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Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
Laser axotomy followed by time-lapse microscopy is a sensitive assay for axon regeneration phenotypes in C. elegans(1). The main difficulty of this assay is the perceived cost ($25-100K) and technical expertise required for implementing a laser ablation system(2,3). However, solid-state pulse lasers...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MyJove Corporation
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3308599/ https://www.ncbi.nlm.nih.gov/pubmed/22126922 http://dx.doi.org/10.3791/3331 |