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Highly specific unnatural base pair systems as a third base pair for PCR amplification

Toward the expansion of the genetic alphabet of DNA, we present highly efficient unnatural base pair systems as an artificial third base pair for PCR. Hydrophobic unnatural base pair systems between 7-(2-thienyl)imidazo[4,5-b]pyridine (Ds) and 2-nitro-4-propynylpyrrole (Px) were fine-tuned for effic...

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Detalles Bibliográficos
Autores principales:	Yamashige, Rie, Kimoto, Michiko, Takezawa, Yusuke, Sato, Akira, Mitsui, Tsuneo, Yokoyama, Shigeyuki, Hirao, Ichiro
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2012
Materias:	Synthetic Biology and Chemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3315302/ https://www.ncbi.nlm.nih.gov/pubmed/22121213 http://dx.doi.org/10.1093/nar/gkr1068

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