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Chemical Clearing and Dehydration of GFP Expressing Mouse Brains

Generally, chemical tissue clearing is performed by a solution consisting of two parts benzyl benzoate and one part benzyl alcohol. However, prolonged exposure to this mixture markedly reduces the fluorescence of GFP expressing specimens, so that one has to compromise between clearing quality and fl...

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Detalles Bibliográficos
Autores principales: Becker, Klaus, Jährling, Nina, Saghafi, Saiedeh, Weiler, Reto, Dodt, Hans-Ulrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3316521/
https://www.ncbi.nlm.nih.gov/pubmed/22479475
http://dx.doi.org/10.1371/journal.pone.0033916