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A Quantitative, High-Throughput Reverse Genetic Screen Reveals Novel Connections between Pre–mRNA Splicing and 5′ and 3′ End Transcript Determinants

Here we present the development and implementation of a genome-wide reverse genetic screen in the budding yeast, Saccharomyces cerevisiae, that couples high-throughput strain growth, robotic RNA isolation and cDNA synthesis, and quantitative PCR to allow for a robust determination of the level of ne...

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Detalles Bibliográficos
Autores principales:	Albulescu, Laura-Oana, Sabet, Nevin, Gudipati, Mohanram, Stepankiw, Nicholas, Bergman, Zane J., Huffaker, Tim C., Pleiss, Jeffrey A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2012
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3315463/ https://www.ncbi.nlm.nih.gov/pubmed/22479188 http://dx.doi.org/10.1371/journal.pgen.1002530

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