Characterization of the antiproliferative activity of Xylopia aethiopica

BACKGROUND: Xylopia aethiopica, a plant found throughout West Africa, has both nutritional and medicinal uses. The present study aims to characterize the effects of extracts of this plant on cancer cells. RESULTS: We report that X. aethiopica extract prepared with 70% ethanol has antiproliferative a...

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Autores principales: Choumessi, Aphrodite T, Danel, Mathieu, Chassaing, Stefan, Truchet, Isabelle, Penlap, Véronique B, Pieme, Anatole Constant, Asonganyi, Tazoacha, Ducommun, Bernard, Valette, Annie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317441/
https://www.ncbi.nlm.nih.gov/pubmed/22409878
http://dx.doi.org/10.1186/1747-1028-7-8
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author Choumessi, Aphrodite T
Danel, Mathieu
Chassaing, Stefan
Truchet, Isabelle
Penlap, Véronique B
Pieme, Anatole Constant
Asonganyi, Tazoacha
Ducommun, Bernard
Valette, Annie
author_facet Choumessi, Aphrodite T
Danel, Mathieu
Chassaing, Stefan
Truchet, Isabelle
Penlap, Véronique B
Pieme, Anatole Constant
Asonganyi, Tazoacha
Ducommun, Bernard
Valette, Annie
author_sort Choumessi, Aphrodite T
collection PubMed
description BACKGROUND: Xylopia aethiopica, a plant found throughout West Africa, has both nutritional and medicinal uses. The present study aims to characterize the effects of extracts of this plant on cancer cells. RESULTS: We report that X. aethiopica extract prepared with 70% ethanol has antiproliferative activity against a panel of cancer cell lines. The IC50 was estimated at 12 μg/ml against HCT116 colon cancer cells, 7.5 μg/ml and > 25 μg/ml against U937 and KG1a leukemia cells, respectively. Upon fractionation of the extract by HPLC, the active fraction induced DNA damage, cell cycle arrest in G1 phase and apoptotic cell death. By using NMR and mass spectrometry, we determined the structure of the active natural product in the HPLC fraction as ent-15-oxokaur-16-en-19-oic acid. CONCLUSION: The main cytotoxic and DNA-damaging compound in ethanolic extracts of Xylopia aethiopica is ent-15-oxokaur-16-en-19-oic acid.
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spelling pubmed-33174412012-04-03 Characterization of the antiproliferative activity of Xylopia aethiopica Choumessi, Aphrodite T Danel, Mathieu Chassaing, Stefan Truchet, Isabelle Penlap, Véronique B Pieme, Anatole Constant Asonganyi, Tazoacha Ducommun, Bernard Valette, Annie Cell Div Research BACKGROUND: Xylopia aethiopica, a plant found throughout West Africa, has both nutritional and medicinal uses. The present study aims to characterize the effects of extracts of this plant on cancer cells. RESULTS: We report that X. aethiopica extract prepared with 70% ethanol has antiproliferative activity against a panel of cancer cell lines. The IC50 was estimated at 12 μg/ml against HCT116 colon cancer cells, 7.5 μg/ml and > 25 μg/ml against U937 and KG1a leukemia cells, respectively. Upon fractionation of the extract by HPLC, the active fraction induced DNA damage, cell cycle arrest in G1 phase and apoptotic cell death. By using NMR and mass spectrometry, we determined the structure of the active natural product in the HPLC fraction as ent-15-oxokaur-16-en-19-oic acid. CONCLUSION: The main cytotoxic and DNA-damaging compound in ethanolic extracts of Xylopia aethiopica is ent-15-oxokaur-16-en-19-oic acid. BioMed Central 2012-03-12 /pmc/articles/PMC3317441/ /pubmed/22409878 http://dx.doi.org/10.1186/1747-1028-7-8 Text en Copyright ©2012 Choumessi et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Choumessi, Aphrodite T
Danel, Mathieu
Chassaing, Stefan
Truchet, Isabelle
Penlap, Véronique B
Pieme, Anatole Constant
Asonganyi, Tazoacha
Ducommun, Bernard
Valette, Annie
Characterization of the antiproliferative activity of Xylopia aethiopica
title Characterization of the antiproliferative activity of Xylopia aethiopica
title_full Characterization of the antiproliferative activity of Xylopia aethiopica
title_fullStr Characterization of the antiproliferative activity of Xylopia aethiopica
title_full_unstemmed Characterization of the antiproliferative activity of Xylopia aethiopica
title_short Characterization of the antiproliferative activity of Xylopia aethiopica
title_sort characterization of the antiproliferative activity of xylopia aethiopica
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317441/
https://www.ncbi.nlm.nih.gov/pubmed/22409878
http://dx.doi.org/10.1186/1747-1028-7-8
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