Hepatitis E Virus in Rats, Los Angeles, California, USA

The role of rats in human hepatitis E virus (HEV) infections remains controversial. A genetically distinct HEV was recently isolated from rats in Germany, and its genome was sequenced. We have isolated a genetically similar HEV from urban rats in Los Angeles, California, USA, and characterized its a...

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Autores principales: Purcell, Robert H., Engle, Ronald E., Rood, Michael P., Kabrane-Lazizi, Yamina, Nguyen, Hanh T., Govindarajan, Sugantha, St. Claire, Marisa, Emerson, Suzanne U.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2011
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3311208/
https://www.ncbi.nlm.nih.gov/pubmed/22172320
http://dx.doi.org/10.3201/eid1712.110482
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author Purcell, Robert H.
Engle, Ronald E.
Rood, Michael P.
Kabrane-Lazizi, Yamina
Nguyen, Hanh T.
Govindarajan, Sugantha
St. Claire, Marisa
Emerson, Suzanne U.
author_facet Purcell, Robert H.
Engle, Ronald E.
Rood, Michael P.
Kabrane-Lazizi, Yamina
Nguyen, Hanh T.
Govindarajan, Sugantha
St. Claire, Marisa
Emerson, Suzanne U.
author_sort Purcell, Robert H.
collection PubMed
description The role of rats in human hepatitis E virus (HEV) infections remains controversial. A genetically distinct HEV was recently isolated from rats in Germany, and its genome was sequenced. We have isolated a genetically similar HEV from urban rats in Los Angeles, California, USA, and characterized its ability to infect laboratory rats and nonhuman primates. Two strains of HEV were isolated from serum samples of 134 wild rats that had a seroprevalence of antibodies against HEV of ≈80%. Virus was transmissible to seronegative Sprague-Dawley rats, but transmission was spotty and magnitude and duration of infection were not robust. Viremia was higher in nude rats. Serologic analysis and reverse transcription PCR were comparably sensitive in detecting infection. The sequence of the Los Angeles virus was virtually identical to that of isolates from Germany. Rat HEV was not transmissible to rhesus monkeys, suggesting that it is not a source of human infection.
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spelling pubmed-33112082012-04-06 Hepatitis E Virus in Rats, Los Angeles, California, USA Purcell, Robert H. Engle, Ronald E. Rood, Michael P. Kabrane-Lazizi, Yamina Nguyen, Hanh T. Govindarajan, Sugantha St. Claire, Marisa Emerson, Suzanne U. Emerg Infect Dis Research The role of rats in human hepatitis E virus (HEV) infections remains controversial. A genetically distinct HEV was recently isolated from rats in Germany, and its genome was sequenced. We have isolated a genetically similar HEV from urban rats in Los Angeles, California, USA, and characterized its ability to infect laboratory rats and nonhuman primates. Two strains of HEV were isolated from serum samples of 134 wild rats that had a seroprevalence of antibodies against HEV of ≈80%. Virus was transmissible to seronegative Sprague-Dawley rats, but transmission was spotty and magnitude and duration of infection were not robust. Viremia was higher in nude rats. Serologic analysis and reverse transcription PCR were comparably sensitive in detecting infection. The sequence of the Los Angeles virus was virtually identical to that of isolates from Germany. Rat HEV was not transmissible to rhesus monkeys, suggesting that it is not a source of human infection. Centers for Disease Control and Prevention 2011-12 /pmc/articles/PMC3311208/ /pubmed/22172320 http://dx.doi.org/10.3201/eid1712.110482 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Research
Purcell, Robert H.
Engle, Ronald E.
Rood, Michael P.
Kabrane-Lazizi, Yamina
Nguyen, Hanh T.
Govindarajan, Sugantha
St. Claire, Marisa
Emerson, Suzanne U.
Hepatitis E Virus in Rats, Los Angeles, California, USA
title Hepatitis E Virus in Rats, Los Angeles, California, USA
title_full Hepatitis E Virus in Rats, Los Angeles, California, USA
title_fullStr Hepatitis E Virus in Rats, Los Angeles, California, USA
title_full_unstemmed Hepatitis E Virus in Rats, Los Angeles, California, USA
title_short Hepatitis E Virus in Rats, Los Angeles, California, USA
title_sort hepatitis e virus in rats, los angeles, california, usa
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3311208/
https://www.ncbi.nlm.nih.gov/pubmed/22172320
http://dx.doi.org/10.3201/eid1712.110482
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