Cargando…

Immunolocalization of NLRP3 Inflammasome in Normal Murine Airway Epithelium and Changes following Induction of Ovalbumin-Induced Airway Inflammation

Little is known about innate immunity and components of inflammasomes in airway epithelium. This study evaluated immunohistological evidence for NLRP3 inflammasomes in normal and inflamed murine (Balb/c) airway epithelium in a model of ovalbumin (OVA) induced allergic airway inflammation. The airway...

Descripción completa

Detalles Bibliográficos
Autores principales: Tran, Hai B., Lewis, Martin D., Tan, Lor Wai, Lester, Susan E., Baker, Leonie M., Ng, Jia, Hamilton-Bruce, Monica A., Hill, Catherine L., Koblar, Simon A., Rischmueller, Maureen, Ruffin, Richard E., Wormald, Peter J., Zalewski, Peter D., Lang, Carol J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317233/
https://www.ncbi.nlm.nih.gov/pubmed/22523501
http://dx.doi.org/10.1155/2012/819176
_version_ 1782228534609051648
author Tran, Hai B.
Lewis, Martin D.
Tan, Lor Wai
Lester, Susan E.
Baker, Leonie M.
Ng, Jia
Hamilton-Bruce, Monica A.
Hill, Catherine L.
Koblar, Simon A.
Rischmueller, Maureen
Ruffin, Richard E.
Wormald, Peter J.
Zalewski, Peter D.
Lang, Carol J.
author_facet Tran, Hai B.
Lewis, Martin D.
Tan, Lor Wai
Lester, Susan E.
Baker, Leonie M.
Ng, Jia
Hamilton-Bruce, Monica A.
Hill, Catherine L.
Koblar, Simon A.
Rischmueller, Maureen
Ruffin, Richard E.
Wormald, Peter J.
Zalewski, Peter D.
Lang, Carol J.
author_sort Tran, Hai B.
collection PubMed
description Little is known about innate immunity and components of inflammasomes in airway epithelium. This study evaluated immunohistological evidence for NLRP3 inflammasomes in normal and inflamed murine (Balb/c) airway epithelium in a model of ovalbumin (OVA) induced allergic airway inflammation. The airway epithelium of control mice exhibited strong cytoplasmic staining for total caspase-1, ASC, and NLRP3, whereas the OVA mice exhibited strong staining for active caspase-1, with redistribution of caspase-1, IL-1β and IL-18, indicating possible activation of the NLRP3 inflammasome. Active caspase-1, NLRP3, and other inflammasome components were also detected in tissue eosinophils from OVA mice, and may potentially contribute to IL-1β and IL-18 production. In whole lung, inRNA expression of NAIP and procaspase-1 was increased in OVA mice, whereas NLRP3, IL-1β and IL-18 decreased. Some OVA-treated mice also had significantly elevated and tightly correlated serum levels of IL-1β and TNFα. In cultured normal human bronchial epithelial cells, LPS priming resulted in a significant increase in NLRP3 and II-lp protein expression. This study is the first to demonstrate NLRP3 inflammasome components in normal airway epithelium and changes with inflammation. We propose activation and/or luminal release of the inflammasome is a feature of allergic airway inflammation which may contribute to disease pathogenesis.
format Online
Article
Text
id pubmed-3317233
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-33172332012-04-20 Immunolocalization of NLRP3 Inflammasome in Normal Murine Airway Epithelium and Changes following Induction of Ovalbumin-Induced Airway Inflammation Tran, Hai B. Lewis, Martin D. Tan, Lor Wai Lester, Susan E. Baker, Leonie M. Ng, Jia Hamilton-Bruce, Monica A. Hill, Catherine L. Koblar, Simon A. Rischmueller, Maureen Ruffin, Richard E. Wormald, Peter J. Zalewski, Peter D. Lang, Carol J. J Allergy (Cairo) Research Article Little is known about innate immunity and components of inflammasomes in airway epithelium. This study evaluated immunohistological evidence for NLRP3 inflammasomes in normal and inflamed murine (Balb/c) airway epithelium in a model of ovalbumin (OVA) induced allergic airway inflammation. The airway epithelium of control mice exhibited strong cytoplasmic staining for total caspase-1, ASC, and NLRP3, whereas the OVA mice exhibited strong staining for active caspase-1, with redistribution of caspase-1, IL-1β and IL-18, indicating possible activation of the NLRP3 inflammasome. Active caspase-1, NLRP3, and other inflammasome components were also detected in tissue eosinophils from OVA mice, and may potentially contribute to IL-1β and IL-18 production. In whole lung, inRNA expression of NAIP and procaspase-1 was increased in OVA mice, whereas NLRP3, IL-1β and IL-18 