Novel Multiplexed HIV/Simian Immunodeficiency Virus Antibody Detection Assay

Like most emerging infectious disease viruses, HIV is also of zoonotic origin. To assess the risk for cross-species transmission of simian immunodeficiency viruses (SIVs) from nonhuman primates to humans in the Democratic Republic of Congo, we collected 330 samples derived from nonhuman primate bush...

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Detalles Bibliográficos
Autores principales: Ahuka-Mundeke, Steve, Ayouba, Ahidjo, Mbala-Kingebeni, Placide, Liegeois, Florian, Esteban, Amandine, Lunguya-Metila, Octavie, Demba, Didace, Bilulu, Guy, Mbenzo-Abokome, Valentin, Inogwabini, Bila-Isia, Muyembe-Tamfum, Jean-Jacques, Delaporte, Eric, Peeters, Martine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2011
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3311211/
https://www.ncbi.nlm.nih.gov/pubmed/22172157
http://dx.doi.org/10.3201/eid1712.110783
Descripción
Sumario:Like most emerging infectious disease viruses, HIV is also of zoonotic origin. To assess the risk for cross-species transmission of simian immunodeficiency viruses (SIVs) from nonhuman primates to humans in the Democratic Republic of Congo, we collected 330 samples derived from nonhuman primate bushmeat at 3 remote forest sites. SIV prevalences were estimated by using a novel high-throughput assay that included 34 HIV and SIV antigens in a single well. Overall, 19% of nonhuman primate bushmeat was infected with SIVs, and new SIV lineages were identified. Highest SIV prevalences were seen in red-tailed guenons (25%) and Tshuapa red colobus monkeys (24%), representing the most common hunted primate species, thus increasing the likelihood for cross-species transmission. Additional studies are needed to determine whether other SIVs crossed the species barrier. With the newly developed assay, large-scale screening against many antigens is now easier and faster.

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