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Analysis of Expressed Sequence Tags and Characterization of a Novel Gene, Slmg7, in the Midgut of the Common Cutworm, Spodoptera litura
BACKGROUND: Out of total 3,081 assembled expressed sequence tags (ESTs) sequences representing 6,815 high-quality ESTs identified in three cDNA libraries constructed with RNA isolated from the midgut of Spodoptera litura, 1,039 ESTs showed significant hits and 1,107 ESTs did not show significant hit...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3314667/ https://www.ncbi.nlm.nih.gov/pubmed/22470457 http://dx.doi.org/10.1371/journal.pone.0033621 |
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author | He, Wen-yin Rao, Zhong-chen Zhou, Dao-hua Zheng, Si-chun Xu, Wei-hua Feng, Qi-li |
author_facet | He, Wen-yin Rao, Zhong-chen Zhou, Dao-hua Zheng, Si-chun Xu, Wei-hua Feng, Qi-li |
author_sort | He, Wen-yin |
collection | PubMed |
description | BACKGROUND: Out of total 3,081 assembled expressed sequence tags (ESTs) sequences representing 6,815 high-quality ESTs identified in three cDNA libraries constructed with RNA isolated from the midgut of Spodoptera litura, 1,039 ESTs showed significant hits and 1,107 ESTs did not show significant hits in BLAST searches. It is of interest to clarify whether or not these ESTs that did not show hits function in S. Litura. RESULTS: Twenty “no-hit” ESTs containing at least one putative open reading frame were selected for further expression analysis. The results from northern blot analysis showed that six of the selected ESTs are expressed in the larval midgut of this insect at different levels, suggesting that these ESTs represent true mRNA products, whereas the other 14 ESTs could not be detected. Homologues of the four larval midgut-predominant genes (Slmg2, Slmg7, Slmg9 and Slmg17) were detected in the genomes of other lepidopteran insects but not in Drosophila melanogaster. A novel gene, Slmg7, is expressed at a high level specifically in the midgut during each of the larval stages. Slmg7 is a single copy gene and encodes a 143-amino acids protein. The SLMG7 protein was localized to the cytoplasm of Spli-221 cells. CONCLUSIONS: Six ESTs from the no hit list are transcribed into mRNA and are mainly expressed in the midgut of S. litura. Slmg7 is a novel gene that is localized to the cytoplasm. |
format | Online Article Text |
id | pubmed-3314667 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-33146672012-04-02 Analysis of Expressed Sequence Tags and Characterization of a Novel Gene, Slmg7, in the Midgut of the Common Cutworm, Spodoptera litura He, Wen-yin Rao, Zhong-chen Zhou, Dao-hua Zheng, Si-chun Xu, Wei-hua Feng, Qi-li PLoS One Research Article BACKGROUND: Out of total 3,081 assembled expressed sequence tags (ESTs) sequences representing 6,815 high-quality ESTs identified in three cDNA libraries constructed with RNA isolated from the midgut of Spodoptera litura, 1,039 ESTs showed significant hits and 1,107 ESTs did not show significant hits in BLAST searches. It is of interest to clarify whether or not these ESTs that did not show hits function in S. Litura. RESULTS: Twenty “no-hit” ESTs containing at least one putative open reading frame were selected for further expression analysis. The results from northern blot analysis showed that six of the selected ESTs are expressed in the larval midgut of this insect at different levels, suggesting that these ESTs represent true mRNA products, whereas the other 14 ESTs could not be detected. Homologues of the four larval midgut-predominant genes (Slmg2, Slmg7, Slmg9 and Slmg17) were detected in the genomes of other lepidopteran insects but not in Drosophila melanogaster. A novel gene, Slmg7, is expressed at a high level specifically in the midgut during each of the larval stages. Slmg7 is a single copy gene and encodes a 143-amino acids protein. The SLMG7 protein was localized to the cytoplasm of Spli-221 cells. CONCLUSIONS: Six ESTs from the no hit list are transcribed into mRNA and are mainly expressed in the midgut of S. litura. Slmg7 is a novel gene that is localized to the cytoplasm. Public Library of Science 2012-03-28 /pmc/articles/PMC3314667/ /pubmed/22470457 http://dx.doi.org/10.1371/journal.pone.0033621 Text en He et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article He, Wen-yin Rao, Zhong-chen Zhou, Dao-hua Zheng, Si-chun Xu, Wei-hua Feng, Qi-li Analysis of Expressed Sequence Tags and Characterization of a Novel Gene, Slmg7, in the Midgut of the Common Cutworm, Spodoptera litura |
title | Analysis of Expressed Sequence Tags and Characterization of a Novel Gene, Slmg7, in the Midgut of the Common Cutworm, Spodoptera litura
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title_full | Analysis of Expressed Sequence Tags and Characterization of a Novel Gene, Slmg7, in the Midgut of the Common Cutworm, Spodoptera litura
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title_fullStr | Analysis of Expressed Sequence Tags and Characterization of a Novel Gene, Slmg7, in the Midgut of the Common Cutworm, Spodoptera litura
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title_full_unstemmed | Analysis of Expressed Sequence Tags and Characterization of a Novel Gene, Slmg7, in the Midgut of the Common Cutworm, Spodoptera litura
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title_short | Analysis of Expressed Sequence Tags and Characterization of a Novel Gene, Slmg7, in the Midgut of the Common Cutworm, Spodoptera litura
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title_sort | analysis of expressed sequence tags and characterization of a novel gene, slmg7, in the midgut of the common cutworm, spodoptera litura |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3314667/ https://www.ncbi.nlm.nih.gov/pubmed/22470457 http://dx.doi.org/10.1371/journal.pone.0033621 |
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