Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis)

In the present study, water buffalo MHC (Bubu)-DRB cDNA was cloned and characterized. The 1022 base long-amplified cDNA product encompassed a single open reading frame of 801 bases that coded for 266 amino acids. The Bubu-DRB sequence showed maximum homology with the BoLA-DRB3*0101 allele of cattle....

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Detalles Bibliográficos
Autores principales: Naskar, Soumen, Deb, Sitangsu M., Niranjan, Saket K., Kumar, Subodh, Sharma, Deepak, Sakaram, Durgam, Sharma, Arjava
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Genética 2012
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3313522/
https://www.ncbi.nlm.nih.gov/pubmed/22481880
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author Naskar, Soumen
Deb, Sitangsu M.
Niranjan, Saket K.
Kumar, Subodh
Sharma, Deepak
Sakaram, Durgam
Sharma, Arjava
author_facet Naskar, Soumen
Deb, Sitangsu M.
Niranjan, Saket K.
Kumar, Subodh
Sharma, Deepak
Sakaram, Durgam
Sharma, Arjava
author_sort Naskar, Soumen
collection PubMed
description In the present study, water buffalo MHC (Bubu)-DRB cDNA was cloned and characterized. The 1022 base long-amplified cDNA product encompassed a single open reading frame of 801 bases that coded for 266 amino acids. The Bubu-DRB sequence showed maximum homology with the BoLA-DRB3*0101 allele of cattle. A total of seven amino acid residues were found to be unique for the Bubu-DRB sequence. The majority of amino acid substitutions was observed in the β(1) domain. Residues associated with important functions were mostly conserved. Water buffalo DRB was phylogenetically closer to goat DRB*A.
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spelling pubmed-33135222012-04-05 Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis) Naskar, Soumen Deb, Sitangsu M. Niranjan, Saket K. Kumar, Subodh Sharma, Deepak Sakaram, Durgam Sharma, Arjava Genet Mol Biol Short Communication In the present study, water buffalo MHC (Bubu)-DRB cDNA was cloned and characterized. The 1022 base long-amplified cDNA product encompassed a single open reading frame of 801 bases that coded for 266 amino acids. The Bubu-DRB sequence showed maximum homology with the BoLA-DRB3*0101 allele of cattle. A total of seven amino acid residues were found to be unique for the Bubu-DRB sequence. The majority of amino acid substitutions was observed in the β(1) domain. Residues associated with important functions were mostly conserved. Water buffalo DRB was phylogenetically closer to goat DRB*A. Sociedade Brasileira de Genética 2012 2012-02-02 /pmc/articles/PMC3313522/ /pubmed/22481880 Text en Copyright © 2012, Sociedade Brasileira de Genética. Printed in Brazil License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Naskar, Soumen
Deb, Sitangsu M.
Niranjan, Saket K.
Kumar, Subodh
Sharma, Deepak
Sakaram, Durgam
Sharma, Arjava
Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis)
title Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis)
title_full Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis)
title_fullStr Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis)
title_full_unstemmed Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis)
title_short Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis)
title_sort molecular characterization of mhc-drb cdna in water buffalo (bubalus bubalis)
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3313522/
https://www.ncbi.nlm.nih.gov/pubmed/22481880
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