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STING Mediates Neuronal Innate Immune Response Following Japanese Encephalitis Virus Infection

Flavivirus-mediated inflammation causes neuronal death, but whether the infected neurons can evoke an innate immune response to elicit their own protection, is unknown. In an earlier study we have shown that neuronal RIG-I, play a significant role in inducing production and release of molecules that...

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Autores principales: Nazmi, Arshed, Mukhopadhyay, Rupanjan, Dutta, Kallol, Basu, Anirban
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317237/
https://www.ncbi.nlm.nih.gov/pubmed/22470840
http://dx.doi.org/10.1038/srep00347
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author Nazmi, Arshed
Mukhopadhyay, Rupanjan
Dutta, Kallol
Basu, Anirban
author_facet Nazmi, Arshed
Mukhopadhyay, Rupanjan
Dutta, Kallol
Basu, Anirban
author_sort Nazmi, Arshed
collection PubMed
description Flavivirus-mediated inflammation causes neuronal death, but whether the infected neurons can evoke an innate immune response to elicit their own protection, is unknown. In an earlier study we have shown that neuronal RIG-I, play a significant role in inducing production and release of molecules that are related to inflammation. In this study, using a neuronal cell line, we show that RIG-I acts with STING in a concerted manner following its interaction with Japanese encephalitis viral RNA to induce a type 1 interferon response. Knock-down of STING showed that the expressions of various inflammatory signaling molecules were down-regulated along with increased intracellular viral load. Alternatively, over-expressing STING decreased intracellular viral load. Our results indicate that at the sub-cellular level, interaction between the pattern recognition receptor RIG-I and the adapter molecule STING, is a major contributor to elicit immunological responses involving the type 1 interferons in neurons following JEV infections.
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spelling pubmed-33172372012-04-02 STING Mediates Neuronal Innate Immune Response Following Japanese Encephalitis Virus Infection Nazmi, Arshed Mukhopadhyay, Rupanjan Dutta, Kallol Basu, Anirban Sci Rep Article Flavivirus-mediated inflammation causes neuronal death, but whether the infected neurons can evoke an innate immune response to elicit their own protection, is unknown. In an earlier study we have shown that neuronal RIG-I, play a significant role in inducing production and release of molecules that are related to inflammation. In this study, using a neuronal cell line, we show that RIG-I acts with STING in a concerted manner following its interaction with Japanese encephalitis viral RNA to induce a type 1 interferon response. Knock-down of STING showed that the expressions of various inflammatory signaling molecules were down-regulated along with increased intracellular viral load. Alternatively, over-expressing STING decreased intracellular viral load. Our results indicate that at the sub-cellular level, interaction between the pattern recognition receptor RIG-I and the adapter molecule STING, is a major contributor to elicit immunological responses involving the type 1 interferons in neurons following JEV infections. Nature Publishing Group 2012-04-02 /pmc/articles/PMC3317237/ /pubmed/22470840 http://dx.doi.org/10.1038/srep00347 Text en Copyright © 2012, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareALike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
spellingShingle Article
Nazmi, Arshed
Mukhopadhyay, Rupanjan
Dutta, Kallol
Basu, Anirban
STING Mediates Neuronal Innate Immune Response Following Japanese Encephalitis Virus Infection
title STING Mediates Neuronal Innate Immune Response Following Japanese Encephalitis Virus Infection
title_full STING Mediates Neuronal Innate Immune Response Following Japanese Encephalitis Virus Infection
title_fullStr STING Mediates Neuronal Innate Immune Response Following Japanese Encephalitis Virus Infection
title_full_unstemmed STING Mediates Neuronal Innate Immune Response Following Japanese Encephalitis Virus Infection
title_short STING Mediates Neuronal Innate Immune Response Following Japanese Encephalitis Virus Infection
title_sort sting mediates neuronal innate immune response following japanese encephalitis virus infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317237/
https://www.ncbi.nlm.nih.gov/pubmed/22470840
http://dx.doi.org/10.1038/srep00347
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