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Increase in IL-21 producing T-cells in patients with systemic lupus erythematosus

INTRODUCTION: Systemic lupus erythematosus (SLE) is an autoimmune disease accompanied by a disturbed T-cell balance skewed towards effector T-cells, in particular Th17-cells. The novel cytokine interleukin-21 (IL-21) is suggested to be crucial for triggering T-cell responses towards IL-17 producing...

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Autores principales: Dolff, Sebastian, Abdulahad, Wayel H, Westra, Johanna, Doornbos-van der Meer, Berber, Limburg, Pieter C, Kallenberg, Cees GM, Bijl, Marc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3308088/
https://www.ncbi.nlm.nih.gov/pubmed/21959034
http://dx.doi.org/10.1186/ar3474
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author Dolff, Sebastian
Abdulahad, Wayel H
Westra, Johanna
Doornbos-van der Meer, Berber
Limburg, Pieter C
Kallenberg, Cees GM
Bijl, Marc
author_facet Dolff, Sebastian
Abdulahad, Wayel H
Westra, Johanna
Doornbos-van der Meer, Berber
Limburg, Pieter C
Kallenberg, Cees GM
Bijl, Marc
author_sort Dolff, Sebastian
collection PubMed
description INTRODUCTION: Systemic lupus erythematosus (SLE) is an autoimmune disease accompanied by a disturbed T-cell balance skewed towards effector T-cells, in particular Th17-cells. The novel cytokine interleukin-21 (IL-21) is suggested to be crucial for triggering T-cell responses towards IL-17 producing cells. Thus, we aimed to investigate the ability of T-cells to produce IL-21 and IL-17 in SLE patients. METHODS: Peripheral blood of 34 SLE patients and 18 healthy controls (HC) was stimulated with phorbol myristate acetate (PMA) and calcium ionophore (Ca-Io). Percentages of IL-21- and IL-17A expressing T-cells were analysed by flow cytometry. The expression levels of the transcription factors B-cell lymphoma-6 (BCL-6) and factors retinoid-related orphan receptor (ROR-γt) were assessed in T-cells by real-time RT-PCR and flow cytometry. Additionally, IL-21 receptor (IL-21R) expression on B- and T-cells of patients and HC was analyzed. RESULTS: Significantly increased percentages of IL-21 expressing CD4(+ )T-cells and CD8(+ )T-cells were found in SLE patients as compared to HC. The percentages of IL-21(+ )CD4(+ )T-cells and CD8(+ )T-cells correlated significantly with the percentages of IL-17A(+ )CD4(+ )T-cells and CD8(+ )T-cells, respectively. The relative expression of BCL-6 and ROR-γt did not differ between SLE patients and HC. IL-21R expression occurred mainly on B-cells and was not different comparing SLE patients and HC. CONCLUSIONS: This study demonstrates an increased proportion of IL-21(+ )T-cells in SLE patients correlating with the proportion of IL-17(+ )T-cells. This suggests a pivotal role of IL-21 in the pathogenesis of SLE.
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spelling pubmed-33080882012-03-20 Increase in IL-21 producing T-cells in patients with systemic lupus erythematosus Dolff, Sebastian Abdulahad, Wayel H Westra, Johanna Doornbos-van der Meer, Berber Limburg, Pieter C Kallenberg, Cees GM Bijl, Marc Arthritis Res Ther Research Article INTRODUCTION: Systemic lupus erythematosus (SLE) is an autoimmune disease accompanied by a disturbed T-cell balance skewed towards effector T-cells, in particular Th17-cells. The novel cytokine interleukin-21 (IL-21) is suggested to be crucial for triggering T-cell responses towards IL-17 producing cells. Thus, we aimed to investigate the ability of T-cells to produce IL-21 and IL-17 in SLE patients. METHODS: Peripheral blood of 34 SLE patients and 18 healthy controls (HC) was stimulated with phorbol myristate acetate (PMA) and calcium ionophore (Ca-Io). Percentages of IL-21- and IL-17A expressing T-cells were analysed by flow cytometry. The expression levels of the transcription factors B-cell lymphoma-6 (BCL-6) and factors retinoid-related orphan receptor (ROR-γt) were assessed in T-cells by real-time RT-PCR and flow cytometry. Additionally, IL-21 receptor (IL-21R) expression on B- and T-cells of patients and HC was analyzed. RESULTS: Significantly increased percentages of IL-21 expressing CD4(+ )T-cells and CD8(+ )T-cells were found in SLE patients as compared to HC. The percentages of IL-21(+ )CD4(+ )T-cells and CD8(+ )T-cells correlated significantly with the percentages of IL-17A(+ )CD4(+ )T-cells and CD8(+ )T-cells, respectively. The relative expression of BCL-6 and ROR-γt did not differ between SLE patients and HC. IL-21R expression occurred mainly on B-cells and was not different comparing SLE patients and HC. CONCLUSIONS: This study demonstrates an increased proportion of IL-21(+ )T-cells in SLE patients correlating with the proportion of IL-17(+ )T-cells. This suggests a pivotal role of IL-21 in the pathogenesis of SLE. BioMed Central 2011 2011-09-29 /pmc/articles/PMC3308088/ /pubmed/21959034 http://dx.doi.org/10.1186/ar3474 Text en Copyright ©2011 Dolff et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Dolff, Sebastian
Abdulahad, Wayel H
Westra, Johanna
Doornbos-van der Meer, Berber
Limburg, Pieter C
Kallenberg, Cees GM
Bijl, Marc
Increase in IL-21 producing T-cells in patients with systemic lupus erythematosus
title Increase in IL-21 producing T-cells in patients with systemic lupus erythematosus
title_full Increase in IL-21 producing T-cells in patients with systemic lupus erythematosus
title_fullStr Increase in IL-21 producing T-cells in patients with systemic lupus erythematosus
title_full_unstemmed Increase in IL-21 producing T-cells in patients with systemic lupus erythematosus
title_short Increase in IL-21 producing T-cells in patients with systemic lupus erythematosus
title_sort increase in il-21 producing t-cells in patients with systemic lupus erythematosus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3308088/
https://www.ncbi.nlm.nih.gov/pubmed/21959034
http://dx.doi.org/10.1186/ar3474
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