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Changes in the Muscarinic Receptors on the Colonic Smooth Muscles of Rats with Spinal Cord Injury

OBJECTIVE: To investigate changes in (1) the colonic response to acetylcholine (Ach), (2) the muscarinic (M) receptors in the colon, and (3) the levels of colonic contraction-related proteins after a spinal cord injury (SCI). METHOD: We divided 16 Sprague-Dawley rats into 2 groups: the control group...

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Autores principales: Joo, Min Cheol, Kim, Yong Sung, Choi, Eul Sik, Oh, Jung Taek, Park, Hyun Joon, Lee, Moon Young
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Academy of Rehabilitation Medicine 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3309258/
https://www.ncbi.nlm.nih.gov/pubmed/22506180
http://dx.doi.org/10.5535/arm.2011.35.5.589
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author Joo, Min Cheol
Kim, Yong Sung
Choi, Eul Sik
Oh, Jung Taek
Park, Hyun Joon
Lee, Moon Young
author_facet Joo, Min Cheol
Kim, Yong Sung
Choi, Eul Sik
Oh, Jung Taek
Park, Hyun Joon
Lee, Moon Young
author_sort Joo, Min Cheol
collection PubMed
description OBJECTIVE: To investigate changes in (1) the colonic response to acetylcholine (Ach), (2) the muscarinic (M) receptors in the colon, and (3) the levels of colonic contraction-related proteins after a spinal cord injury (SCI). METHOD: We divided 16 Sprague-Dawley rats into 2 groups: the control group and the SCI group. A spinal cord transection was performed surgically at the T10 vertebral level. After 1 week, the entire colon was divided into 2 segments, the proximal and distal colon. Each segment was mounted in a longitudinal or circular muscle direction in a 10-ml organ bath. We determined the intergroup differences as percentage changes in contractility after Ach treatment alone, Ach treatment with M2 receptor antagonist (AQ-RA741) pretreatment, and Ach treatment with M3 receptor antagonist (4-DAMP) pretreatment. Western blot analyses were performed to determine the expression level of RhoA, and heat shock protein 27 (HSP27). RESULTS: Compared to the control rats, the SCI rats showed an increased response to Ach along both the directions in the proximal colon (p<0.05). Compared to the control group, in the SCI group, the Ach response was significantly different in the proximal segment under AQ-RA741 pretreatment (p<0.05) and in the distal segment under 4-DAMP pretreatment (p<0.05). Findings of the western blot analyses showed a significant decrease in the level of protein gene product 9.5 in the proximal and distal colon and a significant increase in the level of RhoA and HSP27 in the proximal colon of the SCI rats. CONCLUSION: Our results suggest that changes in colonic contractility after SCI are partly attributable to changes in the M receptor subtypes.
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spelling pubmed-33092582012-04-04 Changes in the Muscarinic Receptors on the Colonic Smooth Muscles of Rats with Spinal Cord Injury Joo, Min Cheol Kim, Yong Sung Choi, Eul Sik Oh, Jung Taek Park, Hyun Joon Lee, Moon Young Ann Rehabil Med Original Article OBJECTIVE: To investigate changes in (1) the colonic response to acetylcholine (Ach), (2) the muscarinic (M) receptors in the colon, and (3) the levels of colonic contraction-related proteins after a spinal cord injury (SCI). METHOD: We divided 16 Sprague-Dawley rats into 2 groups: the control group and the SCI group. A spinal cord transection was performed surgically at the T10 vertebral level. After 1 week, the entire colon was divided into 2 segments, the proximal and distal colon. Each segment was mounted in a longitudinal or circular muscle direction in a 10-ml organ bath. We determined the intergroup differences as percentage changes in contractility after Ach treatment alone, Ach treatment with M2 receptor antagonist (AQ-RA741) pretreatment, and Ach treatment with M3 receptor antagonist (4-DAMP) pretreatment. Western blot analyses were performed to determine the expression level of RhoA, and heat shock protein 27 (HSP27). RESULTS: Compared to the control rats, the SCI rats showed an increased response to Ach along both the directions in the proximal colon (p<0.05). Compared to the control group, in the SCI group, the Ach response was significantly different in the proximal segment under AQ-RA741 pretreatment (p<0.05) and in the distal segment under 4-DAMP pretreatment (p<0.05). Findings of the western blot analyses showed a significant decrease in the level of protein gene product 9.5 in the proximal and distal colon and a significant increase in the level of RhoA and HSP27 in the proximal colon of the SCI rats. CONCLUSION: Our results suggest that changes in colonic contractility after SCI are partly attributable to changes in the M receptor subtypes. Korean Academy of Rehabilitation Medicine 2011-10 2011-10-31 /pmc/articles/PMC3309258/ /pubmed/22506180 http://dx.doi.org/10.5535/arm.2011.35.5.589 Text en Copyright © 2011 by Korean Academy of Rehabilitation Medicine http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Joo, Min Cheol
Kim, Yong Sung
Choi, Eul Sik
Oh, Jung Taek
Park, Hyun Joon
Lee, Moon Young
Changes in the Muscarinic Receptors on the Colonic Smooth Muscles of Rats with Spinal Cord Injury
title Changes in the Muscarinic Receptors on the Colonic Smooth Muscles of Rats with Spinal Cord Injury
title_full Changes in the Muscarinic Receptors on the Colonic Smooth Muscles of Rats with Spinal Cord Injury
title_fullStr Changes in the Muscarinic Receptors on the Colonic Smooth Muscles of Rats with Spinal Cord Injury
title_full_unstemmed Changes in the Muscarinic Receptors on the Colonic Smooth Muscles of Rats with Spinal Cord Injury
title_short Changes in the Muscarinic Receptors on the Colonic Smooth Muscles of Rats with Spinal Cord Injury
title_sort changes in the muscarinic receptors on the colonic smooth muscles of rats with spinal cord injury
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3309258/
https://www.ncbi.nlm.nih.gov/pubmed/22506180
http://dx.doi.org/10.5535/arm.2011.35.5.589
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