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An exogenous chloroplast genome for complex sequence manipulation in algae

We demonstrate a system for cloning and modifying the chloroplast genome from the green alga, Chlamydomonas reinhardtii. Through extensive use of sequence stabilization strategies, the ex vivo genome is assembled in yeast from a collection of overlapping fragments. The assembled genome is then moved...

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Detalles Bibliográficos
Autores principales:	O'Neill, Bryan M., Mikkelson, Kari L., Gutierrez, Noel M., Cunningham, Jennifer L., Wolff, Kari L., Szyjka, Shawn J., Yohn, Christopher B., Redding, Kevin E., Mendez, Michael J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2012
Materias:	Synthetic Biology and Chemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3315318/ https://www.ncbi.nlm.nih.gov/pubmed/22116061 http://dx.doi.org/10.1093/nar/gkr1008

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