Cargando…

Methamphetamine Increases LPS-Mediated Expression of IL-8, TNF-α and IL-1β in Human Macrophages through Common Signaling Pathways

The use of methamphetamine (MA) has increased in recent years, and is a major health concern throughout the world. The use of MA has been associated with an increased risk of acquiring HIV-1, along with an increased probability of the acquisition of various sexually transmitted infections. In order...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Xun, Silverstein, Peter S., Singh, Vijeta, Shah, Ankit, Qureshi, Nilofer, Kumar, Anil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3315580/
https://www.ncbi.nlm.nih.gov/pubmed/22479453
http://dx.doi.org/10.1371/journal.pone.0033822
_version_ 1782228261436129280
author Liu, Xun
Silverstein, Peter S.
Singh, Vijeta
Shah, Ankit
Qureshi, Nilofer
Kumar, Anil
author_facet Liu, Xun
Silverstein, Peter S.
Singh, Vijeta
Shah, Ankit
Qureshi, Nilofer
Kumar, Anil
author_sort Liu, Xun
collection PubMed
description The use of methamphetamine (MA) has increased in recent years, and is a major health concern throughout the world. The use of MA has been associated with an increased risk of acquiring HIV-1, along with an increased probability of the acquisition of various sexually transmitted infections. In order to determine the potential effects of MA exposure in the context of an infectious agent, U937 macrophages were exposed to various combinations of MA and bacterial lipopolysaccharide (LPS). Treatment with MA alone caused significant increases in the levels of TNF-α, while treatment with both MA and LPS resulted in significant increases in TNF-α, IL-1β and the chemokine IL-8. The increases in cytokine or chemokine levels seen when cells were treated with both LPS and MA were generally greater than those increases observed when cells were treated with only LPS. Treatment with chemical inhibitors demonstrated that the signal transduction pathways including NF-kB, MAPK, and PI3-Akt were involved in mediating the increased inflammatory response. As discussed in the paper, these pathways appear to be utilized by both MA and LPS, in the induction of these inflammatory mediators. Since these pathways are involved in the induction of inflammation in response to other pathogens, this suggests that MA-exacerbated inflammation may be a common feature of infectious disease in MA abusers.
format Online
Article
Text
id pubmed-3315580
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-33155802012-04-04 Methamphetamine Increases LPS-Mediated Expression of IL-8, TNF-α and IL-1β in Human Macrophages through Common Signaling Pathways Liu, Xun Silverstein, Peter S. Singh, Vijeta Shah, Ankit Qureshi, Nilofer Kumar, Anil PLoS One Research Article The use of methamphetamine (MA) has increased in recent years, and is a major health concern throughout the world. The use of MA has been associated with an increased risk of acquiring HIV-1, along with an increased probability of the acquisition of various sexually transmitted infections. In order to determine the potential effects of MA exposure in the context of an infectious agent, U937 macrophages were exposed to various combinations of MA and bacterial lipopolysaccharide (LPS). Treatment with MA alone caused significant increases in the levels of TNF-α, while treatment with both MA and LPS resulted in significant increases in TNF-α, IL-1β and the chemokine IL-8. The increases in cytokine or chemokine levels seen when cells were treated with both LPS and MA were generally greater than those increases observed when cells were treated with only LPS. Treatment with chemical inhibitors demonstrated that the signal transduction pathways including NF-kB, MAPK, and PI3-Akt were involved in mediating the increased inflammatory response. As discussed in the paper, these pathways appear to be utilized by both MA and LPS, in the induction of these inflammatory mediators. Since these pathways are involved in the induction of inflammation in response to other pathogens, this suggests that MA-exacerbated inflammation may be a common feature of infectious disease in MA abusers. Public Library of Science 2012-03-29 /pmc/articles/PMC3315580/ /pubmed/22479453 http://dx.doi.org/10.1371/journal.pone.0033822 Text en Liu et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Liu, Xun
Silverstein, Peter S.
Singh, Vijeta
Shah, Ankit
Qureshi, Nilofer
Kumar, Anil
Methamphetamine Increases LPS-Mediated Expression of IL-8, TNF-α and IL-1β in Human Macrophages through Common Signaling Pathways
title Methamphetamine Increases LPS-Mediated Expression of IL-8, TNF-α and IL-1β in Human Macrophages through Common Signaling Pathways
title_full Methamphetamine Increases LPS-Mediated Expression of IL-8, TNF-α and IL-1β in Human Macrophages through Common Signaling Pathways
title_fullStr Methamphetamine Increases LPS-Mediated Expression of IL-8, TNF-α and IL-1β in Human Macrophages through Common Signaling Pathways
title_full_unstemmed Methamphetamine Increases LPS-Mediated Expression of IL-8, TNF-α and IL-1β in Human Macrophages through Common Signaling Pathways
title_short Methamphetamine Increases LPS-Mediated Expression of IL-8, TNF-α and IL-1β in Human Macrophages through Common Signaling Pathways
title_sort methamphetamine increases lps-mediated expression of il-8, tnf-α and il-1β in human macrophages through common signaling pathways
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3315580/
https://www.ncbi.nlm.nih.gov/pubmed/22479453
http://dx.doi.org/10.1371/journal.pone.0033822
work_keys_str_mv AT liuxun methamphetamineincreaseslpsmediatedexpressionofil8tnfaandil1binhumanmacrophagesthroughcommonsignalingpathways
AT silversteinpeters methamphetamineincreaseslpsmediatedexpressionofil8tnfaandil1binhumanmacrophagesthroughcommonsignalingpathways
AT singhvijeta methamphetamineincreaseslpsmediatedexpressionofil8tnfaandil1binhumanmacrophagesthroughcommonsignalingpathways
AT shahankit methamphetamineincreaseslpsmediatedexpressionofil8tnfaandil1binhumanmacrophagesthroughcommonsignalingpathways
AT qureshinilofer methamphetamineincreaseslpsmediatedexpressionofil8tnfaandil1binhumanmacrophagesthroughcommonsignalingpathways
AT kumaranil methamphetamineincreaseslpsmediatedexpressionofil8tnfaandil1binhumanmacrophagesthroughcommonsignalingpathways