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DNA Methylation of GSTP1 in Human Prostate Tissues: Pyrosequencing Analysis

PURPOSE: DNA methylation is an important epigenetic mechanism of gene regulation and plays essential roles in tumor initiation and progression. Differences in methylation patterns between neoplastic and normal cells can be used to detect the presence of cancer. The aim of the present study was to ev...

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Autores principales: Yoon, Hyung-Yoon, Kim, Young-Won, Kang, Ho-Won, Kim, Won Tae, Yun, Seok-Joong, Lee, Sang-Cheol, Kim, Wun-Jae, Kim, Yong-June
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Urological Association 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3312070/
https://www.ncbi.nlm.nih.gov/pubmed/22468217
http://dx.doi.org/10.4111/kju.2012.53.3.200
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author Yoon, Hyung-Yoon
Kim, Young-Won
Kang, Ho-Won
Kim, Won Tae
Yun, Seok-Joong
Lee, Sang-Cheol
Kim, Wun-Jae
Kim, Yong-June
author_facet Yoon, Hyung-Yoon
Kim, Young-Won
Kang, Ho-Won
Kim, Won Tae
Yun, Seok-Joong
Lee, Sang-Cheol
Kim, Wun-Jae
Kim, Yong-June
author_sort Yoon, Hyung-Yoon
collection PubMed
description PURPOSE: DNA methylation is an important epigenetic mechanism of gene regulation and plays essential roles in tumor initiation and progression. Differences in methylation patterns between neoplastic and normal cells can be used to detect the presence of cancer. The aim of the present study was to evaluate the usefulness of glutathione-S-transferase-Pi (GSTP1) hypermethylation in discriminating between normal and prostate cancer (PCa) cells and in predicting tumor characteristics by use of quantitative pyrosequencing analysis. MATERIALS AND METHODS: A total of 100 human prostate tissues obtained from our institute were used in this study: 45 for benign prostatic hyperplasia (BPH) and 55 for PCa. The methylation level of GSTP1 was examined by a quantitative pyrosequencing analysis. The associations between GSTP1 methylation level and clinico-pathological parameter were also compared. RESULTS: The level of GSTP1 methylation was significantly higher in PCa samples than in BPH samples (56.7±32.7% vs. 1.6±2.2%, p<0.001). The sensitivity and specificity of GSTP1 methylation status in discriminating between PCa and BPH reached 85.5% and 100%, respectively. Even after stratification by stage, Gleason score, and prostate-specific antigen (PSA) level, similar results were obtained. A positive correlation between GSTP1 methylation level and serum PSA level was observed (r=0.303, p=0.002). There were no associations between GSTP1 methylation level and age, Gleason score, and staging. CONCLUSIONS: Our study demonstrates that GSTP1 methylation is associated with the presence of PCa and PSA levels. This methylation marker is a potentially useful indicator for the detection and monitoring of PCa.
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spelling pubmed-33120702012-03-30 DNA Methylation of GSTP1 in Human Prostate Tissues: Pyrosequencing Analysis Yoon, Hyung-Yoon Kim, Young-Won Kang, Ho-Won Kim, Won Tae Yun, Seok-Joong Lee, Sang-Cheol Kim, Wun-Jae Kim, Yong-June Korean J Urol Original Article PURPOSE: DNA methylation is an important epigenetic mechanism of gene regulation and plays essential roles in tumor initiation and progression. Differences in methylation patterns between neoplastic and normal cells can be used to detect the presence of cancer. The aim of the present study was to evaluate the usefulness of glutathione-S-transferase-Pi (GSTP1) hypermethylation in discriminating between normal and prostate cancer (PCa) cells and in predicting tumor characteristics by use of quantitative pyrosequencing analysis. MATERIALS AND METHODS: A total of 100 human prostate tissues obtained from our institute were used in this study: 45 for benign prostatic hyperplasia (BPH) and 55 for PCa. The methylation level of GSTP1 was examined by a quantitative pyrosequencing analysis. The associations between GSTP1 methylation level and clinico-pathological parameter were also compared. RESULTS: The level of GSTP1 methylation was significantly higher in PCa samples than in BPH samples (56.7±32.7% vs. 1.6±2.2%, p<0.001). The sensitivity and specificity of GSTP1 methylation status in discriminating between PCa and BPH reached 85.5% and 100%, respectively. Even after stratification by stage, Gleason score, and prostate-specific antigen (PSA) level, similar results were obtained. A positive correlation between GSTP1 methylation level and serum PSA level was observed (r=0.303, p=0.002). There were no associations between GSTP1 methylation level and age, Gleason score, and staging. CONCLUSIONS: Our study demonstrates that GSTP1 methylation is associated with the presence of PCa and PSA levels. This methylation marker is a potentially useful indicator for the detection and monitoring of PCa. The Korean Urological Association 2012-03 2012-03-19 /pmc/articles/PMC3312070/ /pubmed/22468217 http://dx.doi.org/10.4111/kju.2012.53.3.200 Text en © The Korean Urological Association, 2012 http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Yoon, Hyung-Yoon
Kim, Young-Won
Kang, Ho-Won
Kim, Won Tae
Yun, Seok-Joong
Lee, Sang-Cheol
Kim, Wun-Jae
Kim, Yong-June
DNA Methylation of GSTP1 in Human Prostate Tissues: Pyrosequencing Analysis
title DNA Methylation of GSTP1 in Human Prostate Tissues: Pyrosequencing Analysis
title_full DNA Methylation of GSTP1 in Human Prostate Tissues: Pyrosequencing Analysis
title_fullStr DNA Methylation of GSTP1 in Human Prostate Tissues: Pyrosequencing Analysis
title_full_unstemmed DNA Methylation of GSTP1 in Human Prostate Tissues: Pyrosequencing Analysis
title_short DNA Methylation of GSTP1 in Human Prostate Tissues: Pyrosequencing Analysis
title_sort dna methylation of gstp1 in human prostate tissues: pyrosequencing analysis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3312070/
https://www.ncbi.nlm.nih.gov/pubmed/22468217
http://dx.doi.org/10.4111/kju.2012.53.3.200
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