decreased. Some OVA-treated mice also had significantly elevated and tightly correlated serum levels of IL-1β and TNFα. In cultured normal human bronchial epithelial cells, LPS priming resulted in a significant increase in NLRP3 and II-lp protein expression. This study is the first to demonstrate NLRP3 inflammasome components in normal airway epithelium and changes with inflammation. We propose activation and/or luminal release of the inflammasome is a feature of allergic airway inflammation which may contribute to disease pathogenesis. Hindawi Publishing Corporation 2012 2012-03-18 /pmc/articles/PMC3317233/ /pubmed/22523501 http://dx.doi.org/10.1155/2012/819176 Text en Copyright © 2012 Hai B. Tran et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Tran, Hai B.
Lewis, Martin D.
Tan, Lor Wai
Lester, Susan E.
Baker, Leonie M.
Ng, Jia
Hamilton-Bruce, Monica A.
Hill, Catherine L.
Koblar, Simon A.
Rischmueller, Maureen
Ruffin, Richard E.
Wormald, Peter J.
Zalewski, Peter D.
Lang, Carol J.
Immunolocalization of NLRP3 Inflammasome in Normal Murine Airway Epithelium and Changes following Induction of Ovalbumin-Induced Airway Inflammation
title Immunolocalization of NLRP3 Inflammasome in Normal Murine Airway Epithelium and Changes following Induction of Ovalbumin-Induced Airway Inflammation
title_full Immunolocalization of NLRP3 Inflammasome in Normal Murine Airway Epithelium and Changes following Induction of Ovalbumin-Induced Airway Inflammation
title_fullStr Immunolocalization of NLRP3 Inflammasome in Normal Murine Airway Epithelium and Changes following Induction of Ovalbumin-Induced Airway Inflammation
title_full_unstemmed Immunolocalization of NLRP3 Inflammasome in Normal Murine Airway Epithelium and Changes following Induction of Ovalbumin-Induced Airway Inflammation
title_short Immunolocalization of NLRP3 Inflammasome in Normal Murine Airway Epithelium and Changes following Induction of Ovalbumin-Induced Airway Inflammation
title_sort immunolocalization of nlrp3 inflammasome in normal murine airway epithelium and changes following induction of ovalbumin-induced airway inflammation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317233/
https://www.ncbi.nlm.nih.gov/pubmed/22523501
http://dx.doi.org/10.1155/2012/819176
work_keys_str_mv AT tranhaib immunolocalizationofnlrp3inflammasomeinnormalmurineairwayepitheliumandchangesfollowinginductionofovalbumininducedairwayinflammation
AT lewismartind immunolocalizationofnlrp3inflammasomeinnormalmurineairwayepitheliumandchangesfollowinginductionofovalbumininducedairwayinflammation
AT tanlorwai immunolocalizationofnlrp3inflammasomeinnormalmurineairwayepitheliumandchangesfollowinginductionofovalbumininducedairwayinflammation
AT lestersusane immunolocalizationofnlrp3inflammasomeinnormalmurineairwayepitheliumandchangesfollowinginductionofovalbumininducedairwayinflammation
AT bakerleoniem immunolocalizationofnlrp3inflammasomeinnormalmurineairwayepitheliumandchangesfollowinginductionofovalbumininducedairwayinflammation
AT ngjia immunolocalizationofnlrp3inflammasomeinnormalmurineairwayepitheliumandchangesfollowinginductionofovalbumininducedairwayinflammation
AT hamiltonbrucemonicaa immunolocalizationofnlrp3inflammasomeinnormalmurineairwayepitheliumandchangesfollowinginductionofovalbumininducedairwayinflammation
AT hillcatherinel immunolocalizationofnlrp3inflammasomeinnormalmurineairwayepitheliumandchangesfollowinginductionofovalbumininducedairwayinflammation
AT koblarsimona immunolocalizationofnlrp3inflammasomeinnormalmurineairwayepitheliumandchangesfollowinginductionofovalbumininducedairwayinflammation
AT rischmuellermaureen immunolocalizationofnlrp3inflammasomeinnormalmurineairwayepitheliumandchangesfollowinginductionofovalbumininducedairwayinflammation
AT ruffinricharde immunolocalizationofnlrp3inflammasomeinnormalmurineairwayepitheliumandchangesfollowinginductionofovalbumininducedairwayinflammation
AT wormaldpeterj immunolocalizationofnlrp3inflammasomeinnormalmurineairwayepitheliumandchangesfollowinginductionofovalbumininducedairwayinflammation
AT zalewskipeterd immunolocalizationofnlrp3inflammasomeinnormalmurineairwayepitheliumandchangesfollowinginductionofovalbumininducedairwayinflammation
AT langcarolj immunolocalizationofnlrp3inflammasomeinnormalmurineairwayepitheliumandchangesfollowinginductionofovalbumininducedairwayinflammation