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Expanding the Chemical Cross-Linking Toolbox by the Use of Multiple Proteases and Enrichment by Size Exclusion Chromatography
Chemical cross-linking in combination with mass spectrometric analysis offers the potential to obtain low-resolution structural information from proteins and protein complexes. Identification of peptides connected by a cross-link provides direct evidence for the physical interaction of amino acid si...
Autores principales: | Leitner, Alexander, Reischl, Roland, Walzthoeni, Thomas, Herzog, Franz, Bohn, Stefan, Förster, Friedrich, Aebersold, Ruedi |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Biochemistry and Molecular Biology
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3316732/ https://www.ncbi.nlm.nih.gov/pubmed/22286754 http://dx.doi.org/10.1074/mcp.M111.014126 |
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