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Cdc42p and Fus2p act together late in yeast cell fusion

Cell fusion is the key event of fertilization that gives rise to the diploid zygote and is a nearly universal aspect of eukaryotic biology. In the yeast Saccharomyces cerevisiae, several mutants have been identified that are defective for cell fusion, and yet the molecular mechanism of this process...

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Autores principales: Ydenberg, Casey A., Stein, Richard A., Rose, Mark D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3315798/
https://www.ncbi.nlm.nih.gov/pubmed/22323294
http://dx.doi.org/10.1091/mbc.E11-08-0723
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author Ydenberg, Casey A.
Stein, Richard A.
Rose, Mark D.
author_facet Ydenberg, Casey A.
Stein, Richard A.
Rose, Mark D.
author_sort Ydenberg, Casey A.
collection PubMed
description Cell fusion is the key event of fertilization that gives rise to the diploid zygote and is a nearly universal aspect of eukaryotic biology. In the yeast Saccharomyces cerevisiae, several mutants have been identified that are defective for cell fusion, and yet the molecular mechanism of this process remains obscure. One obstacle has been that genetic screens have mainly focused on mating-specific factors, whereas the process likely involves housekeeping proteins as well. Here we implicate Cdc42p, an essential protein with roles in multiple aspects of morphogenesis, as a core component of the yeast cell fusion pathway. We identify a point mutant in the Rho-insert domain of CDC42, called cdc42-138, which is specifically defective in cell fusion. The cell fusion defect is not a secondary consequence of ineffective signaling or polarization. Genetic and morphological data show that Cdc42p acts at a late stage in cell fusion in concert with a key cell fusion regulator, Fus2p, which contains a Dbl-homology domain. We find that Fus2p binds specifically with activated Cdc42p, and binding is blocked by the cdc42-138 mutation. Thus, in addition to signaling and morphogenetic roles in mating, Cdc42p plays a role late in cell fusion via activation of Fus2p.
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spelling pubmed-33157982012-06-16 Cdc42p and Fus2p act together late in yeast cell fusion Ydenberg, Casey A. Stein, Richard A. Rose, Mark D. Mol Biol Cell Articles Cell fusion is the key event of fertilization that gives rise to the diploid zygote and is a nearly universal aspect of eukaryotic biology. In the yeast Saccharomyces cerevisiae, several mutants have been identified that are defective for cell fusion, and yet the molecular mechanism of this process remains obscure. One obstacle has been that genetic screens have mainly focused on mating-specific factors, whereas the process likely involves housekeeping proteins as well. Here we implicate Cdc42p, an essential protein with roles in multiple aspects of morphogenesis, as a core component of the yeast cell fusion pathway. We identify a point mutant in the Rho-insert domain of CDC42, called cdc42-138, which is specifically defective in cell fusion. The cell fusion defect is not a secondary consequence of ineffective signaling or polarization. Genetic and morphological data show that Cdc42p acts at a late stage in cell fusion in concert with a key cell fusion regulator, Fus2p, which contains a Dbl-homology domain. We find that Fus2p binds specifically with activated Cdc42p, and binding is blocked by the cdc42-138 mutation. Thus, in addition to signaling and morphogenetic roles in mating, Cdc42p plays a role late in cell fusion via activation of Fus2p. The American Society for Cell Biology 2012-04-01 /pmc/articles/PMC3315798/ /pubmed/22323294 http://dx.doi.org/10.1091/mbc.E11-08-0723 Text en © 2012 Ydenberg et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Ydenberg, Casey A.
Stein, Richard A.
Rose, Mark D.
Cdc42p and Fus2p act together late in yeast cell fusion
title Cdc42p and Fus2p act together late in yeast cell fusion
title_full Cdc42p and Fus2p act together late in yeast cell fusion
title_fullStr Cdc42p and Fus2p act together late in yeast cell fusion
title_full_unstemmed Cdc42p and Fus2p act together late in yeast cell fusion
title_short Cdc42p and Fus2p act together late in yeast cell fusion
title_sort cdc42p and fus2p act together late in yeast cell fusion
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3315798/
https://www.ncbi.nlm.nih.gov/pubmed/22323294
http://dx.doi.org/10.1091/mbc.E11-08-0723
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