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Cloning and characterization of deer mouse (Peromyscus maniculatus) cytokine and chemokine cDNAs
BACKGROUND: Sin Nombre virus (SNV) establishes a persistent infection in the deer mouse, Peromyscus maniculatus. A strong antibody response occurs in response to SNV infection, but the role of the innate immune response is unclear. To address this issue, we have initiated an effort to identify and c...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2004
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC331403/ https://www.ncbi.nlm.nih.gov/pubmed/14720307 http://dx.doi.org/10.1186/1471-2172-5-1 |
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author | Schountz, Tony Green, Renata Davenport, Bennett Buniger, Amie Richens, Tiffany Root, J Jeffrey Davidson, Forbes Calisher, Charles H Beaty, Barry J |
author_facet | Schountz, Tony Green, Renata Davenport, Bennett Buniger, Amie Richens, Tiffany Root, J Jeffrey Davidson, Forbes Calisher, Charles H Beaty, Barry J |
author_sort | Schountz, Tony |
collection | PubMed |
description | BACKGROUND: Sin Nombre virus (SNV) establishes a persistent infection in the deer mouse, Peromyscus maniculatus. A strong antibody response occurs in response to SNV infection, but the role of the innate immune response is unclear. To address this issue, we have initiated an effort to identify and characterize deer mouse cytokine and chemokine genes. Such cytokines and chemokines are involved in various aspects of immunity, including the transition from innate to adaptive responses, type I and type II responses, recruitment of leukocytes to sites of infection, and production of mature cells from bone marrow progenitors. RESULTS: We established a colony of SNV antibody-negative deer mice and cloned 11 cytokine and chemokine partial cDNA sequences using directed PCR. Most of the deer mouse sequences were highly conserved with orthologous sequences from other rodent species and functional domains were identified in each putative polypeptide. CONCLUSIONS: The availability of these sequences will allow the examination of the role of these cytokines in deer mouse responses to infection with Sin Nombre virus. |
format | Text |
id | pubmed-331403 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2004 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-3314032004-02-07 Cloning and characterization of deer mouse (Peromyscus maniculatus) cytokine and chemokine cDNAs Schountz, Tony Green, Renata Davenport, Bennett Buniger, Amie Richens, Tiffany Root, J Jeffrey Davidson, Forbes Calisher, Charles H Beaty, Barry J BMC Immunol Research Article BACKGROUND: Sin Nombre virus (SNV) establishes a persistent infection in the deer mouse, Peromyscus maniculatus. A strong antibody response occurs in response to SNV infection, but the role of the innate immune response is unclear. To address this issue, we have initiated an effort to identify and characterize deer mouse cytokine and chemokine genes. Such cytokines and chemokines are involved in various aspects of immunity, including the transition from innate to adaptive responses, type I and type II responses, recruitment of leukocytes to sites of infection, and production of mature cells from bone marrow progenitors. RESULTS: We established a colony of SNV antibody-negative deer mice and cloned 11 cytokine and chemokine partial cDNA sequences using directed PCR. Most of the deer mouse sequences were highly conserved with orthologous sequences from other rodent species and functional domains were identified in each putative polypeptide. CONCLUSIONS: The availability of these sequences will allow the examination of the role of these cytokines in deer mouse responses to infection with Sin Nombre virus. BioMed Central 2004-01-13 /pmc/articles/PMC331403/ /pubmed/14720307 http://dx.doi.org/10.1186/1471-2172-5-1 Text en Copyright © 2004 Schountz et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. |
spellingShingle | Research Article Schountz, Tony Green, Renata Davenport, Bennett Buniger, Amie Richens, Tiffany Root, J Jeffrey Davidson, Forbes Calisher, Charles H Beaty, Barry J Cloning and characterization of deer mouse (Peromyscus maniculatus) cytokine and chemokine cDNAs |
title | Cloning and characterization of deer mouse (Peromyscus maniculatus) cytokine and chemokine cDNAs |
title_full | Cloning and characterization of deer mouse (Peromyscus maniculatus) cytokine and chemokine cDNAs |
title_fullStr | Cloning and characterization of deer mouse (Peromyscus maniculatus) cytokine and chemokine cDNAs |
title_full_unstemmed | Cloning and characterization of deer mouse (Peromyscus maniculatus) cytokine and chemokine cDNAs |
title_short | Cloning and characterization of deer mouse (Peromyscus maniculatus) cytokine and chemokine cDNAs |
title_sort | cloning and characterization of deer mouse (peromyscus maniculatus) cytokine and chemokine cdnas |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC331403/ https://www.ncbi.nlm.nih.gov/pubmed/14720307 http://dx.doi.org/10.1186/1471-2172-5-1 |
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