Adsorption and Desorption of Bioactive Proteins on Hydroxyapatite for Protein Delivery Systems

Hydroxyapatite (HA) is a precursor of bone and has been studied as a biomaterial. We attempted HA to apply to protein delivery systems. In this study, the association and dissociation properties of two types of bioactive proteins, cytochrom c and insulin, to HA were investigated. Cytochrom c was les...

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Detalles Bibliográficos
Autores principales: Kojima, Chie, Watanabe, Kenji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3312276/
https://www.ncbi.nlm.nih.gov/pubmed/22506125
http://dx.doi.org/10.1155/2012/932461
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author Kojima, Chie
Watanabe, Kenji
author_facet Kojima, Chie
Watanabe, Kenji
author_sort Kojima, Chie
collection PubMed
description Hydroxyapatite (HA) is a precursor of bone and has been studied as a biomaterial. We attempted HA to apply to protein delivery systems. In this study, the association and dissociation properties of two types of bioactive proteins, cytochrom c and insulin, to HA were investigated. Cytochrom c was less associated with HA than insulin, which was easily released from it. However, the release of insulin from HA was slow. Insulin was released from HA at pH 7.4 more rapidly than at pH 3. The association and dissociation properties might be influenced by the size, solubility and net charge of protein. HA is a potential protein carrier with controlled release.
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spelling pubmed-33122762012-04-13 Adsorption and Desorption of Bioactive Proteins on Hydroxyapatite for Protein Delivery Systems Kojima, Chie Watanabe, Kenji J Drug Deliv Research Article Hydroxyapatite (HA) is a precursor of bone and has been studied as a biomaterial. We attempted HA to apply to protein delivery systems. In this study, the association and dissociation properties of two types of bioactive proteins, cytochrom c and insulin, to HA were investigated. Cytochrom c was less associated with HA than insulin, which was easily released from it. However, the release of insulin from HA was slow. Insulin was released from HA at pH 7.4 more rapidly than at pH 3. The association and dissociation properties might be influenced by the size, solubility and net charge of protein. HA is a potential protein carrier with controlled release. Hindawi Publishing Corporation 2012 2012-03-05 /pmc/articles/PMC3312276/ /pubmed/22506125 http://dx.doi.org/10.1155/2012/932461 Text en Copyright © 2012 C. Kojima and K. Watanabe. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kojima, Chie
Watanabe, Kenji
Adsorption and Desorption of Bioactive Proteins on Hydroxyapatite for Protein Delivery Systems
title Adsorption and Desorption of Bioactive Proteins on Hydroxyapatite for Protein Delivery Systems
title_full Adsorption and Desorption of Bioactive Proteins on Hydroxyapatite for Protein Delivery Systems
title_fullStr Adsorption and Desorption of Bioactive Proteins on Hydroxyapatite for Protein Delivery Systems
title_full_unstemmed Adsorption and Desorption of Bioactive Proteins on Hydroxyapatite for Protein Delivery Systems
title_short Adsorption and Desorption of Bioactive Proteins on Hydroxyapatite for Protein Delivery Systems
title_sort adsorption and desorption of bioactive proteins on hydroxyapatite for protein delivery systems
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3312276/
https://www.ncbi.nlm.nih.gov/pubmed/22506125
http://dx.doi.org/10.1155/2012/932461
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