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Rapid detection of sacbrood virus (SBV) by one-step reverse transcription loop-mediated isothermal amplification assay

BACKGROUND: Sacbrood virus (SBV) primarily infects honeybee broods, and in order to deal with the problem cost effective detection methods are required. FINDINGS: A one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid identification of SBV...

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Detalles Bibliográficos
Autores principales: Jin-Long, Yang, Rui, Yang, Ke-Fei, Shen, Xiang-Wei, Peng, Tao, Xiong, Zuo-Hua, Liu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3312826/
https://www.ncbi.nlm.nih.gov/pubmed/22340141
http://dx.doi.org/10.1186/1743-422X-9-47
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author Jin-Long, Yang
Rui, Yang
Ke-Fei, Shen
Xiang-Wei, Peng
Tao, Xiong
Zuo-Hua, Liu
author_facet Jin-Long, Yang
Rui, Yang
Ke-Fei, Shen
Xiang-Wei, Peng
Tao, Xiong
Zuo-Hua, Liu
author_sort Jin-Long, Yang
collection PubMed
description BACKGROUND: Sacbrood virus (SBV) primarily infects honeybee broods, and in order to deal with the problem cost effective detection methods are required. FINDINGS: A one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid identification of SBV. The data demonstrated that, in a simple water bath, SBV RNA could be detected as early as 20 min at 65°C, and a positive amplification reaction was visible to the naked eye due to a color change brought on by the addition of nucleic acid stain SYBR Green. CONCLUSIONS: The current study presents a method for the rapid and simple detection of SBV by RT-LAMP with high sensitivity and analytic specificity.
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spelling pubmed-33128262012-03-27 Rapid detection of sacbrood virus (SBV) by one-step reverse transcription loop-mediated isothermal amplification assay Jin-Long, Yang Rui, Yang Ke-Fei, Shen Xiang-Wei, Peng Tao, Xiong Zuo-Hua, Liu Virol J Short Report BACKGROUND: Sacbrood virus (SBV) primarily infects honeybee broods, and in order to deal with the problem cost effective detection methods are required. FINDINGS: A one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid identification of SBV. The data demonstrated that, in a simple water bath, SBV RNA could be detected as early as 20 min at 65°C, and a positive amplification reaction was visible to the naked eye due to a color change brought on by the addition of nucleic acid stain SYBR Green. CONCLUSIONS: The current study presents a method for the rapid and simple detection of SBV by RT-LAMP with high sensitivity and analytic specificity. BioMed Central 2012-02-17 /pmc/articles/PMC3312826/ /pubmed/22340141 http://dx.doi.org/10.1186/1743-422X-9-47 Text en Copyright ©2012 Jin-Long et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Report
Jin-Long, Yang
Rui, Yang
Ke-Fei, Shen
Xiang-Wei, Peng
Tao, Xiong
Zuo-Hua, Liu
Rapid detection of sacbrood virus (SBV) by one-step reverse transcription loop-mediated isothermal amplification assay
title Rapid detection of sacbrood virus (SBV) by one-step reverse transcription loop-mediated isothermal amplification assay
title_full Rapid detection of sacbrood virus (SBV) by one-step reverse transcription loop-mediated isothermal amplification assay
title_fullStr Rapid detection of sacbrood virus (SBV) by one-step reverse transcription loop-mediated isothermal amplification assay
title_full_unstemmed Rapid detection of sacbrood virus (SBV) by one-step reverse transcription loop-mediated isothermal amplification assay
title_short Rapid detection of sacbrood virus (SBV) by one-step reverse transcription loop-mediated isothermal amplification assay
title_sort rapid detection of sacbrood virus (sbv) by one-step reverse transcription loop-mediated isothermal amplification assay
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3312826/
https://www.ncbi.nlm.nih.gov/pubmed/22340141
http://dx.doi.org/10.1186/1743-422X-9-47
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