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Identifying New Therapeutic Targets via Modulation of Protein Corona Formation by Engineered Nanoparticles
BACKGROUND: We introduce a promising methodology to identify new therapeutic targets in cancer. Proteins bind to nanoparticles to form a protein corona. We modulate this corona by using surface-engineered nanoparticles, and identify protein composition to provide insight into disease development. ME...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3307759/ https://www.ncbi.nlm.nih.gov/pubmed/22442705 http://dx.doi.org/10.1371/journal.pone.0033650 |
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author | Arvizo, Rochelle R. Giri, Karuna Moyano, Daniel Miranda, Oscar R. Madden, Benjamin McCormick, Daniel J. Bhattacharya, Resham Rotello, Vincent M. Kocher, Jean-Pierre Mukherjee, Priyabrata |
author_facet | Arvizo, Rochelle R. Giri, Karuna Moyano, Daniel Miranda, Oscar R. Madden, Benjamin McCormick, Daniel J. Bhattacharya, Resham Rotello, Vincent M. Kocher, Jean-Pierre Mukherjee, Priyabrata |
author_sort | Arvizo, Rochelle R. |
collection | PubMed |
description | BACKGROUND: We introduce a promising methodology to identify new therapeutic targets in cancer. Proteins bind to nanoparticles to form a protein corona. We modulate this corona by using surface-engineered nanoparticles, and identify protein composition to provide insight into disease development. METHODS/PRINCIPAL FINDINGS: Using a family of structurally homologous nanoparticles we have investigated the changes in the protein corona around surface-functionalized gold nanoparticles (AuNPs) from normal and malignant ovarian cell lysates. Proteomics analysis using mass spectrometry identified hepatoma-derived growth factor (HDGF) that is found exclusively on positively charged AuNPs ((+)AuNPs) after incubation with the lysates. We confirmed expression of HDGF in various ovarian cancer cells and validated binding selectivity to (+)AuNPs by Western blot analysis. Silencing of HDGF by siRNA resulted s inhibition in proliferation of ovarian cancer cells. CONCLUSION: We investigated the modulation of protein corona around surface-functionalized gold nanoparticles as a promising approach to identify new therapeutic targets. The potential of our method for identifying therapeutic targets was demonstrated through silencing of HDGF by siRNA, which inhibited proliferation of ovarian cancer cells. This integrated proteomics, bioinformatics, and nanotechnology strategy demonstrates that protein corona identification can be used to discover novel therapeutic targets in cancer. |
format | Online Article Text |
id | pubmed-3307759 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-33077592012-03-22 Identifying New Therapeutic Targets via Modulation of Protein Corona Formation by Engineered Nanoparticles Arvizo, Rochelle R. Giri, Karuna Moyano, Daniel Miranda, Oscar R. Madden, Benjamin McCormick, Daniel J. Bhattacharya, Resham Rotello, Vincent M. Kocher, Jean-Pierre Mukherjee, Priyabrata PLoS One Research Article BACKGROUND: We introduce a promising methodology to identify new therapeutic targets in cancer. Proteins bind to nanoparticles to form a protein corona. We modulate this corona by using surface-engineered nanoparticles, and identify protein composition to provide insight into disease development. METHODS/PRINCIPAL FINDINGS: Using a family of structurally homologous nanoparticles we have investigated the changes in the protein corona around surface-functionalized gold nanoparticles (AuNPs) from normal and malignant ovarian cell lysates. Proteomics analysis using mass spectrometry identified hepatoma-derived growth factor (HDGF) that is found exclusively on positively charged AuNPs ((+)AuNPs) after incubation with the lysates. We confirmed expression of HDGF in various ovarian cancer cells and validated binding selectivity to (+)AuNPs by Western blot analysis. Silencing of HDGF by siRNA resulted s inhibition in proliferation of ovarian cancer cells. CONCLUSION: We investigated the modulation of protein corona around surface-functionalized gold nanoparticles as a promising approach to identify new therapeutic targets. The potential of our method for identifying therapeutic targets was demonstrated through silencing of HDGF by siRNA, which inhibited proliferation of ovarian cancer cells. This integrated proteomics, bioinformatics, and nanotechnology strategy demonstrates that protein corona identification can be used to discover novel therapeutic targets in cancer. Public Library of Science 2012-03-19 /pmc/articles/PMC3307759/ /pubmed/22442705 http://dx.doi.org/10.1371/journal.pone.0033650 Text en Arvizo et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Arvizo, Rochelle R. Giri, Karuna Moyano, Daniel Miranda, Oscar R. Madden, Benjamin McCormick, Daniel J. Bhattacharya, Resham Rotello, Vincent M. Kocher, Jean-Pierre Mukherjee, Priyabrata Identifying New Therapeutic Targets via Modulation of Protein Corona Formation by Engineered Nanoparticles |
title | Identifying New Therapeutic Targets via Modulation of Protein Corona Formation by Engineered Nanoparticles |
title_full | Identifying New Therapeutic Targets via Modulation of Protein Corona Formation by Engineered Nanoparticles |
title_fullStr | Identifying New Therapeutic Targets via Modulation of Protein Corona Formation by Engineered Nanoparticles |
title_full_unstemmed | Identifying New Therapeutic Targets via Modulation of Protein Corona Formation by Engineered Nanoparticles |
title_short | Identifying New Therapeutic Targets via Modulation of Protein Corona Formation by Engineered Nanoparticles |
title_sort | identifying new therapeutic targets via modulation of protein corona formation by engineered nanoparticles |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3307759/ https://www.ncbi.nlm.nih.gov/pubmed/22442705 http://dx.doi.org/10.1371/journal.pone.0033650 |
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