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Involvement of Iron in Biofilm Formation by Staphylococcus aureus
Staphylococcus aureus is a human pathogen that forms biofilm on catheters and medical implants. The authors' earlier study established that 1,2,3,4,6-penta-O-galloyl-β-D-glucopyranose (PGG) inhibits biofilm formation by S. aureus by preventing the initial attachment of the cells to a solid surf...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3313993/ https://www.ncbi.nlm.nih.gov/pubmed/22479621 http://dx.doi.org/10.1371/journal.pone.0034388 |
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author | Lin, Mei-Hui Shu, Jwu-Ching Huang, Hsiu-Yun Cheng, Yi-Ching |
author_facet | Lin, Mei-Hui Shu, Jwu-Ching Huang, Hsiu-Yun Cheng, Yi-Ching |
author_sort | Lin, Mei-Hui |
collection | PubMed |
description | Staphylococcus aureus is a human pathogen that forms biofilm on catheters and medical implants. The authors' earlier study established that 1,2,3,4,6-penta-O-galloyl-β-D-glucopyranose (PGG) inhibits biofilm formation by S. aureus by preventing the initial attachment of the cells to a solid surface and reducing the production of polysaccharide intercellular adhesin (PIA). Our cDNA microarray and MALDI-TOF mass spectrometric studies demonstrate that PGG treatment causes the expression of genes and proteins that are normally expressed under iron-limiting conditions. A chemical assay using ferrozine verifies that PGG is a strong iron chelator that depletes iron from the culture medium. This study finds that adding FeSO(4) to a medium that contains PGG restores the biofilm formation and the production of PIA by S. aureus SA113. The requirement of iron for biofilm formation by S. aureus SA113 can also be verified using a semi-defined medium, BM, that contains an iron chelating agent, 2, 2′-dipyridyl (2-DP). Similar to the effect of PGG, the addition of 2-DP to BM medium inhibits biofilm formation and adding FeSO(4) to BM medium that contains 2-DP restores biofilm formation. This study reveals an important mechanism of biofilm formation by S. aureus SA113. |
format | Online Article Text |
id | pubmed-3313993 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-33139932012-04-04 Involvement of Iron in Biofilm Formation by Staphylococcus aureus Lin, Mei-Hui Shu, Jwu-Ching Huang, Hsiu-Yun Cheng, Yi-Ching PLoS One Research Article Staphylococcus aureus is a human pathogen that forms biofilm on catheters and medical implants. The authors' earlier study established that 1,2,3,4,6-penta-O-galloyl-β-D-glucopyranose (PGG) inhibits biofilm formation by S. aureus by preventing the initial attachment of the cells to a solid surface and reducing the production of polysaccharide intercellular adhesin (PIA). Our cDNA microarray and MALDI-TOF mass spectrometric studies demonstrate that PGG treatment causes the expression of genes and proteins that are normally expressed under iron-limiting conditions. A chemical assay using ferrozine verifies that PGG is a strong iron chelator that depletes iron from the culture medium. This study finds that adding FeSO(4) to a medium that contains PGG restores the biofilm formation and the production of PIA by S. aureus SA113. The requirement of iron for biofilm formation by S. aureus SA113 can also be verified using a semi-defined medium, BM, that contains an iron chelating agent, 2, 2′-dipyridyl (2-DP). Similar to the effect of PGG, the addition of 2-DP to BM medium inhibits biofilm formation and adding FeSO(4) to BM medium that contains 2-DP restores biofilm formation. This study reveals an important mechanism of biofilm formation by S. aureus SA113. Public Library of Science 2012-03-27 /pmc/articles/PMC3313993/ /pubmed/22479621 http://dx.doi.org/10.1371/journal.pone.0034388 Text en Lin et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Lin, Mei-Hui Shu, Jwu-Ching Huang, Hsiu-Yun Cheng, Yi-Ching Involvement of Iron in Biofilm Formation by Staphylococcus aureus |
title | Involvement of Iron in Biofilm Formation by Staphylococcus aureus
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title_full | Involvement of Iron in Biofilm Formation by Staphylococcus aureus
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title_fullStr | Involvement of Iron in Biofilm Formation by Staphylococcus aureus
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title_full_unstemmed | Involvement of Iron in Biofilm Formation by Staphylococcus aureus
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title_short | Involvement of Iron in Biofilm Formation by Staphylococcus aureus
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title_sort | involvement of iron in biofilm formation by staphylococcus aureus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3313993/ https://www.ncbi.nlm.nih.gov/pubmed/22479621 http://dx.doi.org/10.1371/journal.pone.0034388 |
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