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Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%
Cyan variants of green fluorescent protein are widely used as donors in Förster resonance energy transfer experiments. The popular, but modestly bright, Enhanced Cyan Fluorescent Protein (ECFP) was sequentially improved into the brighter variants Super Cyan Fluorescent Protein 3A (SCFP3A) and mTurqu...
Autores principales: | Goedhart, Joachim, von Stetten, David, Noirclerc-Savoye, Marjolaine, Lelimousin, Mickaël, Joosen, Linda, Hink, Mark A., van Weeren, Laura, Gadella, Theodorus W.J., Royant, Antoine |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Pub. Group
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3316892/ https://www.ncbi.nlm.nih.gov/pubmed/22434194 http://dx.doi.org/10.1038/ncomms1738 |
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