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Effects of Reactive Nitrogen Scavengers on NK-Cell-Mediated Killing of K562 Cells
This study explored the effects of reactive nitrogen metabolites (RNMS) on natural-killer- (NK-) cell-mediated killing of K562 cells and the influence of RNM scavengers, such as tiopronin (TIP), glutamylcysteinylglycine (GSH), and histamine dihydrochloride (DHT), on reversing the suppressing effect...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3312280/ https://www.ncbi.nlm.nih.gov/pubmed/22505802 http://dx.doi.org/10.1155/2012/101737 |
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author | Zeng, Yili Huang, Qinmiao Zheng, Meizhu Guo, Jianxin Pan, Jingxin |
author_facet | Zeng, Yili Huang, Qinmiao Zheng, Meizhu Guo, Jianxin Pan, Jingxin |
author_sort | Zeng, Yili |
collection | PubMed |
description | This study explored the effects of reactive nitrogen metabolites (RNMS) on natural-killer- (NK-) cell-mediated killing of K562 cells and the influence of RNM scavengers, such as tiopronin (TIP), glutamylcysteinylglycine (GSH), and histamine dihydrochloride (DHT), on reversing the suppressing effect of RNM. We administered exogenous and endogenous RNM in the NK + K562 culture system and then added RNM scavengers. The concentrations of RNM, TNF-β and IFN-γ, and NK-cell cytotoxicity (NCC) and the percentage of living NK cells were then examined. We found that both exogenous and endogenous RNM caused the KIR to decrease (P < 0.01); however, RNM scavengers such as TIP and GSH rescued this phenomenon dose dependently. In conclusion, our data suggests that RNM scavengers such as TIP and GSH enhance the antineoplasmic activity of NK cells. |
format | Online Article Text |
id | pubmed-3312280 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-33122802012-04-13 Effects of Reactive Nitrogen Scavengers on NK-Cell-Mediated Killing of K562 Cells Zeng, Yili Huang, Qinmiao Zheng, Meizhu Guo, Jianxin Pan, Jingxin J Biomed Biotechnol Research Article This study explored the effects of reactive nitrogen metabolites (RNMS) on natural-killer- (NK-) cell-mediated killing of K562 cells and the influence of RNM scavengers, such as tiopronin (TIP), glutamylcysteinylglycine (GSH), and histamine dihydrochloride (DHT), on reversing the suppressing effect of RNM. We administered exogenous and endogenous RNM in the NK + K562 culture system and then added RNM scavengers. The concentrations of RNM, TNF-β and IFN-γ, and NK-cell cytotoxicity (NCC) and the percentage of living NK cells were then examined. We found that both exogenous and endogenous RNM caused the KIR to decrease (P < 0.01); however, RNM scavengers such as TIP and GSH rescued this phenomenon dose dependently. In conclusion, our data suggests that RNM scavengers such as TIP and GSH enhance the antineoplasmic activity of NK cells. Hindawi Publishing Corporation 2012 2012-02-05 /pmc/articles/PMC3312280/ /pubmed/22505802 http://dx.doi.org/10.1155/2012/101737 Text en Copyright © 2012 Yili Zeng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Zeng, Yili Huang, Qinmiao Zheng, Meizhu Guo, Jianxin Pan, Jingxin Effects of Reactive Nitrogen Scavengers on NK-Cell-Mediated Killing of K562 Cells |
title | Effects of Reactive Nitrogen Scavengers on NK-Cell-Mediated Killing of K562 Cells |
title_full | Effects of Reactive Nitrogen Scavengers on NK-Cell-Mediated Killing of K562 Cells |
title_fullStr | Effects of Reactive Nitrogen Scavengers on NK-Cell-Mediated Killing of K562 Cells |
title_full_unstemmed | Effects of Reactive Nitrogen Scavengers on NK-Cell-Mediated Killing of K562 Cells |
title_short | Effects of Reactive Nitrogen Scavengers on NK-Cell-Mediated Killing of K562 Cells |
title_sort | effects of reactive nitrogen scavengers on nk-cell-mediated killing of k562 cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3312280/ https://www.ncbi.nlm.nih.gov/pubmed/22505802 http://dx.doi.org/10.1155/2012/101737 |
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