Cargando…

A negative feedback loop at the nuclear periphery regulates GAL gene expression

The genome is nonrandomly organized within the nucleus, but it remains unclear how gene position affects gene expression. Silenced genes have frequently been found associated with the nuclear periphery, and the environment at the periphery is believed to be refractory to transcriptional activation....

Descripción completa

Detalles Bibliográficos
Autores principales: Green, Erin M., Jiang, Ying, Joyner, Ryan, Weis, Karsten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3315802/
https://www.ncbi.nlm.nih.gov/pubmed/22323286
http://dx.doi.org/10.1091/mbc.E11-06-0547
_version_ 1782228290648408064
author Green, Erin M.
Jiang, Ying
Joyner, Ryan
Weis, Karsten
author_facet Green, Erin M.
Jiang, Ying
Joyner, Ryan
Weis, Karsten
author_sort Green, Erin M.
collection PubMed
description The genome is nonrandomly organized within the nucleus, but it remains unclear how gene position affects gene expression. Silenced genes have frequently been found associated with the nuclear periphery, and the environment at the periphery is believed to be refractory to transcriptional activation. However, in budding yeast, several highly regulated classes of genes, including the GAL7-10-1 gene cluster, are known to translocate to the nuclear periphery concurrent with their activation. To investigate the role of gene positioning on GAL gene expression, we monitored the effects of mutations that disrupt the interaction between the GAL locus and the periphery or synthetically tethered the locus to the periphery. Localization to the nuclear periphery was found to dampen initial GAL gene induction and was required for rapid repression after gene inactivation, revealing a function for the nuclear periphery in repressing endogenous GAL gene expression. Our results do not support a gene-gating model in which GAL gene interaction with the nuclear pore ensures rapid gene expression, but instead they suggest that a repressive environment at the nuclear periphery establishes a negative feedback loop that enables the GAL locus to respond rapidly to changes in environmental conditions.
format Online
Article
Text
id pubmed-3315802
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher The American Society for Cell Biology
record_format MEDLINE/PubMed
spelling pubmed-33158022012-06-16 A negative feedback loop at the nuclear periphery regulates GAL gene expression Green, Erin M. Jiang, Ying Joyner, Ryan Weis, Karsten Mol Biol Cell Articles The genome is nonrandomly organized within the nucleus, but it remains unclear how gene position affects gene expression. Silenced genes have frequently been found associated with the nuclear periphery, and the environment at the periphery is believed to be refractory to transcriptional activation. However, in budding yeast, several highly regulated classes of genes, including the GAL7-10-1 gene cluster, are known to translocate to the nuclear periphery concurrent with their activation. To investigate the role of gene positioning on GAL gene expression, we monitored the effects of mutations that disrupt the interaction between the GAL locus and the periphery or synthetically tethered the locus to the periphery. Localization to the nuclear periphery was found to dampen initial GAL gene induction and was required for rapid repression after gene inactivation, revealing a function for the nuclear periphery in repressing endogenous GAL gene expression. Our results do not support a gene-gating model in which GAL gene interaction with the nuclear pore ensures rapid gene expression, but instead they suggest that a repressive environment at the nuclear periphery establishes a negative feedback loop that enables the GAL locus to respond rapidly to changes in environmental conditions. The American Society for Cell Biology 2012-04-01 /pmc/articles/PMC3315802/ /pubmed/22323286 http://dx.doi.org/10.1091/mbc.E11-06-0547 Text en © 2012 Green et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Green, Erin M.
Jiang, Ying
Joyner, Ryan
Weis, Karsten
A negative feedback loop at the nuclear periphery regulates GAL gene expression
title A negative feedback loop at the nuclear periphery regulates GAL gene expression
title_full A negative feedback loop at the nuclear periphery regulates GAL gene expression
title_fullStr A negative feedback loop at the nuclear periphery regulates GAL gene expression
title_full_unstemmed A negative feedback loop at the nuclear periphery regulates GAL gene expression
title_short A negative feedback loop at the nuclear periphery regulates GAL gene expression
title_sort negative feedback loop at the nuclear periphery regulates gal gene expression
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3315802/
https://www.ncbi.nlm.nih.gov/pubmed/22323286
http://dx.doi.org/10.1091/mbc.E11-06-0547
work_keys_str_mv AT greenerinm anegativefeedbackloopatthenuclearperipheryregulatesgalgeneexpression
AT jiangying anegativefeedbackloopatthenuclearperipheryregulatesgalgeneexpression
AT joynerryan anegativefeedbackloopatthenuclearperipheryregulatesgalgeneexpression
AT weiskarsten anegativefeedbackloopatthenuclearperipheryregulatesgalgeneexpression
AT greenerinm negativefeedbackloopatthenuclearperipheryregulatesgalgeneexpression
AT jiangying negativefeedbackloopatthenuclearperipheryregulatesgalgeneexpression
AT joynerryan negativefeedbackloopatthenuclearperipheryregulatesgalgeneexpression
AT weiskarsten negativefeedbackloopatthenuclearperipheryregulatesgalgeneexpression