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Genetic and physical interaction of Meis2, Pax3 and Pax7 during dorsal midbrain development

BACKGROUND: During early stages of brain development, secreted molecules, components of intracellular signaling pathways and transcriptional regulators act in positive and negative feed-back or feed-forward loops at the mid-hindbrain boundary. These genetic interactions are of central importance for...

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Autores principales: Agoston, Zsuzsa, Li, Naixin, Haslinger, Anja, Wizenmann, Andrea, Schulte, Dorothea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3313853/
https://www.ncbi.nlm.nih.gov/pubmed/22390724
http://dx.doi.org/10.1186/1471-213X-12-10
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author Agoston, Zsuzsa
Li, Naixin
Haslinger, Anja
Wizenmann, Andrea
Schulte, Dorothea
author_facet Agoston, Zsuzsa
Li, Naixin
Haslinger, Anja
Wizenmann, Andrea
Schulte, Dorothea
author_sort Agoston, Zsuzsa
collection PubMed
description BACKGROUND: During early stages of brain development, secreted molecules, components of intracellular signaling pathways and transcriptional regulators act in positive and negative feed-back or feed-forward loops at the mid-hindbrain boundary. These genetic interactions are of central importance for the specification and subsequent development of the adjacent mid- and hindbrain. Much less, however, is known about the regulatory relationship and functional interaction of molecules that are expressed in the tectal anlage after tectal fate specification has taken place and tectal development has commenced. RESULTS: Here, we provide experimental evidence for reciprocal regulation and subsequent cooperation of the paired-type transcription factors Pax3, Pax7 and the TALE-homeodomain protein Meis2 in the tectal anlage. Using in ovo electroporation of the mesencephalic vesicle of chick embryos we show that (i) Pax3 and Pax7 mutually regulate each other's expression in the mesencephalic vesicle, (ii) Meis2 acts downstream of Pax3/7 and requires balanced expression levels of both proteins, and (iii) Meis2 physically interacts with Pax3 and Pax7. These results extend our previous observation that Meis2 cooperates with Otx2 in tectal development to include Pax3 and Pax7 as Meis2 interacting proteins in the tectal anlage. CONCLUSION: The results described here suggest a model in which interdependent regulatory loops involving Pax3 and Pax7 in the dorsal mesencephalic vesicle modulate Meis2 expression. Physical interaction with Meis2 may then confer tectal specificity to a wide range of otherwise broadly expressed transcriptional regulators, including Otx2, Pax3 and Pax7.
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spelling pubmed-33138532012-03-28 Genetic and physical interaction of Meis2, Pax3 and Pax7 during dorsal midbrain development Agoston, Zsuzsa Li, Naixin Haslinger, Anja Wizenmann, Andrea Schulte, Dorothea BMC Dev Biol Research Article BACKGROUND: During early stages of brain development, secreted molecules, components of intracellular signaling pathways and transcriptional regulators act in positive and negative feed-back or feed-forward loops at the mid-hindbrain boundary. These genetic interactions are of central importance for the specification and subsequent development of the adjacent mid- and hindbrain. Much less, however, is known about the regulatory relationship and functional interaction of molecules that are expressed in the tectal anlage after tectal fate specification has taken place and tectal development has commenced. RESULTS: Here, we provide experimental evidence for reciprocal regulation and subsequent cooperation of the paired-type transcription factors Pax3, Pax7 and the TALE-homeodomain protein Meis2 in the tectal anlage. Using in ovo electroporation of the mesencephalic vesicle of chick embryos we show that (i) Pax3 and Pax7 mutually regulate each other's expression in the mesencephalic vesicle, (ii) Meis2 acts downstream of Pax3/7 and requires balanced expression levels of both proteins, and (iii) Meis2 physically interacts with Pax3 and Pax7. These results extend our previous observation that Meis2 cooperates with Otx2 in tectal development to include Pax3 and Pax7 as Meis2 interacting proteins in the tectal anlage. CONCLUSION: The results described here suggest a model in which interdependent regulatory loops involving Pax3 and Pax7 in the dorsal mesencephalic vesicle modulate Meis2 expression. Physical interaction with Meis2 may then confer tectal specificity to a wide range of otherwise broadly expressed transcriptional regulators, including Otx2, Pax3 and Pax7. BioMed Central 2012-03-05 /pmc/articles/PMC3313853/ /pubmed/22390724 http://dx.doi.org/10.1186/1471-213X-12-10 Text en Copyright ©2012 Agoston et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Agoston, Zsuzsa
Li, Naixin
Haslinger, Anja
Wizenmann, Andrea
Schulte, Dorothea
Genetic and physical interaction of Meis2, Pax3 and Pax7 during dorsal midbrain development
title Genetic and physical interaction of Meis2, Pax3 and Pax7 during dorsal midbrain development
title_full Genetic and physical interaction of Meis2, Pax3 and Pax7 during dorsal midbrain development
title_fullStr Genetic and physical interaction of Meis2, Pax3 and Pax7 during dorsal midbrain development
title_full_unstemmed Genetic and physical interaction of Meis2, Pax3 and Pax7 during dorsal midbrain development
title_short Genetic and physical interaction of Meis2, Pax3 and Pax7 during dorsal midbrain development
title_sort genetic and physical interaction of meis2, pax3 and pax7 during dorsal midbrain development
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3313853/
https://www.ncbi.nlm.nih.gov/pubmed/22390724
http://dx.doi.org/10.1186/1471-213X-12-10
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