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Cigarette Smoke Affects Keratinocytes SRB1 Expression and Localization via H(2)O(2) Production and HNE Protein Adducts Formation

Scavenger Receptor B1 (SR-B1), also known as HDL receptor, is involved in cellular cholesterol uptake. Stratum corneum (SC), the outermost layer of the skin, is composed of more than 25% cholesterol. Several reports support the view that alteration of SC lipid composition may be the cause of impaire...

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Autores principales: Sticozzi, Claudia, Belmonte, Giuseppe, Pecorelli, Alessandra, Arezzini, Beatrice, Gardi, Concetta, Maioli, Emanuela, Miracco, Clelia, Toscano, Marzia, Forman, Henry Jay, Valacchi, Giuseppe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3307738/
https://www.ncbi.nlm.nih.gov/pubmed/22442701
http://dx.doi.org/10.1371/journal.pone.0033592
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author Sticozzi, Claudia
Belmonte, Giuseppe
Pecorelli, Alessandra
Arezzini, Beatrice
Gardi, Concetta
Maioli, Emanuela
Miracco, Clelia
Toscano, Marzia
Forman, Henry Jay
Valacchi, Giuseppe
author_facet Sticozzi, Claudia
Belmonte, Giuseppe
Pecorelli, Alessandra
Arezzini, Beatrice
Gardi, Concetta
Maioli, Emanuela
Miracco, Clelia
Toscano, Marzia
Forman, Henry Jay
Valacchi, Giuseppe
author_sort Sticozzi, Claudia
collection PubMed
description Scavenger Receptor B1 (SR-B1), also known as HDL receptor, is involved in cellular cholesterol uptake. Stratum corneum (SC), the outermost layer of the skin, is composed of more than 25% cholesterol. Several reports support the view that alteration of SC lipid composition may be the cause of impaired barrier function which gives rise to several skin diseases. For this reason the regulation of the genes involved in cholesterol uptake is of extreme significance for skin health. Being the first shield against external insults, the skin is exposed to several noxious substances and among these is cigarette smoke (CS), which has been recently associated with various skin pathologies. In this study we first have shown the presence of SR-B1 in murine and human skin tissue and then by using immunoblotting, immunoprecipitation, RT-PCR, and confocal microscopy we have demonstrated the translocation and the subsequent lost of SR-B1 in human keratinocytes (cell culture model) after CS exposure is driven by hydrogen peroxide (H(2)O(2)) that derives not only from the CS gas phase but mainly from the activation of cellular NADPH oxidase (NOX). This effect was reversed when the cells were pretreated with NOX inhibitors or catalase. Furthermore, CS caused the formation of SR-B1-aldheydes adducts (acrolein and 4-hydroxy-2-nonenal) and the increase of its ubiquitination, which could be one of the causes of SR-B1 loss. In conclusion, exposure to CS, through the production of H(2)O(2), induced post-translational modifications of SR-B1 with the consequence lost of the receptor and this may contribute to the skin physiology alteration as a consequence of the variation of cholesterol uptake.
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spelling pubmed-33077382012-03-22 Cigarette Smoke Affects Keratinocytes SRB1 Expression and Localization via H(2)O(2) Production and HNE Protein Adducts Formation Sticozzi, Claudia Belmonte, Giuseppe Pecorelli, Alessandra Arezzini, Beatrice Gardi, Concetta Maioli, Emanuela Miracco, Clelia Toscano, Marzia Forman, Henry Jay Valacchi, Giuseppe PLoS One Research Article Scavenger Receptor B1 (SR-B1), also known as HDL receptor, is involved in cellular cholesterol uptake. Stratum corneum (SC), the outermost layer of the skin, is composed of more than 25% cholesterol. Several reports support the view that alteration of SC lipid composition may be the cause of impaired barrier function which gives rise to several skin diseases. For this reason the regulation of the genes involved in cholesterol uptake is of extreme significance for skin health. Being the first shield against external insults, the skin is exposed to several noxious substances and among these is cigarette smoke (CS), which has been recently associated with various skin pathologies. In this study we first have shown the presence of SR-B1 in murine and human skin tissue and then by using immunoblotting, immunoprecipitation, RT-PCR, and confocal microscopy we have demonstrated the translocation and the subsequent lost of SR-B1 in human keratinocytes (cell culture model) after CS exposure is driven by hydrogen peroxide (H(2)O(2)) that derives not only from the CS gas phase but mainly from the activation of cellular NADPH oxidase (NOX). This effect was reversed when the cells were pretreated with NOX inhibitors or catalase. Furthermore, CS caused the formation of SR-B1-aldheydes adducts (acrolein and 4-hydroxy-2-nonenal) and the increase of its ubiquitination, which could be one of the causes of SR-B1 loss. In conclusion, exposure to CS, through the production of H(2)O(2), induced post-translational modifications of SR-B1 with the consequence lost of the receptor and this may contribute to the skin physiology alteration as a consequence of the variation of cholesterol uptake. Public Library of Science 2012-03-19 /pmc/articles/PMC3307738/ /pubmed/22442701 http://dx.doi.org/10.1371/journal.pone.0033592 Text en Sticozzi et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Sticozzi, Claudia
Belmonte, Giuseppe
Pecorelli, Alessandra
Arezzini, Beatrice
Gardi, Concetta
Maioli, Emanuela
Miracco, Clelia
Toscano, Marzia
Forman, Henry Jay
Valacchi, Giuseppe
Cigarette Smoke Affects Keratinocytes SRB1 Expression and Localization via H(2)O(2) Production and HNE Protein Adducts Formation
title Cigarette Smoke Affects Keratinocytes SRB1 Expression and Localization via H(2)O(2) Production and HNE Protein Adducts Formation
title_full Cigarette Smoke Affects Keratinocytes SRB1 Expression and Localization via H(2)O(2) Production and HNE Protein Adducts Formation
title_fullStr Cigarette Smoke Affects Keratinocytes SRB1 Expression and Localization via H(2)O(2) Production and HNE Protein Adducts Formation
title_full_unstemmed Cigarette Smoke Affects Keratinocytes SRB1 Expression and Localization via H(2)O(2) Production and HNE Protein Adducts Formation
title_short Cigarette Smoke Affects Keratinocytes SRB1 Expression and Localization via H(2)O(2) Production and HNE Protein Adducts Formation
title_sort cigarette smoke affects keratinocytes srb1 expression and localization via h(2)o(2) production and hne protein adducts formation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3307738/
https://www.ncbi.nlm.nih.gov/pubmed/22442701
http://dx.doi.org/10.1371/journal.pone.0033592
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