phosphate Receptor 1 Modulator, in Erythrocytes
por: Yonesu, Kiyoaki, et al.
Publicado: (2011)

Two Drosophila Myosin Transducer Mutants with Distinct Cardiomyopathies Have Divergent ADP and Actin Affinities
por: Bloemink, Marieke J., et al.
Publicado: (2011)

Comparative Analysis of Histophilus somni Immunoglobulin-binding Protein A (IbpA) with Other Fic Domain-containing Enzymes Reveals Differences in Substrate and Nucleotide Specificities
por: Mattoo, Seema, et al.
Publicado: (2011)

Caspase-1 Promiscuity Is Counterbalanced by Rapid Inactivation of Processed Enzyme
por: Walsh, John G., et al.
Publicado: (2011)

Cannot write session to /tmp/vufind_sessions/sess_ntgqot7b93jna86b505oqllii6