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Evaluation of Osteoconductive and Osteogenic Potential of a Dentin-Based Bone Substitute Using a Calvarial Defect Model

The aim of this study was to assess the osteoconductive and osteogenic properties of processed bovine dentin using a robust rabbit calvarial defect model. In total, 16 New Zealand White rabbits were operated to create three circular defects in the calvaria. One defect was left unfilled, one filled w...

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Autores principales: Hussain, Ibrahim, Moharamzadeh, Keyvan, Brook, Ian M., José de Oliveira Neto, Patrício, Salata, Luiz A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3312261/
https://www.ncbi.nlm.nih.gov/pubmed/22505899
http://dx.doi.org/10.1155/2012/396316
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author Hussain, Ibrahim
Moharamzadeh, Keyvan
Brook, Ian M.
José de Oliveira Neto, Patrício
Salata, Luiz A.
author_facet Hussain, Ibrahim
Moharamzadeh, Keyvan
Brook, Ian M.
José de Oliveira Neto, Patrício
Salata, Luiz A.
author_sort Hussain, Ibrahim
collection PubMed
description The aim of this study was to assess the osteoconductive and osteogenic properties of processed bovine dentin using a robust rabbit calvarial defect model. In total, 16 New Zealand White rabbits were operated to create three circular defects in the calvaria. One defect was left unfilled, one filled with collected autogenous bone, and the third defect was filled with the dentin-based bone substitute. Following surgery and after a healing period of either 1 or 6 weeks, a CT scan was obtained. Following sacrificing, the tissues were processed for histological examination. The CT data showed the density in the area grafted with the dentin-based material was higher than the surrounding bone and the areas grafted with autologous bone after 1 week and 6 weeks of healing. The area left unfilled remained an empty defect after 1 week and 6 weeks. Histological examination of the defects filled with the dentin product after 6 weeks showed soft tissue encapsulation around the dentin particles. It can be concluded that the rabbit calvarial model used in this study is a robust model for the assessment of bone materials. Bovine dentin is a biostable material; however, it may not be suitable for repairing large 4-wall defects.
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spelling pubmed-33122612012-04-13 Evaluation of Osteoconductive and Osteogenic Potential of a Dentin-Based Bone Substitute Using a Calvarial Defect Model Hussain, Ibrahim Moharamzadeh, Keyvan Brook, Ian M. José de Oliveira Neto, Patrício Salata, Luiz A. Int J Dent Research Article The aim of this study was to assess the osteoconductive and osteogenic properties of processed bovine dentin using a robust rabbit calvarial defect model. In total, 16 New Zealand White rabbits were operated to create three circular defects in the calvaria. One defect was left unfilled, one filled with collected autogenous bone, and the third defect was filled with the dentin-based bone substitute. Following surgery and after a healing period of either 1 or 6 weeks, a CT scan was obtained. Following sacrificing, the tissues were processed for histological examination. The CT data showed the density in the area grafted with the dentin-based material was higher than the surrounding bone and the areas grafted with autologous bone after 1 week and 6 weeks of healing. The area left unfilled remained an empty defect after 1 week and 6 weeks. Histological examination of the defects filled with the dentin product after 6 weeks showed soft tissue encapsulation around the dentin particles. It can be concluded that the rabbit calvarial model used in this study is a robust model for the assessment of bone materials. Bovine dentin is a biostable material; however, it may not be suitable for repairing large 4-wall defects. Hindawi Publishing Corporation 2012 2012-03-15 /pmc/articles/PMC3312261/ /pubmed/22505899 http://dx.doi.org/10.1155/2012/396316 Text en Copyright © 2012 Ibrahim Hussain et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Hussain, Ibrahim
Moharamzadeh, Keyvan
Brook, Ian M.
José de Oliveira Neto, Patrício
Salata, Luiz A.
Evaluation of Osteoconductive and Osteogenic Potential of a Dentin-Based Bone Substitute Using a Calvarial Defect Model
title Evaluation of Osteoconductive and Osteogenic Potential of a Dentin-Based Bone Substitute Using a Calvarial Defect Model
title_full Evaluation of Osteoconductive and Osteogenic Potential of a Dentin-Based Bone Substitute Using a Calvarial Defect Model
title_fullStr Evaluation of Osteoconductive and Osteogenic Potential of a Dentin-Based Bone Substitute Using a Calvarial Defect Model
title_full_unstemmed Evaluation of Osteoconductive and Osteogenic Potential of a Dentin-Based Bone Substitute Using a Calvarial Defect Model
title_short Evaluation of Osteoconductive and Osteogenic Potential of a Dentin-Based Bone Substitute Using a Calvarial Defect Model
title_sort evaluation of osteoconductive and osteogenic potential of a dentin-based bone substitute using a calvarial defect model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3312261/
https://www.ncbi.nlm.nih.gov/pubmed/22505899
http://dx.doi.org/10.1155/2012/396316
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